CD34 Promotes Satellite Cell Motility and Entry into Proliferation to Facilitate Efficient Skeletal Muscle Regeneration
Expression of the cell surface sialomucin CD34 is common to many adult stem cell types, including muscle satellite cells. However, no clear stem cell or regeneration‐related phenotype has ever been reported in mice lacking CD34, and its function on these cells remains poorly understood. Here, we ass...
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| Veröffentlicht in: | Stem cells (Dayton, Ohio) Ohio), 2011-12, Vol.29 (12), p.2030-2041 |
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| creator | Alfaro, Leslie Ann So Dick, Sarah A. Siegel, Ashley L. Anonuevo, Adam S. McNagny, Kelly M. Megeney, Lynn A. Cornelison, D.D.W. Rossi, Fabio M.V. |
| description | Expression of the cell surface sialomucin CD34 is common to many adult stem cell types, including muscle satellite cells. However, no clear stem cell or regeneration‐related phenotype has ever been reported in mice lacking CD34, and its function on these cells remains poorly understood. Here, we assess the functional role of CD34 on satellite cell‐mediated muscle regeneration. We show that Cd34−/− mice, which have no obvious developmental phenotype, display a defect in muscle regeneration when challenged with either acute or chronic muscle injury. This regenerative defect is caused by impaired entry into proliferation and delayed myogenic progression. Consistent with the reported antiadhesive function of CD34, knockout satellite cells also show decreased motility along their host myofiber. Altogether, our results identify a role for CD34 in the poorly understood early steps of satellite cell activation and provide the first evidence that beyond being a stem cell marker, CD34 may play an important function in modulating stem cell activity. STEM Cells 2011;29:2030–2041. |
| doi_str_mv | 10.1002/stem.759 |
| format | Article |
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However, no clear stem cell or regeneration‐related phenotype has ever been reported in mice lacking CD34, and its function on these cells remains poorly understood. Here, we assess the functional role of CD34 on satellite cell‐mediated muscle regeneration. We show that Cd34−/− mice, which have no obvious developmental phenotype, display a defect in muscle regeneration when challenged with either acute or chronic muscle injury. This regenerative defect is caused by impaired entry into proliferation and delayed myogenic progression. Consistent with the reported antiadhesive function of CD34, knockout satellite cells also show decreased motility along their host myofiber. Altogether, our results identify a role for CD34 in the poorly understood early steps of satellite cell activation and provide the first evidence that beyond being a stem cell marker, CD34 may play an important function in modulating stem cell activity. STEM Cells 2011;29:2030–2041.</description><identifier>ISSN: 1066-5099</identifier><identifier>EISSN: 1549-4918</identifier><identifier>DOI: 10.1002/stem.759</identifier><identifier>PMID: 21997891</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Antigens, CD34 - genetics ; Antigens, CD34 - metabolism ; CD34 ; Cell Movement ; Cell Proliferation ; Elapid Venoms - adverse effects ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microscopy, Confocal ; Muscle regeneration ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - injuries ; Muscle, Skeletal - physiology ; Point Mutation ; Regeneration ; Satellite cell activation ; Satellite Cells, Skeletal Muscle - cytology ; Satellite Cells, Skeletal Muscle - physiology ; Time-Lapse Imaging</subject><ispartof>Stem cells (Dayton, Ohio), 2011-12, Vol.29 (12), p.2030-2041</ispartof><rights>Copyright © 2011 AlphaMed Press</rights><rights>Copyright © 2011 AlphaMed Press.