Molecular study of HCV detection,genotypes and their routes of transmission in North West Frontier Province,Pakistan
Objective:To determine hepatitis C virus(HCV) genotypes and explore the associated risk factors in chronic HCY patients.Methods:A total of 116 patients with chronic hepatitis C were subjected to polymerase chain reaction(PCR) based detection out of which 112 chronic HCV patients[53 male(47.32%),59 f...
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| Veröffentlicht in: | Asian Pacific journal of tropical biomedicine 2012-07, Vol.2 (7), p.532-536 |
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| creator | Safi, Asher Zaman Waheed, Yasir Sadat, Joharia Solat-Ul-Islam Salahuddin, Sadia Saeed, Umar Ashraf, Muhammad |
| description | Objective:To determine hepatitis C virus(HCV) genotypes and explore the associated risk factors in chronic HCY patients.Methods:A total of 116 patients with chronic hepatitis C were subjected to polymerase chain reaction(PCR) based detection out of which 112 chronic HCV patients[53 male(47.32%),59 female(52.68%);mean age(43.76±16.40) years;mean BMI(23.8± 3.9)kg]were enrolled in this study.The frequency of 6 HCV genotypes and associated risk factors were evaluated from five districts of North West Frontier Province(NWFP).Results:Genotype 3was the most prevalent in 73 samples(65.17%) followed by genotype 1 in 24(21.42%) and genotype2 in 13(11.60%) samples.Genotype3 had significantly high prevalence(P=0.0002).The results showed that 48(42.85%) samples were infected with HCV 3a:25(22.32%) with 3b;14(12.50%) with 1a;10(8.92%)with 1b;11(9.82%) with 2a;2(1.78%) with 2b;and 2 were untypable.The distribution of HCV genotypes in Murdan.Charsadda,Peshawar.Sawabi and Nowshehra districts was different.Use of unsterile equipment lor medication,barbers and previous history of hospitalization were the main risk factors for HCV transmission.Conclusions:Cenotype 3a and 3b.1a.1b and 2a are the common genotypes in NWFP.Genotype 4.5.and 6 can not be found in a single sample.The level of awareness about various modes ot transmission of HCV among the population is found to be verv low. |
| doi_str_mv | 10.1016/S2221-1691(12)60091-4 |
| format | Article |
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All rights reserved. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4476-9d8a0f8dc9f5bd830422772b3f2335644cbb03e950e68a547390667d9e1358653</citedby><cites>FETCH-LOGICAL-c4476-9d8a0f8dc9f5bd830422772b3f2335644cbb03e950e68a547390667d9e1358653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71871X/71871X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609335/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609335/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23569965$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Safi, Asher Zaman</creatorcontrib><creatorcontrib>Waheed, Yasir</creatorcontrib><creatorcontrib>Sadat, Joharia</creatorcontrib><creatorcontrib>Solat-Ul-Islam</creatorcontrib><creatorcontrib>Salahuddin, Sadia</creatorcontrib><creatorcontrib>Saeed, Umar</creatorcontrib><creatorcontrib>Ashraf, Muhammad</creatorcontrib><title>Molecular study of HCV detection,genotypes and their routes of transmission in North West Frontier Province,Pakistan</title><title>Asian Pacific journal of tropical biomedicine</title><addtitle>Asian Pacific Journal of Tropical Biomedicine</addtitle><description>Objective:To determine hepatitis C virus(HCV) genotypes and explore the associated risk factors in chronic HCY patients.Methods:A total of 116 patients with chronic hepatitis C were subjected to polymerase chain reaction(PCR) based detection out of which 112 chronic HCV patients[53 male(47.32%),59 female(52.68%);mean age(43.76±16.40) years;mean BMI(23.8± 3.9)kg]were enrolled in this study.The frequency of 6 HCV genotypes and associated risk factors were evaluated from five districts of North West Frontier Province(NWFP).Results:Genotype 3was the most prevalent in 73 samples(65.17%) followed by genotype 1 in 24(21.42%) and genotype2 in 13(11.60%) samples.Genotype3 had significantly high prevalence(P=0.0002).The results showed that 48(42.85%) samples were infected with HCV 3a:25(22.32%) with 3b;14(12.50%) with 1a;10(8.92%)with 1b;11(9.82%) with 2a;2(1.78%) with 2b;and 2 were untypable.The distribution of HCV genotypes in Murdan.Charsadda,Peshawar.Sawabi and Nowshehra districts was different.Use of unsterile equipment lor medication,barbers and previous history of hospitalization were the main risk factors for HCV transmission.Conclusions:Cenotype 3a and 3b.1a.1b and 2a are the common genotypes in NWFP.Genotype 4.5.and 6 can not be found in a single sample.The level of awareness about various modes ot transmission of HCV among the population is found to be verv low.