Studies on isozymic variation among the South Indian species of Sphaerostephanos
Objective:To explore the identity and phylogenetic relationships among the three medicinally important species of Sphaerostephanos from South India using isozymic profile.Methods:The young fronds were homogenized with 3.5 mL of ice-cold homogenizing buffer in a pre-chilled pestle and mortar.The supe...
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| Veröffentlicht in: | Asian Pacific journal of tropical biomedicine 2011-08, Vol.1 (4), p.295-297 |
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| description | Objective:To explore the identity and phylogenetic relationships among the three medicinally important species of Sphaerostephanos from South India using isozymic profile.Methods:The young fronds were homogenized with 3.5 mL of ice-cold homogenizing buffer in a pre-chilled pestle and mortar.The supernatant was subjected to electrophoresis as described by Anbalagan poly acrylamide gel electrophoresis.Staining solutions for isoperoxidase was prepared as per Smila method for the detection of isoenzymes.Results:A total of six different bands in five different positions with different molecular weight/Rf values and four active zones have been observed in the isoperoxidase enzyme system of Sphaerostephanos.Only one band with MW/Rf 0.399 is common to two different species i.e.Sphaerostephanos arbuscula(S.arbuscula) and Sphaerostephanos unitus(S.unitus).Among the remaining four bands,two bands(R_f.0.23,0.47)are present in Sphaerostephanos subtruncatus(S.subtruncatus) and one distinct band has been observed individually in S.arbuscula(R_f.0.507) and S.unitus(R_f.0.56).Conclusions:The present preliminary molecular study through isozymic analysis shows the identity of all the three species and the present results confirm distinctness of these three species based on macromicromorphology,phytochemistry and cytology. |
| doi_str_mv | 10.1016/S2221-1691(11)60046-4 |
| format | Article |
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All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4479-21d09f4813037330e615496bcd0313de842876cb231a57e34631b4a689b7f8af3</citedby><cites>FETCH-LOGICAL-c4479-21d09f4813037330e615496bcd0313de842876cb231a57e34631b4a689b7f8af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71871X/71871X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614243/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614243/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23569778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Varaprasadham, Irudayaraj</creatorcontrib><creatorcontrib>Marimuthu, Johnson</creatorcontrib><title>Studies on isozymic variation among the South Indian species of Sphaerostephanos</title><title>Asian Pacific journal of tropical biomedicine</title><addtitle>Asian Pacific Journal of Tropical Biomedicine</addtitle><description>Objective:To explore the identity and phylogenetic relationships among the three medicinally important species of Sphaerostephanos from South India using isozymic profile.Methods:The young fronds were homogenized with 3.5 mL of ice-cold homogenizing buffer in a pre-chilled pestle and mortar.The supernatant was subjected to electrophoresis as described by Anbalagan poly acrylamide gel electrophoresis.Staining solutions for isoperoxidase was prepared as per Smila method for the detection of isoenzymes.Results:A total of six different bands in five different positions with different molecular weight/Rf values and four active zones have been observed in the isoperoxidase enzyme system of Sphaerostephanos.Only one band with MW/Rf 0.399 is common to two different species i.e.Sphaerostephanos arbuscula(S.arbuscula) and Sphaerostephanos unitus(S.unitus).Among the remaining four bands,two bands(R_f.0.23,0.47)are present in Sphaerostephanos subtruncatus(S.subtruncatus) and one distinct band has been observed individually in S.arbuscula(R_f.0.507) and S.unitus(R_f.0.56).Conclusions:The present preliminary molecular study through isozymic analysis shows the identity of all the three species and the present results confirm distinctness of these three species based on macromicromorphology,phytochemistry and cytology.</description><subject>acrylamide</subject><subject>electrophoresis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>gel</subject><subject>India</subject><subject>Inter-specific</subject><subject>Inter-specific variation</subject><subject>Isoenzymes - analysis</subject><subject>Isoperoxidase</subject><subject>Isozymic</subject><subject>Isozymic profile</subject><subject>Isozymic variation</subject><subject>Original</subject><subject>Peroxidases - analysis</subject><subject>Phylogenetic</subject><subject>Phylogenetic relationship</subject><subject>Plants, Medicinal - classification</subject><subject>Plants, Medicinal - enzymology</subject><subject>Poly</subject><subject>Poly acrylamide gel electrophoresis</subject><subject>profile</subject><subject>relationship</subject><subject>Sphaerostephanos</subject><subject>Staining and