Surface-patterned electrode bioreactor for electrical stimulation
We present a microscale cell culture system with an interdigitated microarray of excimer-laser-ablated indium tin oxide electrodes for electrical stimulation of cultured cells. The system has been characterized in a range of geometeries and stimulation regimes via electrochemical impedance spectrosc...
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| Veröffentlicht in: | Lab on a chip 2010-01, Vol.10 (6), p.692-700 |
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| container_issue | 6 |
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| container_title | Lab on a chip |
| container_volume | 10 |
| creator | Tandon, Nina Marsano, Anna Maidhof, Robert Numata, Keiji Montouri-Sorrentino, Chrystina Cannizzaro, Christopher Voldman, Joel Vunjak-Novakovic, Gordana |
| description | We present a microscale cell culture system with an interdigitated microarray of excimer-laser-ablated indium tin oxide electrodes for electrical stimulation of cultured cells. The system has been characterized in a range of geometeries and stimulation regimes via electrochemical impedance spectroscopy and used to culture primary cardiomyocytes and human adipose derived stem cells. Over 6 days of culture with electrical stimulation (2 ms duration, 1 Hz, 180 microm wide electrodes with 200 microm spacing), both cell types exhibited enhanced proliferation, elongation and alignment, and adipose derived stem cells exhibited higher numbers of Connexin-43-composed gap junctions. |
| doi_str_mv | 10.1039/b917743d |
| format | Article |
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The system has been characterized in a range of geometeries and stimulation regimes via electrochemical impedance spectroscopy and used to culture primary cardiomyocytes and human adipose derived stem cells. 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| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2010-01, Vol.10 (6), p.692-700 |
| issn | 1473-0197 1473-0189 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Animals Bioreactors Cell Culture Techniques - instrumentation Cells, Cultured Electric Stimulation - instrumentation Electrodes Equipment Design Equipment Failure Analysis Microfluidics - instrumentation Myocytes, Cardiac - physiology Rats Surface Properties |
| title | Surface-patterned electrode bioreactor for electrical stimulation |
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