Targeting Id1 reduces proliferation and invasion in aggressive human salivary gland cancer cells
Salivary gland cancer (SGC) is one of the common malignancies of the head and neck area. It develops in the minor and major salivary glands and sometimes metastasizes to other organs, particularly to the lungs. Inhibitors of differentiation (Id) proteins are negative regulators of basic helix-loop-h...
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| Veröffentlicht in: | BMC cancer 2013-03, Vol.13 (1), p.141-141, Article 141 |
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| creator | Sumida, Tomoki Murase, Ryuichi Onishi-Ishikawa, Akiko McAllister, Sean D Hamakawa, Hiroyuki Desprez, Pierre-Yves |
| description | Salivary gland cancer (SGC) is one of the common malignancies of the head and neck area. It develops in the minor and major salivary glands and sometimes metastasizes to other organs, particularly to the lungs. Inhibitors of differentiation (Id) proteins are negative regulators of basic helix-loop-helix transcription factors that control malignant cell behavior and tumor aggressiveness in many tissues. In this study, our goal was to determine the potential role of Id proteins, particularly Id1, during human SGC cell progression.
We first determined the expression levels of Id1 and Id2 in four SGC cell lines: two adenocarcinoma of the salivary gland (HSG and HSY) and two adenoid cystic carcinoma (ACC2 and ACCM) cell lines. We then used constructs that expressed antisense cDNAs to Id1 or Id2 to knockdown the expression of these proteins in cell lines where they were highly expressed, and determined the effects of the knockdown on cell proliferation, migration and invasion.
Id1 mRNA and protein were detectable in all cell lines, and expression of Id2 was variable, from absent to high. The ACC2 and ACCM cell lines expressed both Id1 and Id2, but Id1 was expressed at a higher level in the more aggressive ACCM cell line in comparison to ACC2 cells as confirmed by Id1 promoter-reporter assays. We therefore focused on the ACCM cells for the remainder of the study. We found that proliferation and invasiveness of ACCM cells were strongly reduced after Id1 knockdown whereas Id2 suppression had only a slight effect. Results of scratch and colony formation assays also confirmed that ACCM cell aggressiveness was significantly reduced upon Id1 knockdown. Finally, this knockdown resulted in reduced c-myc and enhanced cyclin-dependent kinase inhibitor p21 expression.
These results demonstrate that Id1 plays an important role in the control of human SGC cell aggressiveness and suggest a potential role as a marker of diagnosis, prognosis and progression of SGCs. Id1 suppression could represent a novel and effective approach for the treatment of salivary gland cancer. |
| doi_str_mv | 10.1186/1471-2407-13-141 |
| format | Article |
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We first determined the expression levels of Id1 and Id2 in four SGC cell lines: two adenocarcinoma of the salivary gland (HSG and HSY) and two adenoid cystic carcinoma (ACC2 and ACCM) cell lines. We then used constructs that expressed antisense cDNAs to Id1 or Id2 to knockdown the expression of these proteins in cell lines where they were highly expressed, and determined the effects of the knockdown on cell proliferation, migration and invasion.
Id1 mRNA and protein were detectable in all cell lines, and expression of Id2 was variable, from absent to high. The ACC2 and ACCM cell lines expressed both Id1 and Id2, but Id1 was expressed at a higher level in the more aggressive ACCM cell line in comparison to ACC2 cells as confirmed by Id1 promoter-reporter assays. We therefore focused on the ACCM cells for the remainder of the study. We found that proliferation and invasiveness of ACCM cells were strongly reduced after Id1 knockdown whereas Id2 suppression had only a slight effect. Results of scratch and colony formation assays also confirmed that ACCM cell aggressiveness was significantly reduced upon Id1 knockdown. Finally, this knockdown resulted in reduced c-myc and enhanced cyclin-dependent kinase inhibitor p21 expression.
