Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study
Red blood cells (RBCs) are highly deformable and possess a robust membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultrastructure, where these cells are elongated and twist around spontaneously formed fibrin fibers. These changes may...
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| Veröffentlicht in: | Cardiovascular Diabetology 2013-01, Vol.12 (1), p.25-25, Article 25 |
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| creator | Buys, Antoinette V Van Rooy, Mia-Jean Soma, Prashilla Van Papendorp, Dirk Lipinski, Boguslaw Pretorius, Etheresia |
| description | Red blood cells (RBCs) are highly deformable and possess a robust membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultrastructure, where these cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, cell shape and roughness of RBCs may be tracked, as this cell's health status is crucial to the overall wellness of the diabetic patient. |
| doi_str_mv | 10.1186/1475-2840-12-25 |
| format | Article |
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Previous research showed that in diabetic patients, there is a changed RBC ultrastructure, where these cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, cell shape and roughness of RBCs may be tracked, as this cell's health status is crucial to the overall wellness of the diabetic patient.</description><identifier>ISSN: 1475-2840</identifier><identifier>EISSN: 1475-2840</identifier><identifier>DOI: 10.1186/1475-2840-12-25</identifier><identifier>PMID: 23356738</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Atomic force microscopy ; Blood ; Care and treatment ; Cell Membrane Structures - pathology ; Cell Membrane Structures - ultrastructure ; Databases, Factual ; Diabetes ; Diabetes Mellitus, Type 2 - pathology ; Erythrocytes ; Erythrocytes - pathology ; Erythrocytes - ultrastructure ; Female ; Fibrin ; Humans ; Male ; Medical research ; Medicine, Experimental ; Microscopy, Atomic Force - methods ; Microscopy, Electron, Scanning - methods ; Original Investigation ; Patient outcomes ; Physiological aspects ; Proteins ; Risk factors ; Studies ; Surface Properties ; Type 2 diabetes</subject><ispartof>Cardiovascular Diabetology, 2013-01, Vol.12 (1), p.25-25, Article 25</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Buys et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright ©2013 Buys et al; licensee BioMed Central Ltd. 2013 Buys et al; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b547t-d18be123f357112b79680060a3efab128fd1f6286310d98b2bea959db325ab713</citedby><cites>FETCH-LOGICAL-b547t-d18be123f357112b79680060a3efab128fd1f6286310d98b2bea959db325ab713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599682/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599682/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23356738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buys, Antoinette V</creatorcontrib><creatorcontrib>Van Rooy, Mia-Jean</creatorcontrib><creatorcontrib>Soma, Prashilla</creatorcontrib><creatorcontrib>Van Papendorp, Dirk</creatorcontrib><creatorcontrib>Lipinski, Boguslaw</creatorcontrib><creatorcontrib>Pretorius, Etheresia</creatorcontrib><title>Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study</title><title>Cardiovascular Diabetology</title><addtitle>Cardiovasc Diabetol</addtitle><description>Red blood cells (RBCs) are highly deformable and possess a robust membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultrastructure, where these cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, cell shape and roughness of RBCs may be tracked, as this cell's health status is crucial to the overall wellness of the diabetic patient.</description><subject>Atomic force microscopy</subject><subject>Blood</subject><subject>Care and treatment</subject><subject>Cell Membrane Structures - pathology</subject><subject>Cell Membrane Structures - ultrastructure</subject><subject>Databases, Factual</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Type 2 - pathology</subject><subject>Erythrocytes</subject><subject>Erythrocytes - pathology</subject><subject>Erythrocytes - ultrastructure</subject><subject>Female</subject><subject>Fibrin</subject><subject>Humans</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Microscopy, Atomic Force - methods</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Original Investigation</subject><subject>Patient