Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation
The bovine immunodeficiency virus (BIV) transactivator (BTat) recruits the bovine cyclin T1 (B-cyclin T1) to the LTR to facilitate the transcription of BIV. Here, we demonstrate that bovine hexamethylene bisacetamide (HMBA)-induced protein 1 (BHEXIM1) inhibits BTat-mediated BIV LTR transcription. Th...
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| Veröffentlicht in: | Veterinary research (Paris) 2013-03, Vol.44 (1), p.21-21, Article 21 |
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| creator | Guo, Hong-yan Ma, Yong-gang Gai, Yuan-ming Liang, Zhi-bin Ma, Jing Su, Yang Zhang, Qi-cheng Chen, Qi-min Tan, Juan |
| description | The bovine immunodeficiency virus (BIV) transactivator (BTat) recruits the bovine cyclin T1 (B-cyclin T1) to the LTR to facilitate the transcription of BIV. Here, we demonstrate that bovine hexamethylene bisacetamide (HMBA)-induced protein 1 (BHEXIM1) inhibits BTat-mediated BIV LTR transcription. The results of in vivo and in vitro assays show direct binding of BHEXIM1 to the B-cyclin T1. These results suggest that the repression arises from BHEXIM1-BTat competition for B-cyclin T1, which allows BHEXIM1 to displace BTat from B-cyclin T1. Furthermore, we found that the C-terminal region and the centrally located region of BHEXIM1 are required for BHEXIM1 to associate with B-cyclin T1. Knockdown of BHEXIM1 enhances BIV replication. Taken together, our study provides the first clear evidence that BHEXIM1 is involved in BIV replication through regulating BTat-mediated transactivation. |
| doi_str_mv | 10.1186/1297-9716-44-21 |
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Here, we demonstrate that bovine hexamethylene bisacetamide (HMBA)-induced protein 1 (BHEXIM1) inhibits BTat-mediated BIV LTR transcription. The results of in vivo and in vitro assays show direct binding of BHEXIM1 to the B-cyclin T1. These results suggest that the repression arises from BHEXIM1-BTat competition for B-cyclin T1, which allows BHEXIM1 to displace BTat from B-cyclin T1. Furthermore, we found that the C-terminal region and the centrally located region of BHEXIM1 are required for BHEXIM1 to associate with B-cyclin T1. Knockdown of BHEXIM1 enhances BIV replication. Taken together, our study provides the first clear evidence that BHEXIM1 is involved in BIV replication through regulating BTat-mediated transactivation.</description><identifier>ISSN: 1297-9716</identifier><identifier>ISSN: 0928-4249</identifier><identifier>EISSN: 1297-9716</identifier><identifier>DOI: 10.1186/1297-9716-44-21</identifier><identifier>PMID: 23537346</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Cattle ; Cyclin T - genetics ; Cyclin T - metabolism ; Cyclohexane ; Escherichia coli ; Immunodeficiency Virus, Bovine - metabolism ; Plasmids ; Polymerase Chain Reaction - veterinary ; Positive Transcriptional Elongation Factor B - genetics ; Positive Transcriptional Elongation Factor B - metabolism ; Retroviridae Proteins - metabolism ; Terminal Repeat Sequences ; Transcriptional Activation</subject><ispartof>Veterinary research (Paris), 2013-03, Vol.44 (1), p.21-21, Article 21</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>Copyright © 2013 Guo et al.; licensee BioMed Central Ltd. 2013 Guo et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b519t-e6b02df1f5b18f43432f9aede84b87e48ede1d772c591ca54943dee2b3e44d053</citedby><cites>FETCH-LOGICAL-b519t-e6b02df1f5b18f43432f9aede84b87e48ede1d772c591ca54943dee2b3e44d053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630055/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630055/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23537346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Hong-yan</creatorcontrib><creatorcontrib>Ma, Yong-gang</creatorcontrib><creatorcontrib>Gai, Yuan-ming</creatorcontrib><creatorcontrib>Liang, Zhi-bin</creatorcontrib><creatorcontrib>Ma, Jing</creatorcontrib><creatorcontrib>Su, Yang</creatorcontrib><creatorcontrib>Zhang, Qi-cheng</creatorcontrib><creatorcontrib>Chen, Qi-min</creatorcontrib><creatorcontrib>Tan, Juan</creatorcontrib><title>Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation</title><title>Veterinary research (Paris)</title><addtitle>Vet Res</addtitle><description>The bovine immunodeficiency virus (BIV) transactivator (BTat) recruits the bovine cyclin T1 (B-cyclin T1) to the LTR to facilitate the transcription of BIV. Here, we demonstrate that bovine hexamethylene bisacetamide (HMBA)-induced protein 1 (BHEXIM1) inhibits BTat-mediated BIV LTR transcription. The results of in vivo and in vitro assays show direct binding of BHEXIM1 to the B-cyclin T1. These results suggest that the repression arises from BHEXIM1-BTat competition for B-cyclin T1, which allows BHEXIM1 to displace BTat from B-cyclin T1. Furthermore, we found that the C-terminal region and the centrally located region of BHEXIM1 are required for BHEXIM1 to associate with B-cyclin T1. Knockdown of BHEXIM1 enhances BIV replication. Taken together, our study provides the first clear evidence that BHEXIM1 is involved in BIV replication through regulating BTat-mediated transactivation.