Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving
Rationale and objectives Responding to heroin cues progressively increases after cessation of heroin self-administration (incubation of heroin craving). We investigated whether this incubation is associated with time-dependent changes in brain-derived neurotrophic factor (BDNF) and methyl-CpG bindin...
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| Veröffentlicht in: | Psychopharmacologia 2012-12, Vol.224 (4), p.559-571 |
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| creator | Theberge, Florence R. M. Pickens, Charles L. Goldart, Evan Fanous, Sanya Hope, Bruce T. Liu, Qing-Rong Shaham, Yavin |
| description | Rationale and objectives
Responding to heroin cues progressively increases after cessation of heroin self-administration (incubation of heroin craving). We investigated whether this incubation is associated with time-dependent changes in brain-derived neurotrophic factor (BDNF) and methyl-CpG binding protein 2 (MeCP2) signaling and mu opioid receptor (MOR) expression in nucleus accumbens (NAc), dorsal striatum (DS), and medial prefrontal cortex (mPFC). We also investigated the effect of the preferential MOR antagonist naloxone on cue-induced heroin seeking during abstinence.
Methods
We trained rats to self-administer heroin or saline for 9–10 days and then dissected the NAc, DS, and mPFC at different abstinence days and measured mRNA and protein levels of BDNF, TrkB, and MeCP2, as well as MOR mRNA (
Oprm1
). In other groups, we assessed cue-induced heroin seeking in extinction tests after 1, 11, and 30 abstinence days, and naloxone’s (0–1.0 mg/kg) effect on extinction responding after 1 and 15 days.
Results
Cue-induced heroin seeking progressively increased or incubated during abstinence. This incubation was not associated with changes in BDNF, TrkB, or MeCP2 mRNA or protein levels in NAc, DS, or mPFC; additionally, no molecular changes were observed after extinction tests on day 11. In NAc, but not DS or mPFC, MOR mRNA decreased on abstinence day 1 and returned to basal levels over time. Naloxone significantly decreased cue-induced heroin seeking after 15 abstinence days but not 1 day.
Conclusions
Results suggest a role of MOR in incubation of heroin craving. As previous studies implicated NAc BDNF in incubation of cocaine craving, our data suggest that different mechanisms contribute to incubation of heroin versus cocaine craving. |
| doi_str_mv | 10.1007/s00213-012-2784-z |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3593041</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A354146327</galeid><sourcerecordid>A354146327</sourcerecordid><originalsourceid>FETCH-LOGICAL-c600t-e40acaff047fe33d4f3b1ff54bc243dcea72122a5ded2f990a3e389fbb2ebe513</originalsourceid><addsrcrecordid>eNp1kt9u0zAUxiMEYmXwANwgSwiJi2b4X-r0BqkrDJDGQGhcW45z3HokdrCdAXsz3g6Xlm5D4FxYJ-f3fScn-oriMcFHBGPxImJMCSsxoSUVNS-v7hQTwlmusKB3iwnGjJWMVPVB8SDGC5wPr_n94oBSMce14JPi5yJGr61K1jvkDUq2h7KFAVwLLiG9Vm4FEVmH-hH5wXrbogAahuQD6j-dLaaoGRNyPqHjV2cnU3QevhxPUW6-h-VH-htByrVoCD5BtoHvQ4AYN-NyldaAgsr6UXcwRqS0HvsGXETfbFpnQo_N_tvWEHzW6KAurVs9LO4Z1UV4tLsPi88nr8-Xb8vTD2_eLRenpZ5hnErgWGllDObCAGMtN6whxlS80ZSzVoMSlFCqqhZaauZzrBiwem6ahkIDFWGHxcut7zA2PWSBS0F1cgi2V-GH9MrK2x1n13LlLyWr5gzzjcHznUHwX0eISfY2aug65cCPURJaCSEI4TyjT_9CL_wYXF5PEjIXpCaMVdfUSnUgrTM-z9UbU7lgFSd8xqjI1NE_qPy00FvtHRib398SkK1ABx9jALPfkWC5yZvc5k3mvMlN3uRV1jy5-XP2ij8By8CzHaCiVp0Jymkbr7nZjOOqnmWObrmYWzly4cbm_53-Cy0J738</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1197181335</pqid></control><display><type>article</type><title>Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Theberge, Florence R. M. ; Pickens, Charles L. ; Goldart, Evan ; Fanous, Sanya ; Hope, Bruce T. ; Liu, Qing-Rong ; Shaham, Yavin</creator><creatorcontrib>Theberge, Florence R. M. ; Pickens, Charles L. ; Goldart, Evan ; Fanous, Sanya ; Hope, Bruce T. ; Liu, Qing-Rong ; Shaham, Yavin</creatorcontrib><description>Rationale and objectives
Responding to heroin cues progressively increases after cessation of heroin self-administration (incubation of heroin craving). We investigated whether this incubation is associated with time-dependent changes in brain-derived neurotrophic factor (BDNF) and methyl-CpG binding protein 2 (MeCP2) signaling and mu opioid receptor (MOR) expression in nucleus accumbens (NAc), dorsal striatum (DS), and medial prefrontal cortex (mPFC). We also investigated the effect of the preferential MOR antagonist naloxone on cue-induced heroin seeking during abstinence.