</rights><rights>AlphaMed Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4429-b8ec547523709bee376175f608bb9d2f61f3446256ba770ac6bc07c0d3eeeb083</citedby><cites>FETCH-LOGICAL-c4429-b8ec547523709bee376175f608bb9d2f61f3446256ba770ac6bc07c0d3eeeb083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21997891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alfaro, Leslie Ann So</creatorcontrib><creatorcontrib>Dick, Sarah A.</creatorcontrib><creatorcontrib>Siegel, Ashley L.</creatorcontrib><creatorcontrib>Anonuevo, Adam S.</creatorcontrib><creatorcontrib>McNagny, Kelly M.</creatorcontrib><creatorcontrib>Megeney, Lynn A.</creatorcontrib><creatorcontrib>Cornelison, D.D.W.</creatorcontrib><creatorcontrib>Rossi, Fabio M.V.</creatorcontrib><title>CD34 Promotes Satellite Cell Motility and Entry into Proliferation to Facilitate Efficient Skeletal Muscle Regeneration</title><title>Stem cells (Dayton, Ohio)</title><addtitle>Stem Cells</addtitle><description>Expression of the cell surface sialomucin CD34 is common to many adult stem cell types, including muscle satellite cells. However, no clear stem cell or regeneration‐related phenotype has ever been reported in mice lacking CD34, and its function on these cells remains poorly understood. Here, we assess the functional role of CD34 on satellite cell‐mediated muscle regeneration. We show that Cd34−/− mice, which have no obvious developmental phenotype, display a defect in muscle regeneration when challenged with either acute or chronic muscle injury. This regenerative defect is caused by impaired entry into proliferation and delayed myogenic progression. Consistent with the reported antiadhesive function of CD34, knockout satellite cells also show decreased motility along their host myofiber. Altogether, our results identify a role for CD34 in the poorly understood early steps of satellite cell activation and provide the first evidence that beyond being a stem cell marker, CD34 may play an important function in modulating stem cell activity. STEM Cells 2011;29:2030–2041.</description><subject>Animals</subject><subject>Antigens, CD34 - genetics</subject><subject>Antigens, CD34 - metabolism</subject><subject>CD34</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Elapid Venoms - adverse effects</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Microscopy, Confocal</subject><subject>Muscle regeneration</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - injuries</subject><subject>Muscle, Skeletal - physiology</subject><subject>Point Mutation</subject><subject>Regeneration</subject><subject>Satellite cell activation</subject><subject>Satellite Cells, Skeletal Muscle - cytology</subject><subject>Satellite Cells, Skeletal Muscle - physiology</subject><subject>Time-Lapse Imaging</subject><issn>1066-5099</issn><issn>1549-4918</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtP3DAURi3Uilcr8QsqL9mE-hU73iBVwwxUAoE6dG05nhtwSWJqe0Dz73HElJZFV9ePc4-v_CF0RMkJJYR9TRmGE1XrHbRPa6EroWnzoayJlFVNtN5DByn9IoSKuml20R6jWqtG0330PDvjAt_EMIQMCS9thr73GfCsVHwVsi-7DbbjCs_HHDfYjzlMfO87iDb7MOJysLBuAks3nneddx7GjJcP0EO2RbNOrgf8A-5g3DZ9Qh872yf4vK2H6Odifju7qC6vz7_Pvl1WTgimq7YBVwtVM66IbgG4klTVnSRN2-oV6yTtuBCS1bK1ShHrZOuIcmTFAaAlDT9Ep6_ex3U7wMqVuaLtzWP0g40bE6w3729Gf2_uwpPhkjdK8yI43gpi-L2GlM3gkyufY0cI62So4IIwRij7i7oYUorQvT1DiZlyMlNOpuRU0C__jvUG_gmmANUr8Ox72PxXZJa386tJ-AIS45-U</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Alfaro, Leslie Ann So</creator><creator>Dick, Sarah A.</creator><creator>Siegel, Ashley L.</creator><creator>Anonuevo, Adam S.</creator><creator>McNagny, Kelly M.</creator><creator>Megeney, Lynn A.</creator><creator>Cornelison, D.D.W.</creator><creator>Rossi, Fabio M.V.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>201112</creationdate><title>CD34 Promotes Satellite Cell Motility and Entry into Proliferation to Facilitate Efficient Skeletal Muscle Regeneration</title><author>Alfaro, Leslie Ann So ; Dick, Sarah A. ; Siegel, Ashley L. ; Anonuevo, Adam S. ; McNagny, Kelly M. ; Megeney, Lynn A. ; Cornelison, D.D.W. ; Rossi, Fabio M.