Identification of Regions of Rabbit Muscle Pyruvate Kinase Important for Allosteric Regulation by Phenylalanine, Detected by H/D Exchange Mass Spectrometry

Mass spectrometry has been used to determine the number of exchangeable backbone amide protons and the associated rate constants that are altered when rabbit muscle pyruvate kinase (rM1-PYK) binds either the allosteric inhibitor (phenylalanine) or a nonallosteric analogue of the inhibitor. Alanine i...

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Veröffentlicht in:	Biochemistry (Easton) 2013-03, Vol.52 (11), p.1998-2006
Hauptverfasser:	Prasannan, Charulata B, Villar, Maria T, Artigues, Antonio, Fenton, Aron W
Format:	Artikel
Sprache:	eng
Schlagworte:	alanine Allosteric Regulation Allosteric Site Animals experimental design Mass Spectrometry Models, Molecular muscles Muscles - chemistry Muscles - enzymology Muscles - metabolism mutagenesis peptides Peptides - chemistry Peptides - metabolism phenylalanine Phenylalanine - metabolism Protein Binding Protein Conformation protons pyruvate kinase Pyruvate Kinase - chemistry Pyruvate Kinase - metabolism Rabbits
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