Methods
We trained rats to self-administer heroin or saline for 9–10 days and then dissected the NAc, DS, and mPFC at different abstinence days and measured mRNA and protein levels of BDNF, TrkB, and MeCP2, as well as MOR mRNA (
Oprm1
). In other groups, we assessed cue-induced heroin seeking in extinction tests after 1, 11, and 30 abstinence days, and naloxone’s (0–1.0 mg/kg) effect on extinction responding after 1 and 15 days.
Results
Cue-induced heroin seeking progressively increased or incubated during abstinence. This incubation was not associated with changes in BDNF, TrkB, or MeCP2 mRNA or protein levels in NAc, DS, or mPFC; additionally, no molecular changes were observed after extinction tests on day 11. In NAc, but not DS or mPFC, MOR mRNA decreased on abstinence day 1 and returned to basal levels over time. Naloxone significantly decreased cue-induced heroin seeking after 15 abstinence days but not 1 day.
Conclusions
Results suggest a role of MOR in incubation of heroin craving. As previous studies implicated NAc BDNF in incubation of cocaine craving, our data suggest that different mechanisms contribute to incubation of heroin versus cocaine craving.</description><identifier>ISSN: 0033-3158</identifier><identifier>EISSN: 1432-2072</identifier><identifier>DOI: 10.1007/s00213-012-2784-z</identifier><identifier>PMID: 22790874</identifier><identifier>CODEN: PSYPAG</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Behavioral decision theory ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Brain-Derived Neurotrophic Factor ; Cocaine ; Cues ; Dose-Response Relationship, Drug ; Drug addiction ; Gene Expression Regulation - drug effects ; Heroin ; Heroin - administration & dosage ; Heroin Dependence - psychology ; Male ; Medical sciences ; Messenger RNA ; Methyl-CpG-Binding Protein 2 - genetics ; Naloxone - administration & dosage ; Naloxone - pharmacology ; Narcotic Antagonists - administration & dosage ; Narcotic Antagonists - pharmacology ; Neurosciences ; Nucleus Accumbens - drug effects ; Nucleus Accumbens - metabolism ; Original Investigation ; Pharmacology/Toxicology ; Protein binding ; Psychiatry ; Psychopharmacology ; Rats ; Rats, Sprague-Dawley ; Receptor, trkB - genetics ; Receptors, Opioid, mu - genetics ; RNA, Messenger - metabolism ; Self Administration ; Time Factors</subject><ispartof>Psychopharmacologia, 2012-12, Vol.224 (4), p.559-571</ispartof><rights>Springer-Verlag (outside the USA) 2012</rights><rights>2014 INIST-CNRS</rights><rights>COPYRIGHT 2012 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2012</rights><rights>Springer-Verlag (outside the USA) 2012 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c600t-e40acaff047fe33d4f3b1ff54bc243dcea72122a5ded2f990a3e389fbb2ebe513</citedby><cites>FETCH-LOGICAL-c600t-e40acaff047fe33d4f3b1ff54bc243dcea72122a5ded2f990a3e389fbb2ebe513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00213-012-2784-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00213-012-2784-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26640586$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22790874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Theberge, Florence R. M.</creatorcontrib><creatorcontrib>Pickens, Charles L.</creatorcontrib><creatorcontrib>Goldart, Evan</creatorcontrib><creatorcontrib>Fanous, Sanya</creatorcontrib><creatorcontrib>Hope, Bruce T.</creatorcontrib><creatorcontrib>Liu, Qing-Rong</creatorcontrib><creatorcontrib>Shaham, Yavin</creatorcontrib><title>Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving</title><title>Psychopharmacologia</title><addtitle>Psychopharmacology</addtitle><addtitle>Psychopharmacology (Berl)</addtitle><description>Rationale and objectives
Responding to heroin cues progressively increases after cessation of heroin self-administration (incubation of heroin craving). We investigated whether this incubation is associated with time-dependent changes in brain-derived neurotrophic factor (BDNF) and methyl-CpG binding protein 2 (MeCP2) signaling and mu opioid receptor (MOR) expression in nucleus accumbens (NAc), dorsal striatum (DS), and medial prefrontal cortex (mPFC). We also investigated the effect of the preferential MOR antagonist naloxone on cue-induced heroin seeking during abstinence.