</description><subject>Adult</subject><subject>Disease Transmission, Infectious</subject><subject>factors</subject><subject>Female</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Genotyping Techniques</subject><subject>HCV</subject><subject>Hepacivirus - classification</subject><subject>Hepacivirus - genetics</subject><subject>Hepacivirus - isolation & purification</subject><subject>Hepatitis C, Chronic - epidemiology</subject><subject>Hepatitis C, Chronic - transmission</subject><subject>Hepatitis C, Chronic - virology</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular</subject><subject>Molecular Epidemiology</subject><subject>Molecular study</subject><subject>NWFP</subject><subject>Original</subject><subject>Pakistan - epidemiology</subject><subject>PCR</subject><subject>Polymerase Chain Reaction</subject><subject>Risk</subject><subject>Risk Factors</subject><subject>RNA, Viral - genetics</subject><subject>study</subject><subject>Subtype</subject><subject>Subtype 3a</subject><subject>Transmission</subject><issn>2221-1691</issn><issn>2588-9222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1vEzEUtBCIVqE_AWSJS5G64I-1d30BoYhSpAKV-Dpaju1NDBs7tb2R8u95bdKqnPDFlt-8eTNvEHpOyWtKqHzzjTFGGyoVPaXslSRE0aZ9hI6Z6PtGQfExvO8gR-iklN8EjiSMifYpOmJcSKWkOEb1cxq9nUaTcamT2-E04Iv5T-x89baGFM-WPqa62_iCTXS4rnzIOKepwgdgazaxrEMpAMUh4i8p1xX-5UvF5znFGnzGVzltQ7T-7Mr8CaWa-Aw9GcxY_MnhnqEf5x--zy-ay68fP83fXza2bTvZKNcbMvTOqkEsXM9Jy1jXsQUfGAcDbWsXC8K9EsTL3oi244pI2TnlKRe9FHyG3u55N9Ni7Z31EeSOepPD2uSdTibofysxrPQybTWXRMEIIDg9EOR0PYEpDVatH0cTfZqKppwJzgkBPTMk9lCbUynZD_djKNE3oenb0PRNIpoyfRuabqHvxUON9113EQHg3R7gYVNb2KcuNnhYpwsZItIuhf-OeHmQtkpxeR3i8oE2wgnvwSr_C0TOs2o</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Safi, Asher Zaman</creator><creator>Waheed, Yasir</creator><creator>Sadat, Joharia</creator><creator>Solat-Ul-Islam</creator><creator>Salahuddin, Sadia</creator><creator>Saeed, Umar</creator><creator>Ashraf, Muhammad</creator><general>Elsevier B.V</general><general>Asian Pacific Tropical Medicine Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120701</creationdate><title>Molecular study of HCV detection,genotypes and their routes of transmission in North West Frontier Province,Pakistan</title><author>Safi, Asher Zaman ; Waheed, Yasir ; Sadat, Joharia ; Solat-Ul-Islam ; Salahuddin, Sadia ; Saeed, Umar ; Ashraf, Muhammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4476-9d8a0f8dc9f5bd830422772b3f2335644cbb03e950e68a547390667d9e1358653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Disease Transmission, Infectious</topic><topic>factors</topic><topic>Female</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Genotyping Techniques</topic><topic>HCV</topic><topic>Hepacivirus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Asian Pacific journal of tropical biomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Safi, Asher Zaman</au><au>Waheed, Yasir</au><au>Sadat, Joharia</au><au>Solat-Ul-Islam</au><au>Salahuddin, Sadia</au><au>Saeed, Umar</au><au>Ashraf, Muhammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular study of HCV detection,genotypes and their routes of transmission in North West Frontier Province,Pakistan</atitle><jtitle>Asian Pacific journal of tropical biomedicine</jtitle><addtitle>Asian Pacific Journal of Tropical Biomedicine</addtitle><date>2012-07-01</date><risdate>2012</risdate><volume>2</volume><issue>7</issue><spage>532</spage><epage>536</epage><pages>532-536</pages><issn>2221-1691</issn><eissn>2588-9222</eissn><abstract>Objective:To determine hepatitis C virus(HCV) genotypes and explore the associated risk factors in chronic HCY patients.Methods:A total of 116 patients with chronic hepatitis C were subjected to polymerase chain reaction(PCR) based detection out of which 112 chronic HCV patients[53 male(47.32%),59 female(52.68%);mean age(43.76±16.40) years;mean BMI(23.8± 3.9)kg]were enrolled in this study.The frequency of 6 HCV genotypes and associated risk factors were evaluated from five districts of North West Frontier Province(NWFP).Results:Genotype 3was the most prevalent in 73 samples(65.17%) followed by genotype 1 in 24(21.42%) and genotype2 in 13(11.60%) samples.Genotype3 had significantly high prevalence(P=0.0002).The results showed that 48(42.85%) samples were infected with HCV 3a:25(22.32%) with 3b;14(12.50%) with 1a;10(8.92%)with 1b;11(9.82%) with 2a;2(1.78%) with 2b;and 2 were untypable.The distribution of HCV genotypes in Murdan.Charsadda,Peshawar.Sawabi and Nowshehra districts was different.Use of unsterile equipment lor medication,barbers and previous history of hospitalization were the main risk factors for HCV transmission.Conclusions:Cenotype 3a and 3b.1a.1b and 2a are the common genotypes in NWFP.Genotype 4.5.and 6 can not be found in a single sample.The level of awareness about various modes ot transmission of HCV among the population is found to be verv low.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>23569965</pmid><doi>10.1016/S2221-1691(12)60091-4</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Disease Transmission, Infectious factors Female Genotype Genotypes Genotyping Techniques HCV Hepacivirus - classification Hepacivirus - genetics Hepacivirus - isolation & purification Hepatitis C, Chronic - epidemiology Hepatitis C, Chronic - transmission Hepatitis C, Chronic - virology Humans Male Middle Aged Molecular Molecular Epidemiology Molecular study NWFP Original Pakistan - epidemiology PCR Polymerase Chain Reaction Risk Risk Factors RNA, Viral - genetics study Subtype Subtype 3a Transmission |
| title | Molecular study of HCV detection,genotypes and their routes of transmission in North West Frontier Province,Pakistan |
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