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4429-b8ec547523709bee376175f608bb9d2f61f3446256ba770ac6bc07c0d3eeeb083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antigens, CD34 - genetics</topic><topic>Antigens, CD34 - metabolism</topic><topic>CD34</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Elapid Venoms - adverse effects</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Microscopy, Confocal</topic><topic>Muscle regeneration</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - injuries</topic><topic>Muscle, Skeletal - physiology</topic><topic>Point Mutation</topic><topic>Regeneration</topic><topic>Satellite cell activation</topic><topic>Satellite Cells, Skeletal Muscle - cytology</topic><topic>Satellite Cells, Skeletal Muscle - physiology</topic><topic>Time-Lapse Imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alfaro, Leslie Ann So</creatorcontrib><creatorcontrib>Dick, Sarah A.</creatorcontrib><creatorcontrib>Siegel, Ashley L.</creatorcontrib><creatorcontrib>Anonuevo, Adam S.</creatorcontrib><creatorcontrib>McNagny, Kelly M.</creatorcontrib><creatorcontrib>Megeney, Lynn A.</creatorcontrib><creatorcontrib>Cornelison, D.D.W.</creatorcontrib><creatorcontrib>Rossi, Fabio M.V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cells (Dayton, Ohio)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alfaro, Leslie Ann So</au><au>Dick, Sarah A.</au><au>Siegel, Ashley L.</au><au>Anonuevo, Adam S.</au><au>McNagny, Kelly M.</au><au>Megeney, Lynn A.</au><au>Cornelison, D.D.W.</au><au>Rossi, Fabio M.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD34 Promotes Satellite Cell Motility and Entry into Proliferation to Facilitate Efficient Skeletal Muscle Regeneration</atitle><jtitle>Stem cells (Dayton, Ohio)</jtitle><addtitle>Stem Cells</addtitle><date>2011-12</date><risdate>2011</risdate><volume>29</volume><issue>12</issue><spage>2030</spage><epage>2041</epage><pages>2030-2041</pages><issn>1066-5099</issn><eissn>1549-4918</eissn><abstract>Expression of the cell surface sialomucin CD34 is common to many adult stem cell types, including muscle satellite cells. However, no clear stem cell or regeneration‐related phenotype has ever been reported in mice lacking CD34, and its function on these cells remains poorly understood. Here, we assess the functional role of CD34 on satellite cell‐mediated muscle regeneration. We show that Cd34−/− mice, which have no obvious developmental phenotype, display a defect in muscle regeneration when challenged with either acute or chronic muscle injury. This regenerative defect is caused by impaired entry into proliferation and delayed myogenic progression. Consistent with the reported antiadhesive function of CD34, knockout satellite cells also show decreased motility along their host myofiber. Altogether, our results identify a role for CD34 in the poorly understood early steps of satellite cell activation and provide the first evidence that beyond being a stem cell marker, CD34 may play an important function in modulating stem cell activity. 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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antigens, CD34 - genetics Antigens, CD34 - metabolism CD34 Cell Movement Cell Proliferation Elapid Venoms - adverse effects Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Confocal Muscle regeneration Muscle, Skeletal - drug effects Muscle, Skeletal - injuries Muscle, Skeletal - physiology Point Mutation Regeneration Satellite cell activation Satellite Cells, Skeletal Muscle - cytology Satellite Cells, Skeletal Muscle - physiology Time-Lapse Imaging |
| title | CD34 Promotes Satellite Cell Motility and Entry into Proliferation to Facilitate Efficient Skeletal Muscle Regeneration |
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