Methods
We trained rats to self-administer heroin or saline for 9–10 days and then dissected the NAc, DS, and mPFC at different abstinence days and measured mRNA and protein levels of BDNF, TrkB, and MeCP2, as well as MOR mRNA (
Oprm1
). In other groups, we assessed cue-induced heroin seeking in extinction tests after 1, 11, and 30 abstinence days, and naloxone’s (0–1.0 mg/kg) effect on extinction responding after 1 and 15 days.
Results
Cue-induced heroin seeking progressively increased or incubated during abstinence. This incubation was not associated with changes in BDNF, TrkB, or MeCP2 mRNA or protein levels in NAc, DS, or mPFC; additionally, no molecular changes were observed after extinction tests on day 11. In NAc, but not DS or mPFC, MOR mRNA decreased on abstinence day 1 and returned to basal levels over time. Naloxone significantly decreased cue-induced heroin seeking after 15 abstinence days but not 1 day.
Conclusions
Results suggest a role of MOR in incubation of heroin craving. As previous studies implicated NAc BDNF in incubation of cocaine craving, our data suggest that different mechanisms contribute to incubation of heroin versus cocaine craving.</description><subject>Animals</subject><subject>Behavioral decision theory</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain-Derived Neurotrophic Factor</subject><subject>Cocaine</subject><subject>Cues</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug addiction</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Heroin</subject><subject>Heroin - administration & dosage</subject><subject>Heroin Dependence - psychology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Messenger RNA</subject><subject>Methyl-CpG-Binding Protein 2 - genetics</subject><subject>Naloxone - administration & dosage</subject><subject>Naloxone - pharmacology</subject><subject>Narcotic Antagonists - administration & dosage</subject><subject>Narcotic Antagonists - pharmacology</subject><subject>Neurosciences</subject><subject>Nucleus Accumbens - drug effects</subject><subject>Nucleus Accumbens - metabolism</subject><subject>Original Investigation</subject><subject>Pharmacology/Toxicology</subject><subject>Protein binding</subject><subject>Psychiatry</subject><subject>Psychopharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptor, trkB - genetics</subject><subject>Receptors, Opioid, mu - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Self Administration</subject><subject>Time Factors</subject><issn>0033-3158</issn><issn>1432-2072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kt9u0zAUxiMEYmXwANwgSwiJi2b4X-r0BqkrDJDGQGhcW45z3HokdrCdAXsz3g6Xlm5D4FxYJ-f3fScn-oriMcFHBGPxImJMCSsxoSUVNS-v7hQTwlmusKB3iwnGjJWMVPVB8SDGC5wPr_n94oBSMce14JPi5yJGr61K1jvkDUq2h7KFAVwLLiG9Vm4FEVmH-hH5wXrbogAahuQD6j-dLaaoGRNyPqHjV2cnU3QevhxPUW6-h-VH-htByrVoCD5BtoHvQ4AYN-NyldaAgsr6UXcwRqS0HvsGXETfbFpnQo_N_tvWEHzW6KAurVs9LO4Z1UV4tLsPi88nr8-Xb8vTD2_eLRenpZ5hnErgWGllDObCAGMtN6whxlS80ZSzVoMSlFCqqhZaauZzrBiwem6ahkIDFWGHxcut7zA2PWSBS0F1cgi2V-GH9MrK2x1n13LlLyWr5gzzjcHznUHwX0eISfY2aug65cCPURJaCSEI4TyjT_9CL_wYXF5PEjIXpCaMVdfUSnUgrTM-z9UbU7lgFSd8xqjI1NE_qPy00FvtHRib398SkK1ABx9jALPfkWC5yZvc5k3mvMlN3uRV1jy5-XP2ij8By8CzHaCiVp0Jymkbr7nZjOOqnmWObrmYWzly4cbm_53-Cy0J738</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Theberge, Florence R. M.</creator><creator>Pickens, Charles L.</creator><creator>Goldart, Evan</creator><creator>Fanous, Sanya</creator><creator>Hope, Bruce T.</creator><creator>Liu, Qing-Rong</creator><creator>Shaham, Yavin</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20121201</creationdate><title>Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving</title><author>Theberge, Florence R. M. ; Pickens, Charles L. ; Goldart, Evan ; Fanous, Sanya ; Hope, Bruce T. ; Liu, Qing-Rong ; Shaham, Yavin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c600t-e40acaff047fe33d4f3b1ff54bc243dcea72122a5ded2f990a3e389fbb2ebe513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Behavioral decision theory</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brain-Derived Neurotrophic Factor</topic><topic>Cocaine</topic><topic>Cues</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug addiction</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Heroin</topic><topic>Heroin - administration & dosage</topic><topic>Heroin Dependence - psychology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Messenger RNA</topic><topic>Methyl-CpG-Binding Protein 2 - genetics</topic><topic>Naloxone - administration & dosage</topic><topic>Naloxone - pharmacology</topic><topic>Narcotic Antagonists - administration & dosage</topic><topic>Narcotic Antagonists - pharmacology</topic><topic>Neurosciences</topic><topic>Nucleus Accumbens - drug effects</topic><topic>Nucleus Accumbens - metabolism</topic><topic>Original Investigation</topic><topic>Pharmacology/Toxicology</topic><topic>Protein binding</topic><topic>Psychiatry</topic><topic>Psychopharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptor, trkB - genetics</topic><topic>Receptors, Opioid, mu - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Self Administration</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Theberge, Florence R. M.