Using whole mount in situ hybridization to examine thyroid hormone deiodinase expression in embryonic and larval zebrafish: A tool for examining OH-BDE toxicity to early life stages

► Whole mount in situ hybridization (WISH) identifies regional and developmental deiodinase mRNA expression in zebrafish ► Deiodinase 1 and 2 were localized in periventricular zone of the brain in zebrafish embryos ► Deiodinase 3 was localized in the pronephric duct of zebrafish embryos ► 6-OH-BDE-4...

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Veröffentlicht in:	Aquatic toxicology 2013-05, Vol.132-133, p.190-199
Hauptverfasser:	Dong, Wu, Macaulay, Laura J., Kwok, Kevin W.H., Hinton, David E., Stapleton, Heather M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences brain color computer software Danio rerio Dose-Response Relationship, Drug drug effects early development Ecotoxicology, biological effects of pollution Embryo, Nonmammalian Embryo, Nonmammalian - embryology Embryo, Nonmammalian - metabolism embryology Endocrine Disruptors enzymes fish Fish Proteins Fish Proteins - metabolism Flame Retardants Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation Gene Expression Regulation - drug effects General aspects genes growth & development in situ hybridization In Situ Hybridization - methods Iodide Peroxidase Iodide Peroxidase - metabolism Larva Larva - growth & development Larva - metabolism larvae mammals messenger RNA metabolism metabolites methods neurodevelopment pollutants Polybrominated Biphenyls Polybrominated Biphenyls - toxicity polybrominated diphenyl ethers Protein Isoforms Protein Isoforms - metabolism RNA, Messenger RNA, Messenger - metabolism thyroid hormones toxicity Water Pollutants, Chemical Water Pollutants, Chemical - toxicity Whole mount in situ hybridization Zebrafish Zebrafish - growth & development Zebrafish - metabolism
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