These results demonstrate that Id1 plays an important role in the control of human SGC cell aggressiveness and suggest a potential role as a marker of diagnosis, prognosis and progression of SGCs. Id1 suppression could represent a novel and effective approach for the treatment of salivary gland cancer.</description><identifier>ISSN: 1471-2407</identifier><identifier>EISSN: 1471-2407</identifier><identifier>DOI: 10.1186/1471-2407-13-141</identifier><identifier>PMID: 23517130</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Analysis ; Cancer ; Carcinoma, Adenoid Cystic - genetics ; Carcinoma, Adenoid Cystic - metabolism ; Cell adhesion & migration ; Cell culture ; Cell cycle ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Cyclin-Dependent Kinase Inhibitor p21 - genetics ; DNA binding proteins ; Down-Regulation ; Gene expression ; Gene Knockdown Techniques ; Genotype & phenotype ; Humans ; Inhibitor of Differentiation Protein 1 - genetics ; Inhibitor of Differentiation Protein 1 - metabolism ; Inhibitor of Differentiation Protein 2 - genetics ; Inhibitor of Differentiation Protein 2 - metabolism ; Insulin ; Membranes ; Physiological aspects ; Proteins ; Proto-Oncogene Proteins c-myc - genetics ; Regulation ; Salivary Gland Neoplasms - genetics ; Salivary Gland Neoplasms - metabolism ; Salivary gland tumors ; Transcription factors ; Tumor Stem Cell Assay ; Tumors ; Up-Regulation</subject><ispartof>BMC cancer, 2013-03, Vol.13 (1), p.141-141, Article 141</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Sumida et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Sumida et al.; licensee BioMed Central Ltd. 2013 Sumida et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c622t-90052975e9ec4a8391bb1e061a2d1d5288d631446ef07a3d943c939765025c5b3</citedby><cites>FETCH-LOGICAL-c622t-90052975e9ec4a8391bb1e061a2d1d5288d631446ef07a3d943c939765025c5b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639030/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639030/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23517130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sumida, Tomoki</creatorcontrib><creatorcontrib>Murase, Ryuichi</creatorcontrib><creatorcontrib>Onishi-Ishikawa, Akiko</creatorcontrib><creatorcontrib>McAllister, Sean D</creatorcontrib><creatorcontrib>Hamakawa, Hiroyuki</creatorcontrib><creatorcontrib>Desprez, Pierre-Yves</creatorcontrib><title>Targeting Id1 reduces proliferation and invasion in aggressive human salivary gland cancer cells</title><title>BMC cancer</title><addtitle>BMC Cancer</addtitle><description>Salivary gland cancer (SGC) is one of the common malignancies of the head and neck area. It develops in the minor and major salivary glands and sometimes metastasizes to other organs, particularly to the lungs. Inhibitors of differentiation (Id) proteins are negative regulators of basic helix-loop-helix transcription factors that control malignant cell behavior and tumor aggressiveness in many tissues. In this study, our goal was to determine the potential role of Id proteins, particularly Id1, during human SGC cell progression.
We first determined the expression levels of Id1 and Id2 in four SGC cell lines: two adenocarcinoma of the salivary gland (HSG and HSY) and two adenoid cystic carcinoma (ACC2 and ACCM) cell lines. We then used constructs that expressed antisense cDNAs to Id1 or Id2 to knockdown the expression of these proteins in cell lines where they were highly expressed, and determined the effects of the knockdown on cell proliferation, migration and invasion.
Id1 mRNA and protein were detectable in all cell lines, and expression of Id2 was variable, from absent to high. The ACC2 and ACCM cell lines expressed both Id1 and Id2, but Id1 was expressed at a higher level in the more aggressive ACCM cell line in comparison to ACC2 cells as confirmed by Id1 promoter-reporter assays. We therefore focused on the ACCM cells for the remainder of the study. We found that proliferation and invasiveness of ACCM cells were strongly reduced after Id1 knockdown whereas Id2 suppression had only a slight effect. Results of scratch and colony formation assays also confirmed that ACCM cell aggressiveness was significantly reduced upon Id1 knockdown. Finally, this knockdown resulted in reduced c-myc and enhanced cyclin-dependent kinase inhibitor p21 expression.
