Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: Mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15

► Porcine CD3 ectodomain molecules were expressed in Escherichia coli and refolded in vitro. ► The refolded porcine CD3 ectodomain molecules were purified with a strong anion exchange resin. ► Porcine CD3εγ ectodomain single-chain fusion protein can bind to an anti-porcine CD3 mAb 898H2-6-15. The po...

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Veröffentlicht in:	Cellular immunology 2012-03, Vol.276 (1-2), p.162-167
Hauptverfasser:	Peraino, Jaclyn Stromp, Hermanrud, Christina E., Springett, Lauren, Zhang, Huiping, Li, Guoying, Srinivasan, Srimathi, Gusha, Ashley, Sachs, David H., Huang, Christene A., Wang, Zhirui
Format:	Artikel
Sprache:	eng
Schlagworte:	898H2-6-15 monoclonal antibody Animals Antibodies, Monoclonal - immunology Binding, Competitive CD3 Complex - genetics CD3 Complex - immunology Epitope Mapping Gene Expression Immunotoxin Porcine CD3 Recombinant Proteins - genetics Recombinant Proteins - immunology Solubility Swine - immunology T cell
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