Phyllanthus emblica L. Enhances Human Umbilical Vein Endothelial Wound Healing and Sprouting
Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constitu...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-9 |
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| creator | Chularojmontri, Linda Suwatronnakorn, Maneewan Wattanapitayakul, Suvara K. |
| description | Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constituents and capacity of Phyllanthus emblica L. (PE) fruit in freeze-dried power form. The pharmacological properties of PE were investigated using human umbilical vein endothelial cells (HUVECs) in the aspects of endothelial cell proliferation, nitric oxide (NO) production, wound healing, cell migration, in vitro angiogenesis, and VEGF gene expression. The ASC content of PE was 1.574% + 0.046% (w/w) as determined by HPLC and the total phenolic content was 36.1% ± 0.7% gallic acid equivalent when measured by Folin-Ciocalteu assay. The FRAP assay revealed a relatively high antioxidant capacity at 3,643 + 192.5 µmole/mg. PE at 0.1 to 10 µg/mL did not significantly influence endothelial cell proliferation, but at higher concentrations PE decreased cell survival to 62%. PE significantly promoted NO production, endothelial wound closure, endothelial sprouting, and VEGF mRNA expression. Therefore, PE is a candidate for antioxidant supplement that promotes endothelial function and restores wound healing competency. |
| doi_str_mv | 10.1155/2013/720728 |
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Enhances Human Umbilical Vein Endothelial Wound Healing and Sprouting</title><source>PubMed Central Open Access</source><source>Wiley Online Library Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Chularojmontri, Linda ; Suwatronnakorn, Maneewan ; Wattanapitayakul, Suvara K.</creator><contributor>Beerhues, Ludger ; Ludger Beerhues</contributor><creatorcontrib>Chularojmontri, Linda ; Suwatronnakorn, Maneewan ; Wattanapitayakul, Suvara K. ; Beerhues, Ludger ; Ludger Beerhues</creatorcontrib><description>Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constituents and capacity of Phyllanthus emblica L. (PE) fruit in freeze-dried power form. The pharmacological properties of PE were investigated using human umbilical vein endothelial cells (HUVECs) in the aspects of endothelial cell proliferation, nitric oxide (NO) production, wound healing, cell migration, in vitro angiogenesis, and VEGF gene expression. The ASC content of PE was 1.574% + 0.046% (w/w) as determined by HPLC and the total phenolic content was 36.1% ± 0.7% gallic acid equivalent when measured by Folin-Ciocalteu assay. The FRAP assay revealed a relatively high antioxidant capacity at 3,643 + 192.5 µmole/mg. PE at 0.1 to 10 µg/mL did not significantly influence endothelial cell proliferation, but at higher concentrations PE decreased cell survival to 62%. PE significantly promoted NO production, endothelial wound closure, endothelial sprouting, and VEGF mRNA expression. Therefore, PE is a candidate for antioxidant supplement that promotes endothelial function and restores wound healing competency.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2013/720728</identifier><identifier>PMID: 23606890</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Acids ; Alternative medicine ; Angiogenesis ; Antioxidants ; Apoptosis ; Atherosclerosis ; Cancer ; Cardiovascular diseases ; Cell adhesion & migration ; Cell migration ; Cell proliferation ; Cell survival ; Cytotoxicity ; Drug dosages ; Endothelial cells ; Gallic acid ; Gene expression ; Health risks ; High-performance liquid chromatography ; Hypoxia ; Liquid chromatography ; Medical research ; Nitric oxide ; Oxidative stress ; Phenolic compounds ; Phenols ; Phyllanthus emblica ; Reactive oxygen species ; Umbilical vein ; Vascular endothelial growth factor ; Veins & arteries ; Wound healing</subject><ispartof>Evidence-based complementary and alternative medicine, 2013-01, Vol.2013 (2013), p.1-9</ispartof><rights>Copyright © 2013 Linda Chularojmontri et al.</rights><rights>Copyright © 2013 Linda Chularojmontri et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2013 Linda Chularojmontri et al. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-2b8d5b274045f0482acd2e643199a1d11d9b4ef119fca933a4ba04eb1f0e652e3</citedby><cites>FETCH-LOGICAL-c429t-2b8d5b274045f0482acd2e643199a1d11d9b4ef119fca933a4ba04eb1f0e652e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626238/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626238/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23606890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Beerhues, Ludger</contributor><contributor>Ludger Beerhues</contributor><creatorcontrib>Chularojmontri, Linda</creatorcontrib><creatorcontrib>Suwatronnakorn, Maneewan</creatorcontrib><creatorcontrib>Wattanapitayakul, Suvara K.