outcomes</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Risk factors</subject><subject>Studies</subject><subject>Surface Properties</subject><subject>Type 2 diabetes</subject><issn>1475-2840</issn><issn>1475-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kk1v1DAQhiMEoqVw5oYsceGS1mPHicMBqaygRarEBc6WPyZbV4m92Eml_fc42rJqEcgHjzyvX4-fmap6C_QcQLYX0HSiZrKhNbCaiWfV6fHk-aP4pHqV8x2l0MkWXlYnjHPRdlyeVvebWx22mIkPJKEjZozREYvjSCacTNIBSZ7TYucl4Sqa9zskjDivDc6YPxJNstUh-LAlOKKdUwxEB0f0HCdvyRCTRVKiFLONu31xW9z-dfVi0GPGNw_7WfXz65cfm-v65vvVt83lTW1E0821A2kQGB-46ACY6fpWUtpSzXHQBpgcHAwtky0H6nppmEHdi94ZzoQ2HfCz6tPBd7eYCZ3FMCc9ql3yk057FbVXTzPB36ptvFdc9OUtVgw-HwyMj_8xeJqxcVIrd7VyV8AUE8Xkw0MVKf5aMM9q8nllXOjGJSvgpTGNFB0t0vd_Se_ikkJhtKpaTvum6Yvq_KDa6hGVD0Msb9uyHBbSMeDgy_ml4E0rgPcrh4vDhbUNOeFw_AGUIssk_aPmd4_JHfV_Rof_BjzexME</recordid><startdate>20130128</startdate><enddate>20130128</enddate><creator>Buys, Antoinette V</creator><creator>Van Rooy, Mia-Jean</creator><creator>Soma, Prashilla</creator><creator>Van Papendorp, Dirk</creator><creator>Lipinski, Boguslaw</creator><creator>Pretorius, Etheresia</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130128</creationdate><title>Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study</title><author>Buys, Antoinette V ; Van Rooy, Mia-Jean ; Soma, Prashilla ; Van Papendorp, Dirk ; Lipinski, Boguslaw ; Pretorius, Etheresia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b547t-d18be123f357112b79680060a3efab128fd1f6286310d98b2bea959db325ab713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Atomic force microscopy</topic><topic>Blood</topic><topic>Care and treatment</topic><topic>Cell Membrane Structures - pathology</topic><topic>Cell Membrane Structures - ultrastructure</topic><topic>Databases, Factual</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Type 2 - pathology</topic><topic>Erythrocytes</topic><topic>Erythrocytes - pathology</topic><topic>Erythrocytes - ultrastructure</topic><topic>Female</topic><topic>Fibrin</topic><topic>Humans</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Microscopy, Atomic Force - methods</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Original Investigation</topic><topic>Patient outcomes</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Risk factors</topic><topic>Studies</topic><topic>Surface Properties</topic><topic>Type 2 diabetes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buys, Antoinette V</creatorcontrib><creatorcontrib>Van Rooy, Mia-Jean</creatorcontrib><creatorcontrib>Soma, Prashilla</creatorcontrib><creatorcontrib>Van Papendorp, Dirk</creatorcontrib><creatorcontrib>Lipinski, Boguslaw</creatorcontrib><creatorcontrib>Pretorius, Etheresia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cardiovascular Diabetology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buys, Antoinette V</au><au>Van Rooy, Mia-Jean</au><au>Soma, Prashilla</au><au>Van Papendorp, Dirk</au><au>Lipinski, Boguslaw</au><au>Pretorius, Etheresia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study</atitle><jtitle>Cardiovascular Diabetology</jtitle><addtitle>Cardiovasc Diabetol</addtitle><date>2013-01-28</date><risdate>2013</risdate><volume>12</volume><issue>1</issue><spage>25</spage><epage>25</epage><pages>25-25</pages><artnum>25</artnum><issn>1475-2840</issn><eissn>1475-2840</eissn><abstract>Red blood cells (RBCs) are highly deformable and possess a robust membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultrastructure, where these cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, cell shape and roughness of RBCs may be tracked, as this cell's health status is crucial to the overall wellness of the diabetic patient.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>23356738</pmid><doi>10.1186/1475-2840-12-25</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Atomic force microscopy Blood Care and treatment Cell Membrane Structures - pathology Cell Membrane Structures - ultrastructure Databases, Factual Diabetes Diabetes Mellitus, Type 2 - pathology Erythrocytes Erythrocytes - pathology Erythrocytes - ultrastructure Female Fibrin Humans Male Medical research Medicine, Experimental Microscopy, Atomic Force - methods Microscopy, Electron, Scanning - methods Original Investigation Patient outcomes Physiological aspects Proteins Risk factors Studies Surface Properties Type 2 diabetes |
| title | Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study |
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