Protection of cadmium chloride induced DNA damage by Lamiaceae plants
Objective:To analyze the total phenolic content,DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants,i.e.Anisomelos malabarica（A.malabarica）,Leucas aspera（L.aspera）and Ocimum basilicum（0.basilicum）.Methods:The total polyphenols and flavonoids were analy...
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| Veröffentlicht in: | Asian Pacific journal of tropical biomedicine 2011-10, Vol.1 (5), p.391-394 |
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| creator | Thirugnanasampandan, Ramaraj Jayakumar, Rajarajeswaran |
| description | Objective:To analyze the total phenolic content,DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants,i.e.Anisomelos malabarica（A.malabarica）,Leucas aspera（L.aspera）and Ocimum basilicum（0.basilicum）.Methods:The total polyphenols and flavonoids were analyzed in the ethanolic leaf extracts of the lamiaceae plants.To determine the DNA protecting activity,various concentrations of the plant extracts were prepared and treated on cultured HepG2 human lung cancer cells.The pretreated cells were exposed to H2O2to induce DNA damage through oxidative stress.Comet assay was done and the tail length of individual comets was measured.Nitric oxide and superoxide anion scavenging activities of lamiaceae plants were analyzed.Results:Among the three plant extracts,the highest amount of total phenolic content was found in O.basilicum（189.33 mg/g）,whereas A.malabarica showed high levels of flavonoids（10.66 mg/g）.O.basilicum also showed high levels of DNA protecting（85%）and radical scavenging activity.Conclusions:The results of this study shows that bioactive phenols present in lamiaceae plants may prevent carcinogenesis through scavenging free radicals and inhibiting DNA damage. |
| doi_str_mv | 10.1016/S2221-1691(11)60086-5 |
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All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4791-84b20b8cab86bb4eba59287e6542dc6d44dce0c41cbeb41f3d95f3f6902bdb443</citedby><cites>FETCH-LOGICAL-c4791-84b20b8cab86bb4eba59287e6542dc6d44dce0c41cbeb41f3d95f3f6902bdb443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71871X/71871X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614198/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614198/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23569799$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thirugnanasampandan, Ramaraj</creatorcontrib><creatorcontrib>Jayakumar, Rajarajeswaran</creatorcontrib><title>Protection of cadmium chloride induced DNA damage by Lamiaceae plants</title><title>Asian Pacific journal of tropical biomedicine</title><addtitle>Asian Pacific Journal of Tropical Biomedicine</addtitle><description>Objective:To analyze the total phenolic content,DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants,i.e.Anisomelos malabarica（A.malabarica）,Leucas aspera（L.aspera）and Ocimum basilicum（0.basilicum）.Methods:The total polyphenols and flavonoids were analyzed in the ethanolic leaf extracts of the lamiaceae plants.To determine the DNA protecting activity,various concentrations of the plant extracts were prepared and treated on cultured HepG2 human lung cancer cells.The pretreated cells were exposed to H2O2to induce DNA damage through oxidative stress.Comet assay was done and the tail length of individual comets was measured.Nitric oxide and superoxide anion scavenging activities of lamiaceae plants were analyzed.Results:Among the three plant extracts,the highest amount of total phenolic content was found in O.basilicum（189.33 mg/g）,whereas A.malabarica showed high levels of flavonoids（10.66 mg/g）.O.basilicum also showed high levels of DNA protecting（85%）and radical scavenging activity.Conclusions:The results of this study shows that bioactive phenols present in lamiaceae plants may prevent carcinogenesis through scavenging free radicals and inhibiting DNA damage.</description><subject>activity</subject><subject>Antioxidant</subject><subject>Antioxidant activity</subject><subject>assay</subject><subject>Cadmium</subject><subject>Cadmium chloride</subject><subject>Cadmium Chloride - toxicity</subject><subject>chloride</subject><subject>Comet</subject><subject>Comet Assay</subject><subject>damage</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>Free Radical Scavengers - chemistry</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Lamiaceae</subject><subject>Lamiaceae - chemistry</subject><subject>Lamiaceae plants</subject><subject>Leucas</subject><subject>Ocimum basilicum</subject><subject>Original</subject><subject>Phenols</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>plants</subject><subject>Protective Agents - chemistry</subject><subject>Protective