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cyclin T - genetics</subject><subject>Cyclin T - metabolism</subject><subject>Cyclohexane</subject><subject>Escherichia coli</subject><subject>Immunodeficiency Virus, Bovine - metabolism</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Positive Transcriptional Elongation Factor B - genetics</subject><subject>Positive Transcriptional Elongation Factor B - metabolism</subject><subject>Retroviridae Proteins - metabolism</subject><subject>Terminal Repeat Sequences</subject><subject>Transcriptional Activation</subject><issn>1297-9716</issn><issn>0928-4249</issn><issn>1297-9716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1Uk1P3DAQjapWhVLOvVWReuESsGM7H5dKLKKABOJCJW6WY0-yUyX2Yicr8e_rNLBiJSofPHrz5un5eZLkGyWnlFbFGc3rMqtLWmScZzn9kBzukI9v6oPkSwh_CKEFE_xzcpAzwUrGi8PkaeW2aCG9vny8uaMp2jU2OIa0WWAchsk6Ay1qBKuf0y36KaQeNj1qNaKz6bj2burWEeumPkK2S1cPaswGMKhGMOnolQ1Kj7j9N_A1-dSqPsDxy32U_P51-XBxnd3eX91cnN9mjaD1mEHRkNy0tBUNrVrOOMvbWoGBijdVCbyKJTVlmWtRU60ErzkzAHnDgHNDBDtKfi66m6mJXjTYaKSXG4-D8s_SKZT7HYtr2bmtZAUjRMwCq0WgQfcfgf2OdoOcI5dz5JJzmdMocvLiwrunCcIoBwwa-l5ZcFOQlDFKalIWM_XHQu1UDxJt66KqnunyXDBe0ZqwOrJO32HFY2BA7Wz8qojvDZwtA9q7EDy0uxdQIuclesfz97fJ7fivW8P-AhthxRc</recordid><startdate>20130327</startdate><enddate>20130327</enddate><creator>Guo, Hong-yan</creator><creator>Ma, Yong-gang</creator><creator>Gai, Yuan-ming</creator><creator>Liang, Zhi-bin</creator><creator>Ma, Jing</creator><creator>Su, Yang</creator><creator>Zhang, Qi-cheng</creator><creator>Chen, Qi-min</creator><creator>Tan, Juan</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130327</creationdate><title>Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation</title><author>Guo, Hong-yan ; Ma, Yong-gang ; Gai, Yuan-ming ; Liang, Zhi-bin ; Ma, Jing ; Su, Yang ; Zhang, Qi-cheng ; Chen, Qi-min ; Tan, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b519t-e6b02df1f5b18f43432f9aede84b87e48ede1d772c591ca54943dee2b3e44d053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cyclin T - genetics</topic><topic>Cyclin T - metabolism</topic><topic>Cyclohexane</topic><topic>Escherichia coli</topic><topic>Immunodeficiency Virus, Bovine - metabolism</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Positive Transcriptional Elongation Factor B - genetics</topic><topic>Positive Transcriptional Elongation Factor B - metabolism</topic><topic>Retroviridae Proteins - metabolism</topic><topic>Terminal Repeat Sequences</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Hong-yan</creatorcontrib><creatorcontrib>Ma, Yong-gang</creatorcontrib><creatorcontrib>Gai, Yuan-ming</creatorcontrib><creatorcontrib>Liang, Zhi-bin</creatorcontrib><creatorcontrib>Ma, Jing</creatorcontrib><creatorcontrib>Su, Yang</creatorcontrib><creatorcontrib>Zhang, Qi-cheng</creatorcontrib><creatorcontrib>Chen, Qi-min</creatorcontrib><creatorcontrib>Tan, Juan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Veterinary research (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Hong-yan</au><au>Ma, Yong-gang</au><au>Gai, Yuan-ming</au><au>Liang, Zhi-bin</au><au>Ma, Jing</au><au>Su, Yang</au><au>Zhang, Qi-cheng</au><au>Chen, Qi-min</au><au>Tan, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation</atitle><jtitle>Veterinary research (Paris)</jtitle><addtitle>Vet Res</addtitle><date>2013-03-27</date><risdate>2013</risdate><volume>44</volume><issue>1</issue><spage>21</spage><epage>21</epage><pages>21-21</pages><artnum>21</artnum><issn>1297-9716</issn><issn>0928-4249</issn><eissn>1297-9716</eissn><abstract>The bovine immunodeficiency virus (BIV) transactivator (BTat) recruits the bovine cyclin T1 (B-cyclin T1) to the LTR to facilitate the transcription of BIV. Here, we demonstrate that bovine hexamethylene bisacetamide (HMBA)-induced protein 1 (BHEXIM1) inhibits BTat-mediated BIV LTR transcription. The results of in vivo and in vitro assays show direct binding of BHEXIM1 to the B-cyclin T1. These results suggest that the repression arises from BHEXIM1-BTat competition for B-cyclin T1, which allows BHEXIM1 to displace BTat from B-cyclin T1. Furthermore, we found that the C-terminal region and the centrally located region of BHEXIM1 are required for BHEXIM1 to associate with B-cyclin T1. Knockdown of BHEXIM1 enhances BIV replication. Taken together, our study provides the first clear evidence that BHEXIM1 is involved in BIV replication through regulating BTat-mediated transactivation.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>23537346</pmid><doi>10.1186/1297-9716-44-21</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cattle Cyclin T - genetics Cyclin T - metabolism Cyclohexane Escherichia coli Immunodeficiency Virus, Bovine - metabolism Plasmids Polymerase Chain Reaction - veterinary Positive Transcriptional Elongation Factor B - genetics Positive Transcriptional Elongation Factor B - metabolism Retroviridae Proteins - metabolism Terminal Repeat Sequences Transcriptional Activation |
| title | Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation |
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