Interaction of avian influenza virus NS1 protein and nucleolar and coiled-body phosphoprotein 1
Nonstructural protein 1 (NS1) is a non-structural protein of avian influenza virus. It can interact with a variety of proteins of the host cells, enhancing the expression of viral proteins and changing the growth and metabolism of the host cells, thereby enhancing the virus’ pathogenicity and virule...
Gespeichert in:
| Veröffentlicht in: | Virus genes 2013-04, Vol.46 (2), p.287-292 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 292 |
|---|---|
| container_issue | 2 |
| container_start_page | 287 |
| container_title | Virus genes |
| container_volume | 46 |
| creator | Zhu, Chunyu Zheng, Fangliang Sun, Tingting Duan, Yanting Cao, Jingzhen Feng, Huawei Shang, Lingling Zhu, Ying Liu, Hongsheng |
| description | Nonstructural protein 1 (NS1) is a non-structural protein of avian influenza virus. It can interact with a variety of proteins of the host cells, enhancing the expression of viral proteins and changing the growth and metabolism of the host cells, thereby enhancing the virus’ pathogenicity and virulence. To investigate whether there are more host proteins that can interact with NS1 during viral infection, T7-phage display system was used to screen human lung cell cDNA library for proteins that could interact with NS1. One positive and specific clone was obtained and identified as nucleolar and coiled-body phosphoprotein 1(NOLC1). The interaction between these two proteins was further demonstrated by His-pull-down and co
-
immunoprecipitation experiments. Co-expression of both proteins in HeLa cell showed that NS1 and NOLC1 were co-localized in the cell’s nucleus. Gene truncation experiments revealed that the effector domain of NS1 was sufficient to interact with NOLC1. The results demonstrated a positive interaction between a viral NS1 and NOLC1 of the host cells, and provided a new target for drug screening. |
| doi_str_mv | 10.1007/s11262-012-0849-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3610028</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1348484019</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-e84f6ceac84819e39199faba761d1a40c2c9ef9d45269df1310d25c7cf477c563</originalsourceid><addsrcrecordid>eNqNkc9rFDEUx4NY7Lb6B3iRgBcvY_MyyUxyEaTYWih6UMFbyGZe2pTZZE1mFrp_fbNuW6ogeHiE8D7v-358CXkN7D0w1p8UAN7xhkENJXSzfUYWIHveaC1-PicLpjlrlOz0ITkq5YYxphQXL8ghb0Ep0HxBzEWcMFs3hRRp8tRugo00RD_OGLeWbkKeC_3yDeg6pwlDpDYONM5uxDTa_PvnUhhxaJZpuKXr61RqPMDwkhx4OxZ8df8ekx9nn76ffm4uv55fnH68bJxk7dSgEr5zaJ0SdS5sNWjt7dL2HQxgBXPcafR6EJJ3evDQAhu4dL3zou-d7Npj8mGvu56XKxwcxinb0axzWNl8a5IN5s9MDNfmKm1M29VTclUF3t0L5PRrxjKZVSgOx9FGTHMx0Io6m2Cg_wPl0Gupmajo27_QmzTnWC-xo7hsQcKuN-wpl1MpGf3j3MDMzmmzd9pUp83OabOtNW-eLvxY8WBtBfgeKDUVrzA_af1P1Tv1ubVE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1322531518</pqid></control><display><type>article</type><title>Interaction of avian influenza virus NS1 protein and nucleolar and coiled-body phosphoprotein 1</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Zhu, Chunyu ; Zheng, Fangliang ; Sun, Tingting ; Duan, Yanting ; Cao, Jingzhen ; Feng, Huawei ; Shang, Lingling ; Zhu, Ying ; Liu, Hongsheng</creator><creatorcontrib>Zhu, Chunyu ; Zheng, Fangliang ; Sun, Tingting ; Duan, Yanting ; Cao, Jingzhen ; Feng, Huawei ; Shang, Lingling ; Zhu, Ying ; Liu, Hongsheng</creatorcontrib><description>Nonstructural protein 1 (NS1) is a non-structural protein of avian influenza virus. It can interact with a variety of proteins of the host cells, enhancing the expression of viral proteins and changing the growth and metabolism of the host cells, thereby enhancing the virus’ pathogenicity and virulence. To investigate whether there are more host proteins that can interact with NS1 during viral infection, T7-phage display system was used to screen human lung cell cDNA library for proteins that could interact with NS1. One positive and specific clone was obtained and identified as nucleolar and coiled-body phosphoprotein 1(NOLC1). The interaction between these two proteins was further demonstrated by His-pull-down and co
-