Agents - pharmacology</subject><issn>2221-1691</issn><issn>2588-9222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9rFTEUxYNYbKn9CErAhboYm2SSTLJRSq1aeFRBXYf8ufNeZGbymswU-u1N-15LXZlNAvd37z05B6FXlHyghMrTn4wx2lCp6TtK30tClGzEM3TEhFKNrsXn9f2AHKKTUv6QeiRhTPAX6JC1QupO6yN08SOnGfwc04RTj70NY1xG7DdDyjEAjlNYPAT8-eoMBzvaNWB3i1d2jNaDBbwd7DSXl-igt0OBk_19jH5_ufh1_q1Zff96eX62ajzvNG0Ud4w45a1T0jkOzgrNVAdScBa8DJwHD8Rz6h04Tvs2aNG3vdSEueA4b4_Rx93c7eJGqPA0ZzuYbY6jzbcm2Wj-rUxxY9bpxrSScqpVHfB2PyCn6wXKbMZYPAz1F5CWYjTpaKdFRyopdqTPqZQM_eMWSsxdCOY-BHPnsKHU3IdgRO17_VTiY9eD5RX4tAOgGnUTIZviI0zV5JhrECak-N8Vb_bSNmlaX8dp_UQbaUmrBKftX87OotU</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Thirugnanasampandan, Ramaraj</creator><creator>Jayakumar, Rajarajeswaran</creator><general>Elsevier B.V</general><general>Asian Pacific Tropical Medicine Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>5PM</scope></search><sort><creationdate>20111001</creationdate><title>Protection of cadmium chloride induced DNA damage by Lamiaceae plants</title><author>Thirugnanasampandan, Ramaraj ; Jayakumar, Rajarajeswaran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4791-84b20b8cab86bb4eba59287e6542dc6d44dce0c41cbeb41f3d95f3f6902bdb443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>activity</topic><topic>Antioxidant</topic><topic>Antioxidant activity</topic><topic>assay</topic><topic>Cadmium</topic><topic>Cadmium chloride</topic><topic>Cadmium Chloride - toxicity</topic><topic>chloride</topic><topic>Comet</topic><topic>Comet Assay</topic><topic>damage</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>Free Radical Scavengers - chemistry</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Lamiaceae</topic><topic>Lamiaceae - chemistry</topic><topic>Lamiaceae plants</topic><topic>Leucas</topic><topic>Ocimum basilicum</topic><topic>Original</topic><topic>Phenols</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>plants</topic><topic>Protective Agents - chemistry</topic><topic>Protective Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thirugnanasampandan, Ramaraj</creatorcontrib><creatorcontrib>Jayakumar, Rajarajeswaran</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Asian Pacific journal of tropical biomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thirugnanasampandan, Ramaraj</au><au>Jayakumar, Rajarajeswaran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protection of cadmium chloride induced DNA damage by Lamiaceae plants</atitle><jtitle>Asian Pacific journal of tropical biomedicine</jtitle><addtitle>Asian Pacific Journal of Tropical Biomedicine</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>1</volume><issue>5</issue><spage>391</spage><epage>394</epage><pages>391-394</pages><issn>2221-1691</issn><eissn>2588-9222</eissn><abstract>Objective:To analyze the total phenolic content,DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants,i.e.Anisomelos malabarica（A.malabarica）,Leucas aspera（L.aspera）and Ocimum basilicum（0.basilicum）.Methods:The total polyphenols and flavonoids were analyzed in the ethanolic leaf extracts of the lamiaceae plants.To determine the DNA protecting activity,various concentrations of the plant extracts were prepared and treated on cultured HepG2 human lung cancer cells.The pretreated cells were exposed to H2O2to induce DNA damage through oxidative stress.Comet assay was done and the tail length of individual comets was measured.Nitric oxide and superoxide anion scavenging activities of lamiaceae plants were analyzed.Results:Among the three plant extracts,the highest amount of total phenolic content was found in O.basilicum（189.33 mg/g）,whereas A.malabarica showed high levels of flavonoids（10.66 mg/g）.O.basilicum also showed high levels of DNA protecting（85%）and radical scavenging activity.Conclusions:The results of this study shows that bioactive phenols present in lamiaceae plants may prevent carcinogenesis through scavenging free radicals and inhibiting DNA damage.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>23569799</pmid><doi>10.1016/S2221-1691(11)60086-5</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | activity Antioxidant Antioxidant activity assay Cadmium Cadmium chloride Cadmium Chloride - toxicity chloride Comet Comet Assay damage DNA DNA damage DNA Damage - drug effects Free Radical Scavengers - chemistry Free Radical Scavengers - pharmacology Hep G2 Cells Humans Lamiaceae Lamiaceae - chemistry Lamiaceae plants Leucas Ocimum basilicum Original Phenols Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry plants Protective Agents - chemistry Protective Agents - pharmacology |
| title | Protection of cadmium chloride induced DNA damage by Lamiaceae plants |
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