</creatorcontrib><creatorcontrib>Pickens, Charles L.</creatorcontrib><creatorcontrib>Goldart, Evan</creatorcontrib><creatorcontrib>Fanous, Sanya</creatorcontrib><creatorcontrib>Hope, Bruce T.</creatorcontrib><creatorcontrib>Liu, Qing-Rong</creatorcontrib><creatorcontrib>Shaham, Yavin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Psychopharmacologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Theberge, Florence R. M.</au><au>Pickens, Charles L.</au><au>Goldart, Evan</au><au>Fanous, Sanya</au><au>Hope, Bruce T.</au><au>Liu, Qing-Rong</au><au>Shaham, Yavin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving</atitle><jtitle>Psychopharmacologia</jtitle><stitle>Psychopharmacology</stitle><addtitle>Psychopharmacology (Berl)</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>224</volume><issue>4</issue><spage>559</spage><epage>571</epage><pages>559-571</pages><issn>0033-3158</issn><eissn>1432-2072</eissn><coden>PSYPAG</coden><abstract>Rationale and objectives
Responding to heroin cues progressively increases after cessation of heroin self-administration (incubation of heroin craving). We investigated whether this incubation is associated with time-dependent changes in brain-derived neurotrophic factor (BDNF) and methyl-CpG binding protein 2 (MeCP2) signaling and mu opioid receptor (MOR) expression in nucleus accumbens (NAc), dorsal striatum (DS), and medial prefrontal cortex (mPFC). We also investigated the effect of the preferential MOR antagonist naloxone on cue-induced heroin seeking during abstinence.
Methods
We trained rats to self-administer heroin or saline for 9–10 days and then dissected the NAc, DS, and mPFC at different abstinence days and measured mRNA and protein levels of BDNF, TrkB, and MeCP2, as well as MOR mRNA (
Oprm1
). In other groups, we assessed cue-induced heroin seeking in extinction tests after 1, 11, and 30 abstinence days, and naloxone’s (0–1.0 mg/kg) effect on extinction responding after 1 and 15 days.
Results
Cue-induced heroin seeking progressively increased or incubated during abstinence. This incubation was not associated with changes in BDNF, TrkB, or MeCP2 mRNA or protein levels in NAc, DS, or mPFC; additionally, no molecular changes were observed after extinction tests on day 11. In NAc, but not DS or mPFC, MOR mRNA decreased on abstinence day 1 and returned to basal levels over time. Naloxone significantly decreased cue-induced heroin seeking after 15 abstinence days but not 1 day.
Conclusions
Results suggest a role of MOR in incubation of heroin craving. As previous studies implicated NAc BDNF in incubation of cocaine craving, our data suggest that different mechanisms contribute to incubation of heroin versus cocaine craving.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22790874</pmid><doi>10.1007/s00213-012-2784-z</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Psychopharmacologia, 2012-12, Vol.224 (4), p.559-571 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Behavioral decision theory Biological and medical sciences Biomedical and Life Sciences Biomedicine Brain-Derived Neurotrophic Factor Cocaine Cues Dose-Response Relationship, Drug Drug addiction Gene Expression Regulation - drug effects Heroin Heroin - administration & dosage Heroin Dependence - psychology Male Medical sciences Messenger RNA Methyl-CpG-Binding Protein 2 - genetics Naloxone - administration & dosage Naloxone - pharmacology Narcotic Antagonists - administration & dosage Narcotic Antagonists - pharmacology Neurosciences Nucleus Accumbens - drug effects Nucleus Accumbens - metabolism Original Investigation Pharmacology/Toxicology Protein binding Psychiatry Psychopharmacology Rats Rats, Sprague-Dawley Receptor, trkB - genetics Receptors, Opioid, mu - genetics RNA, Messenger - metabolism Self Administration Time Factors |
| title | Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving |
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