immunoprecipitation experiments. Co-expression of both proteins in HeLa cell showed that NS1 and NOLC1 were co-localized in the cell’s nucleus. Gene truncation experiments revealed that the effector domain of NS1 was sufficient to interact with NOLC1. The results demonstrated a positive interaction between a viral NS1 and NOLC1 of the host cells, and provided a new target for drug screening.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-012-0849-z</identifier><identifier>PMID: 23188192</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Avian influenza virus ; Biomedical and Life Sciences ; Biomedicine ; HeLa Cells ; Humans ; Influenza A Virus, H5N1 Subtype - genetics ; Influenza A Virus, H5N1 Subtype - metabolism ; Influenza, Human - genetics ; Influenza, Human - metabolism ; Influenza, Human - virology ; Medical Microbiology ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; Plant Sciences ; Protein Binding ; Viral Nonstructural Proteins - genetics ; Viral Nonstructural Proteins - metabolism ; Virology</subject><ispartof>Virus genes, 2013-04, Vol.46 (2), p.287-292</ispartof><rights>The Author(s) 2012</rights><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-e84f6ceac84819e39199faba761d1a40c2c9ef9d45269df1310d25c7cf477c563</citedby><cites>FETCH-LOGICAL-c503t-e84f6ceac84819e39199faba761d1a40c2c9ef9d45269df1310d25c7cf477c563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-012-0849-z$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-012-0849-z$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23188192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Chunyu</creatorcontrib><creatorcontrib>Zheng, Fangliang</creatorcontrib><creatorcontrib>Sun, Tingting</creatorcontrib><creatorcontrib>Duan, Yanting</creatorcontrib><creatorcontrib>Cao, Jingzhen</creatorcontrib><creatorcontrib>Feng, Huawei</creatorcontrib><creatorcontrib>Shang, Lingling</creatorcontrib><creatorcontrib>Zhu, Ying</creatorcontrib><creatorcontrib>Liu, Hongsheng</creatorcontrib><title>Interaction of avian influenza virus NS1 protein and nucleolar and coiled-body phosphoprotein 1</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>Nonstructural protein 1 (NS1) is a non-structural protein of avian influenza virus. It can interact with a variety of proteins of the host cells, enhancing the expression of viral proteins and changing the growth and metabolism of the host cells, thereby enhancing the virus’ pathogenicity and virulence. To investigate whether there are more host proteins that can interact with NS1 during viral infection, T7-phage display system was used to screen human lung cell cDNA library for proteins that could interact with NS1. One positive and specific clone was obtained and identified as nucleolar and coiled-body phosphoprotein 1(NOLC1). The interaction between these two proteins was further demonstrated by His-pull-down and co
-
immunoprecipitation experiments. Co-expression of both proteins in HeLa cell showed that NS1 and NOLC1 were co-localized in the cell’s nucleus. Gene truncation experiments revealed that the effector domain of NS1 was sufficient to interact with NOLC1. The results demonstrated a positive interaction between a viral NS1 and NOLC1 of the host cells, and provided a new target for drug screening.</description><subject>Avian influenza virus</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Influenza A Virus, H5N1 Subtype - genetics</subject><subject>Influenza A Virus, H5N1 Subtype - metabolism</subject><subject>Influenza, Human - genetics</subject><subject>Influenza, Human - metabolism</subject><subject>Influenza, Human - virology</subject><subject>Medical Microbiology</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>Plant Sciences</subject><subject>Protein Binding</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>Viral Nonstructural Proteins - metabolism</subject><subject>Virology</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc9rFDEUx4NY7Lb6B3iRgBcvY_MyyUxyEaTYWih6UMFbyGZe2pTZZE1mFrp_fbNuW6ogeHiE8D7v-358CXkN7D0w1p8UAN7xhkENJXSzfUYWIHveaC1-PicLpjlrlOz0ITkq5YYxphQXL8ghb0Ep0HxBzEWcMFs3hRRp8tRugo00RD_OGLeWbkKeC_3yDeg6pwlDpDYONM5uxDTa_PvnUhhxaJZpuKXr61RqPMDwkhx4OxZ8df8ekx9nn76ffm4uv55fnH68bJxk7dSgEr5zaJ0SdS5sNWjt7dL2HQxgBXPcafR6EJJ3evDQAhu4dL3zou-d7Npj8mGvu56XKxwcxinb0axzWNl8a5IN5s9MDNfmKm1M29VTclUF3t0L5PRrxjKZVSgOx9FGTHMx0Io6m2Cg_wPl0Gupmajo27_QmzTnWC-xo7hsQcKuN-wpl1MpGf3j3MDMzmmzd9pUp83OabOtNW-eLvxY8WBtBfgeKDUVrzA_af1P1Tv1ubVE</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Zhu, Chunyu</creator><creator>Zheng, Fangliang</creator><creator>Sun, Tingting</creator><creator>Duan, Yanting</creator><creator>Cao, Jingzhen</creator><creator>Feng, Huawei</creator><creator>Shang, Lingling</creator><creator>Zhu, Ying</creator><creator>Liu, Hongsheng</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130401</creationdate><title>Interaction of avian influenza virus NS1 protein and nucleolar and coiled-body phosphoprotein 