Labeling</subject><subject>Tracheophyta - classification</subject><subject>Tracheophyta - enzymology</subject><subject>variation</subject><issn>2221-1691</issn><issn>2588-9222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQtRCIVqU_ARSJSzkEPP6KcwFVFR-VKoG0cLYcZ7Ix2thbO1mp_Hrc3W1VTvjikefNm-f3CHkN9D1QUB9WjDGoQbVwAfBOUSpULZ6RUya1rtvSfF7qB8gJOc_5Ny1HUcakeElOGJeqbRp9Sn6s5qX3mKsYKp_jn7vJu2pnk7ezL092imFdzSNWq7jMY3Udem9Dlbfo9kNDtdqOFlPMM5YixPyKvBjsJuP58T4jv758_nn1rb75_vX66vKmdkI0bc2gp-0gNHDKG84pKpCiVZ3rKQfeoxZMN8p1jIOVDXKhOHTCKt12zaDtwM_IxwPvdukm7B2GOdmN2SY_2XRnovXm307wo1nHneEKBBO8EFwcCVK8XTDPZvLZ4WZjA8YlG-BMFmEgZYHKA9SVj-aEw-MaoOY-ELMPxNy7bQDMPhAjytybpxofpx7sL4BPBwAWp3Yek8nF1-Cw9wndbPro_7vi7VHaWJK69WH9RBst5moJLf8Lr7amDg</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Varaprasadham, Irudayaraj</creator><creator>Marimuthu, Johnson</creator><general>Elsevier B.V</general><general>Asian Pacific Tropical Medicine Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110801</creationdate><title>Studies on isozymic variation among the South Indian species of Sphaerostephanos</title><author>Varaprasadham, Irudayaraj ; Marimuthu, Johnson</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4479-21d09f4813037330e615496bcd0313de842876cb231a57e34631b4a689b7f8af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>acrylamide</topic><topic>electrophoresis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>gel</topic><topic>India</topic><topic>Inter-specific</topic><topic>Inter-specific variation</topic><topic>Isoenzymes - analysis</topic><topic>Isoperoxidase</topic><topic>Isozymic</topic><topic>Isozymic profile</topic><topic>Isozymic variation</topic><topic>Original</topic><topic>Peroxidases - analysis</topic><topic>Phylogenetic</topic><topic>Phylogenetic relationship</topic><topic>Plants, Medicinal - classification</topic><topic>Plants, Medicinal - enzymology</topic><topic>Poly</topic><topic>Poly acrylamide gel electrophoresis</topic><topic>profile</topic><topic>relationship</topic><topic>Sphaerostephanos</topic><topic>Staining and Labeling</topic><topic>Tracheophyta - classification</topic><topic>Tracheophyta - enzymology</topic><topic>variation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Varaprasadham, Irudayaraj</creatorcontrib><creatorcontrib>Marimuthu, Johnson</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Asian Pacific journal of tropical biomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Varaprasadham, Irudayaraj</au><au>Marimuthu, Johnson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on isozymic variation among the South Indian species of Sphaerostephanos</atitle><jtitle>Asian Pacific journal of tropical biomedicine</jtitle><addtitle>Asian Pacific Journal of Tropical Biomedicine</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>1</volume><issue>4</issue><spage>295</spage><epage>297</epage><pages>295-297</pages><issn>2221-1691</issn><eissn>2588-9222</eissn><abstract>Objective:To explore the identity and phylogenetic relationships among the three medicinally important species of Sphaerostephanos from South India using isozymic profile.Methods:The young fronds were homogenized with 3.5 mL of ice-cold homogenizing buffer in a pre-chilled pestle and mortar.The supernatant was subjected to electrophoresis as described by Anbalagan poly acrylamide gel electrophoresis.Staining solutions for isoperoxidase was prepared as per Smila method for the detection of isoenzymes.Results:A total of six different bands in five different positions with different molecular weight/Rf values and four active zones have been observed in the isoperoxidase enzyme system of Sphaerostephanos.Only one band with MW/Rf 0.399 is common to two different species i.e.Sphaerostephanos arbuscula(S.arbuscula) and Sphaerostephanos unitus(S.unitus).Among the remaining four bands,two bands(R_f.0.23,0.47)are present in Sphaerostephanos subtruncatus(S.subtruncatus) and one distinct band has been observed individually in S.arbuscula(R_f.0.507) and S.unitus(R_f.0.56).Conclusions:The present preliminary molecular study through isozymic analysis shows the identity of all the three species and the present results confirm distinctness of these three species based on macromicromorphology,phytochemistry and cytology.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>23569778</pmid><doi>10.1016/S2221-1691(11)60046-4</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | acrylamide electrophoresis Electrophoresis, Polyacrylamide Gel gel India Inter-specific Inter-specific variation Isoenzymes - analysis Isoperoxidase Isozymic Isozymic profile Isozymic variation Original Peroxidases - analysis Phylogenetic Phylogenetic relationship Plants, Medicinal - classification Plants, Medicinal - enzymology Poly Poly acrylamide gel electrophoresis profile relationship Sphaerostephanos Staining and Labeling Tracheophyta - classification Tracheophyta - enzymology variation |
| title | Studies on isozymic variation among the South Indian species of Sphaerostephanos |
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