</creatorcontrib><title>Phyllanthus emblica L. Enhances Human Umbilical Vein Endothelial Wound Healing and Sprouting</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constituents and capacity of Phyllanthus emblica L. (PE) fruit in freeze-dried power form. The pharmacological properties of PE were investigated using human umbilical vein endothelial cells (HUVECs) in the aspects of endothelial cell proliferation, nitric oxide (NO) production, wound healing, cell migration, in vitro angiogenesis, and VEGF gene expression. The ASC content of PE was 1.574% + 0.046% (w/w) as determined by HPLC and the total phenolic content was 36.1% ± 0.7% gallic acid equivalent when measured by Folin-Ciocalteu assay. The FRAP assay revealed a relatively high antioxidant capacity at 3,643 + 192.5 µmole/mg. PE at 0.1 to 10 µg/mL did not significantly influence endothelial cell proliferation, but at higher concentrations PE decreased cell survival to 62%. PE significantly promoted NO production, endothelial wound closure, endothelial sprouting, and VEGF mRNA expression. Therefore, PE is a candidate for antioxidant supplement that promotes endothelial function and restores wound healing competency.</description><subject>Acids</subject><subject>Alternative medicine</subject><subject>Angiogenesis</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Atherosclerosis</subject><subject>Cancer</subject><subject>Cardiovascular diseases</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Cell survival</subject><subject>Cytotoxicity</subject><subject>Drug dosages</subject><subject>Endothelial cells</subject><subject>Gallic acid</subject><subject>Gene expression</subject><subject>Health risks</subject><subject>High-performance liquid chromatography</subject><subject>Hypoxia</subject><subject>Liquid chromatography</subject><subject>Medical research</subject><subject>Nitric oxide</subject><subject>Oxidative stress</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Phyllanthus emblica</subject><subject>Reactive oxygen species</subject><subject>Umbilical vein</subject><subject>Vascular endothelial growth factor</subject><subject>Veins & arteries</subject><subject>Wound healing</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkcFrFTEQxoNYbK2ePCsLXkR5bTLJbjYXQUr1FR4oaNWDEGZ3Z7spu8lzs6v0v28eWx_Vi6eZyffjYyYfY88EPxEiz0-BC3mqgWsoH7AjoZVYKSjLh_tefz9kj2O85hyM1voROwRZ8KI0_Ij9-NTd9D36qZtjRkPVuxqzzUl27jv0NcVsPQ_os8uhcjupz76S80ltwtRR79LDtzD7JlsT9s5fZZj6z9sxzFOanrCDFvtIT-_qMbt8f_7lbL3afPxwcfZus6oVmGkFVdnkFWjFVd5yVQLWDVChpDAGRSNEYypFrRCmrdFIiapCrqgSLaciB5LH7O3iu52rgZqa_DRib7ejG3C8sQGd_VvxrrNX4ZeVBRQgy2Tw6s5gDD9nipMdXKxp9zEU5miFTJCBHIqEvvwHvQ7z6NN5NkUgoTQCTKLeLFQ9hhhHavfLCG53qdldanZJLdEv7u-_Z__ElIDXC9A53-Bv9x-35wtMCaEW97AyErSQt1RXqLg</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Chularojmontri, Linda</creator><creator>Suwatronnakorn, Maneewan</creator><creator>Wattanapitayakul, Suvara K.</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130101</creationdate><title>Phyllanthus emblica L. 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Enhances Human Umbilical Vein Endothelial Wound Healing and Sprouting</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>2013</volume><issue>2013</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constituents and capacity of Phyllanthus emblica L. (PE) fruit in freeze-dried power form. The pharmacological properties of PE were investigated using human umbilical vein endothelial cells (HUVECs) in the aspects of endothelial cell proliferation, nitric oxide (NO) production, wound healing, cell migration, in vitro angiogenesis, and VEGF gene expression. The ASC content of PE was 1.574% + 0.046% (w/w) as determined by HPLC and the total phenolic content was 36.1% ± 0.7% gallic acid equivalent when measured by Folin-Ciocalteu assay. The FRAP assay revealed a relatively high antioxidant capacity at 3,643 + 192.5 µmole/mg. PE at 0.1 to 10 µg/mL did not significantly influence endothelial cell proliferation, but at higher concentrations PE decreased cell survival to 62%. PE significantly promoted NO production, endothelial wound closure, endothelial sprouting, and VEGF mRNA expression. Therefore, PE is a candidate for antioxidant supplement that promotes endothelial function and restores wound healing competency.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>23606890</pmid><doi>10.1155/2013/720728</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Alternative medicine Angiogenesis Antioxidants Apoptosis Atherosclerosis Cancer Cardiovascular diseases Cell adhesion & migration Cell migration Cell proliferation Cell survival Cytotoxicity Drug dosages Endothelial cells Gallic acid Gene expression Health risks High-performance liquid chromatography Hypoxia Liquid chromatography Medical research Nitric oxide Oxidative stress Phenolic compounds Phenols Phyllanthus emblica Reactive oxygen species Umbilical vein Vascular endothelial growth factor Veins & arteries Wound healing |
| title | Phyllanthus emblica L. Enhances Human Umbilical Vein Endothelial Wound Healing and Sprouting |
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