These results demonstrate that Id1 plays an important role in the control of human SGC cell aggressiveness and suggest a potential role as a marker of diagnosis, prognosis and progression of SGCs. Id1 suppression could represent a novel and effective approach for the treatment of salivary gland cancer.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Analysis</subject><subject>Cancer</subject><subject>Carcinoma, Adenoid Cystic - genetics</subject><subject>Carcinoma, Adenoid Cystic - metabolism</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - genetics</subject><subject>DNA binding proteins</subject><subject>Down-Regulation</subject><subject>Gene expression</subject><subject>Gene Knockdown Techniques</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Inhibitor of Differentiation Protein 1 - genetics</subject><subject>Inhibitor of Differentiation Protein 1 - metabolism</subject><subject>Inhibitor of Differentiation Protein 2 - genetics</subject><subject>Inhibitor of Differentiation Protein 2 - metabolism</subject><subject>Insulin</subject><subject>Membranes</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Regulation</subject><subject>Salivary Gland Neoplasms - genetics</subject><subject>Salivary Gland Neoplasms - metabolism</subject><subject>Salivary gland tumors</subject><subject>Transcription factors</subject><subject>Tumor Stem Cell Assay</subject><subject>Tumors</subject><subject>Up-Regulation</subject><issn>1471-2407</issn><issn>1471-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkl2L1DAUhoso7ofeeyUFQdaLrjlNkzY3C8vix8CCoOt1zKSnnSxpMibtoP_ehF2HGZFcJCd5zpu8J6coXgG5BOj4e2haqOqGtBXQChp4Upzut54erE-KsxjvCYG2I93z4qSmDFqg5LT4cafCiLNxY7nqoQzYLxpjuQ3emgGDmo13pXJ9adxOxRyYFI9jwBjNDsvNMilXRmXNToXf5Wgzq5XTGEqN1sYXxbNB2YgvH-fz4vvHD3c3n6vbL59WN9e3leZ1PVeCEFaLlqFA3aiOClivAQkHVffQs7rrek6haTgOpFW0Fw3VgoqWM1Izzdb0vLh60N0u6wl7jW4OysptMFN6mPTKyOMTZzZy9DtJORWEkiRw8SgQ_M8F4ywnE7MF5dAvUQJt2oYz2rYJffMPeu-X4JK9THEhmEjV3VOjsiiNG3y6V2dRec0SRjrgXaIu_0Ol0eNktHc4mLR_lPDuKCExM_6aR7XEKFffvh6zbw_YDSo7b6K3S_7VeAySB1AHH2PAYV84IDK3msy9JHMvJYspyPZeHxZ8n_C3t-gfsbnLEQ</recordid><startdate>20130322</startdate><enddate>20130322</enddate><creator>Sumida, Tomoki</creator><creator>Murase, Ryuichi</creator><creator>Onishi-Ishikawa, Akiko</creator><creator>McAllister, Sean D</creator><creator>Hamakawa, Hiroyuki</creator><creator>Desprez, Pierre-Yves</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130322</creationdate><title>Targeting Id1 reduces proliferation and invasion in aggressive human salivary gland cancer cells</title><author>Sumida, Tomoki ; Murase, Ryuichi ; Onishi-Ishikawa, Akiko ; McAllister, Sean D ; Hamakawa, Hiroyuki ; Desprez, Pierre-Yves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c622t-90052975e9ec4a8391bb1e061a2d1d5288d631446ef07a3d943c939765025c5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Analysis</topic><topic>Cancer</topic><topic>Carcinoma, Adenoid Cystic - genetics</topic><topic>Carcinoma, Adenoid Cystic - metabolism</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - genetics</topic><topic>DNA binding proteins</topic><topic>Down-Regulation</topic><topic>Gene expression</topic><topic>Gene Knockdown Techniques</topic><topic>Genotype & phenotype</topic><topic>Humans</topic><topic>Inhibitor of Differentiation Protein 1 - genetics</topic><topic>Inhibitor of Differentiation Protein 1 - metabolism</topic><topic>Inhibitor of Differentiation Protein 2 - genetics</topic><topic>Inhibitor of Differentiation Protein 2 - metabolism</topic><topic>Insulin</topic><topic>Membranes</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-myc - genetics</topic><topic>Regulation</topic><topic>Salivary Gland Neoplasms - genetics</topic><topic>Salivary Gland Neoplasms - metabolism</topic><topic>Salivary gland tumors</topic><topic>Transcription factors</topic><topic>Tumor Stem Cell Assay</topic><topic>Tumors</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sumida, Tomoki</creatorcontrib><creatorcontrib>Murase, Ryuichi</creatorcontrib><creatorcontrib>Onishi-Ishikawa, Akiko</creatorcontrib><creatorcontrib>McAllister, Sean