1</title><author>Zhu, Chunyu ; Zheng, Fangliang ; Sun, Tingting ; Duan, Yanting ; Cao, Jingzhen ; Feng, Huawei ; Shang, Lingling ; Zhu, Ying ; Liu, Hongsheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-e84f6ceac84819e39199faba761d1a40c2c9ef9d45269df1310d25c7cf477c563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Avian influenza virus</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Influenza A Virus, H5N1 Subtype - genetics</topic><topic>Influenza A Virus, H5N1 Subtype - metabolism</topic><topic>Influenza, Human - genetics</topic><topic>Influenza, Human - metabolism</topic><topic>Influenza, Human - virology</topic><topic>Medical Microbiology</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>Plant Sciences</topic><topic>Protein Binding</topic><topic>Viral Nonstructural Proteins - genetics</topic><topic>Viral Nonstructural Proteins - metabolism</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Chunyu</creatorcontrib><creatorcontrib>Zheng, Fangliang</creatorcontrib><creatorcontrib>Sun, Tingting</creatorcontrib><creatorcontrib>Duan, Yanting</creatorcontrib><creatorcontrib>Cao, Jingzhen</creatorcontrib><creatorcontrib>Feng, Huawei</creatorcontrib><creatorcontrib>Shang, Lingling</creatorcontrib><creatorcontrib>Zhu, Ying</creatorcontrib><creatorcontrib>Liu, Hongsheng</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Chunyu</au><au>Zheng, Fangliang</au><au>Sun, Tingting</au><au>Duan, Yanting</au><au>Cao, Jingzhen</au><au>Feng, Huawei</au><au>Shang, Lingling</au><au>Zhu, Ying</au><au>Liu, Hongsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of avian influenza virus NS1 protein and nucleolar and coiled-body phosphoprotein 1</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>46</volume><issue>2</issue><spage>287</spage><epage>292</epage><pages>287-292</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Nonstructural protein 1 (NS1) is a non-structural protein of avian influenza virus. It can interact with a variety of proteins of the host cells, enhancing the expression of viral proteins and changing the growth and metabolism of the host cells, thereby enhancing the virus’ pathogenicity and virulence. To investigate whether there are more host proteins that can interact with NS1 during viral infection, T7-phage display system was used to screen human lung cell cDNA library for proteins that could interact with NS1. One positive and specific clone was obtained and identified as nucleolar and coiled-body phosphoprotein 1(NOLC1). The interaction between these two proteins was further demonstrated by His-pull-down and co
-
immunoprecipitation experiments. Co-expression of both proteins in HeLa cell showed that NS1 and NOLC1 were co-localized in the cell’s nucleus. Gene truncation experiments revealed that the effector domain of NS1 was sufficient to interact with NOLC1. The results demonstrated a positive interaction between a viral NS1 and NOLC1 of the host cells, and provided a new target for drug screening.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23188192</pmid><doi>10.1007/s11262-012-0849-z</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-8569 |
| ispartof | Virus genes, 2013-04, Vol.46 (2), p.287-292 |
| issn | 0920-8569 1572-994X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3610028 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Avian influenza virus Biomedical and Life Sciences Biomedicine HeLa Cells Humans Influenza A Virus, H5N1 Subtype - genetics Influenza A Virus, H5N1 Subtype - metabolism Influenza, Human - genetics Influenza, Human - metabolism Influenza, Human - virology Medical Microbiology Nuclear Proteins - genetics Nuclear Proteins - metabolism Phosphoproteins - genetics Phosphoproteins - metabolism Plant Sciences Protein Binding Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - metabolism Virology |
| title | Interaction of avian influenza virus NS1 protein and nucleolar and coiled-body phosphoprotein 1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T20%3A19%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Interaction%20of%20avian%20influenza%20virus%20NS1%20protein%20and%20nucleolar%20and%20coiled-body%20phosphoprotein%201&rft.jtitle=Virus%20genes&rft.au=Zhu,%20Chunyu&rft.date=2013-04-01&rft.volume=46&rft.issue=2&rft.spage=287&rft.epage=292&rft.pages=287-292&rft.issn=0920-8569&rft.eissn=1572-994X&rft_id=info:doi/10.1007/s11262-012-0849-z&rft_dat=%3Cproquest_pubme%3E1348484019%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1322531518&rft_id=info:pmid/23188192&rfr_iscdi=true |