D</creatorcontrib><creatorcontrib>Hamakawa, Hiroyuki</creatorcontrib><creatorcontrib>Desprez, Pierre-Yves</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sumida, Tomoki</au><au>Murase, Ryuichi</au><au>Onishi-Ishikawa, Akiko</au><au>McAllister, Sean D</au><au>Hamakawa, Hiroyuki</au><au>Desprez, Pierre-Yves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeting Id1 reduces proliferation and invasion in aggressive human salivary gland cancer cells</atitle><jtitle>BMC cancer</jtitle><addtitle>BMC Cancer</addtitle><date>2013-03-22</date><risdate>2013</risdate><volume>13</volume><issue>1</issue><spage>141</spage><epage>141</epage><pages>141-141</pages><artnum>141</artnum><issn>1471-2407</issn><eissn>1471-2407</eissn><abstract>Salivary gland cancer (SGC) is one of the common malignancies of the head and neck area. It develops in the minor and major salivary glands and sometimes metastasizes to other organs, particularly to the lungs. Inhibitors of differentiation (Id) proteins are negative regulators of basic helix-loop-helix transcription factors that control malignant cell behavior and tumor aggressiveness in many tissues. In this study, our goal was to determine the potential role of Id proteins, particularly Id1, during human SGC cell progression.
We first determined the expression levels of Id1 and Id2 in four SGC cell lines: two adenocarcinoma of the salivary gland (HSG and HSY) and two adenoid cystic carcinoma (ACC2 and ACCM) cell lines. We then used constructs that expressed antisense cDNAs to Id1 or Id2 to knockdown the expression of these proteins in cell lines where they were highly expressed, and determined the effects of the knockdown on cell proliferation, migration and invasion.
Id1 mRNA and protein were detectable in all cell lines, and expression of Id2 was variable, from absent to high. The ACC2 and ACCM cell lines expressed both Id1 and Id2, but Id1 was expressed at a higher level in the more aggressive ACCM cell line in comparison to ACC2 cells as confirmed by Id1 promoter-reporter assays. We therefore focused on the ACCM cells for the remainder of the study. We found that proliferation and invasiveness of ACCM cells were strongly reduced after Id1 knockdown whereas Id2 suppression had only a slight effect. Results of scratch and colony formation assays also confirmed that ACCM cell aggressiveness was significantly reduced upon Id1 knockdown. Finally, this knockdown resulted in reduced c-myc and enhanced cyclin-dependent kinase inhibitor p21 expression.
These results demonstrate that Id1 plays an important role in the control of human SGC cell aggressiveness and suggest a potential role as a marker of diagnosis, prognosis and progression of SGCs. Id1 suppression could represent a novel and effective approach for the treatment of salivary gland cancer.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>23517130</pmid><doi>10.1186/1471-2407-13-141</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - metabolism Analysis Cancer Carcinoma, Adenoid Cystic - genetics Carcinoma, Adenoid Cystic - metabolism Cell adhesion & migration Cell culture Cell cycle Cell Line, Tumor Cell Movement Cell Proliferation Cyclin-Dependent Kinase Inhibitor p21 - genetics DNA binding proteins Down-Regulation Gene expression Gene Knockdown Techniques Genotype & phenotype Humans Inhibitor of Differentiation Protein 1 - genetics Inhibitor of Differentiation Protein 1 - metabolism Inhibitor of Differentiation Protein 2 - genetics Inhibitor of Differentiation Protein 2 - metabolism Insulin Membranes Physiological aspects Proteins Proto-Oncogene Proteins c-myc - genetics Regulation Salivary Gland Neoplasms - genetics Salivary Gland Neoplasms - metabolism Salivary gland tumors Transcription factors Tumor Stem Cell Assay Tumors Up-Regulation |
| title | Targeting Id1 reduces proliferation and invasion in aggressive human salivary gland cancer cells |
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