Phosphorylation of hepatic AMP-activated protein kinase and liver kinase B1 is increased after a single oral dose of green tea extract to mice

Abstract We have previously shown that green and black tea extracts increase the phosphorylation of AMP-activated protein kinase (AMPK) and HMG-CoA reductase in rat hepatoma cells in culture, concomitant with a decrease in cholesterol synthesis. In the present study, we evaluated the ability of a si...

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Veröffentlicht in:Nutrition research (New York, N.Y.) N.Y.), 2012-12, Vol.32 (12), p.985-990
Hauptverfasser: Banerjee, Subhashis, Ghoshal, Sarbani, Porter, Todd D
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Ghoshal, Sarbani
Porter, Todd D
description Abstract We have previously shown that green and black tea extracts increase the phosphorylation of AMP-activated protein kinase (AMPK) and HMG-CoA reductase in rat hepatoma cells in culture, concomitant with a decrease in cholesterol synthesis. In the present study, we evaluated the ability of a single oral dose of green or black tea extract to promote the phosphorylation of AMPK, liver kinase B1 (LKB1, an AMPK-kinase), and HMG-CoA reductase in mouse liver. Green tea extract administered by gavage at 50 and 100 mg/kg caused a 2- to 3-fold increase in hepatic AMPK phosphorylation at 3 and 6 hours after dosing and a 1.5- to 2-fold increase in LKB1 phosphorylation at these same time points. The phosphorylation of HMG-CoA reductase at these and later time points was not significantly increased. Black tea administered by gavage at up to 250 mg/kg was ineffective in increasing hepatic AMPK phosphorylation. Both green and black tea extracts increased LKB1 phosphorylation in hepatoma cells in culture at 15 μ g/mL, and black tea also increased the phosphorylation of protein kinase A in hepatoma cells. These results suggest that compounds in both tea extracts activate AMPK by activating its upstream kinase, LKB1, and that black tea may do so by first activating protein kinase A, a known kinase for LKB1. Only green tea, at 50 and 100 mg/kg, was able to activate AMPK and LKB1 in mouse liver after oral dosing, suggesting that the polymerized catechins present in black tea do not reach the liver in sufficient concentration to affect AMPK activity.
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In the present study, we evaluated the ability of a single oral dose of green or black tea extract to promote the phosphorylation of AMPK, liver kinase B1 (LKB1, an AMPK-kinase), and HMG-CoA reductase in mouse liver. Green tea extract administered by gavage at 50 and 100 mg/kg caused a 2- to 3-fold increase in hepatic AMPK phosphorylation at 3 and 6 hours after dosing and a 1.5- to 2-fold increase in LKB1 phosphorylation at these same time points. The phosphorylation of HMG-CoA reductase at these and later time points was not significantly increased. Black tea administered by gavage at up to 250 mg/kg was ineffective in increasing hepatic AMPK phosphorylation. Both green and black tea extracts increased LKB1 phosphorylation in hepatoma cells in culture at 15 μ g/mL, and black tea also increased the phosphorylation of protein kinase A in hepatoma cells. 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Feeding behavior</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastroenterology and Hepatology</subject><subject>Green tea</subject><subject>Hepatoma cell</subject><subject>Hydroxymethylglutaryl CoA Reductases - metabolism</subject><subject>Hypercholesterolemia - metabolism</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>LKB1</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mouse</subject><subject>Phosphorylation</subject><subject>Plant Extracts - pharmacology</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Rats</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0271-5317</issn><issn>1879-0739</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUsmOEzEQbSEQEwb-ACFfOHbw3t0XpGHEJg1iJOBsOXY5caZjt2wnIj_BN-NWZgEunMq1vVflV03zkuAlwUS-2S7DviTIS4oJraElxuJRsyB9N7S4Y8PjZoFpR1rBSHfWPMt5izHpCGNPmzPKKOeC80Xz63oT87SJ6Tjq4mNA0aENTPVt0MWX61ab4g-6gEVTigV8QDc-6AxIB4tGf4B0F3hHkM_IB5OguhZpV2pSo-zDegQUkx6RjbWwMqwTQEAFNIKfJVUOVCLaeQPPmydOjxle3Nrz5seH998vP7VXXz9-vry4ao0kvLTApOg4d06sBHG0ur2ry2HC5YD7HpizvFpLB6ktXfUWBnBs0FQaKbg07Lx5e8Kd9qsdWAOhjjGqKfmdTkcVtVd_Z4LfqHU8KCYqAcUVgJ8ATIo5J3D3vQSrWR-1VSd91KzPHK361LZXf_LeN90JUgte3xbobPTokg7G54c62QkimHxYAOovHTwklY2HYMD6BKYoG_3_JvkXwIw--Mp5A0fI27hPoSqgiMpUYfVtvqX5lAjFmBLJ2G-Ltcfn</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Banerjee, Subhashis</creator><creator>Ghoshal, Sarbani</creator><creator>Porter, Todd D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20121201</creationdate><title>Phosphorylation of hepatic AMP-activated protein kinase and liver kinase B1 is increased after a single oral dose of green tea extract to mice</title><author>Banerjee, Subhashis ; Ghoshal, Sarbani ; Porter, Todd D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c614t-e365744ff5b51f2e368f01701469088e3fd4088d296ad2b8de9ef39a26c6546c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Administration, Oral</topic><topic>AMP-Activated Protein Kinases - metabolism</topic><topic>AMP-kinase</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Black tea</topic><topic>Camellia sinensis - chemistry</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Catechin - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>Feeding. Feeding behavior</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastroenterology and Hepatology</topic><topic>Green tea</topic><topic>Hepatoma cell</topic><topic>Hydroxymethylglutaryl CoA Reductases - metabolism</topic><topic>Hypercholesterolemia - metabolism</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>LKB1</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mouse</topic><topic>Phosphorylation</topic><topic>Plant Extracts - pharmacology</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Rats</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banerjee, Subhashis</creatorcontrib><creatorcontrib>Ghoshal, Sarbani</creatorcontrib><creatorcontrib>Porter, Todd D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nutrition research (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banerjee, Subhashis</au><au>Ghoshal, Sarbani</au><au>Porter, Todd D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphorylation of hepatic AMP-activated protein kinase and liver kinase B1 is increased after a single oral dose of green tea extract to mice</atitle><jtitle>Nutrition research (New York, N.Y.)</jtitle><addtitle>Nutr Res</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>32</volume><issue>12</issue><spage>985</spage><epage>990</epage><pages>985-990</pages><issn>0271-5317</issn><eissn>1879-0739</eissn><coden>NTRSDC</coden><abstract>Abstract We have previously shown that green and black tea extracts increase the phosphorylation of AMP-activated protein kinase (AMPK) and HMG-CoA reductase in rat hepatoma cells in culture, concomitant with a decrease in cholesterol synthesis. 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These results suggest that compounds in both tea extracts activate AMPK by activating its upstream kinase, LKB1, and that black tea may do so by first activating protein kinase A, a known kinase for LKB1. Only green tea, at 50 and 100 mg/kg, was able to activate AMPK and LKB1 in mouse liver after oral dosing, suggesting that the polymerized catechins present in black tea do not reach the liver in sufficient concentration to affect AMPK activity.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>23244544</pmid><doi>10.1016/j.nutres.2012.10.005</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects Administration, Oral
AMP-Activated Protein Kinases - metabolism
AMP-kinase
Animals
Biological and medical sciences
Black tea
Camellia sinensis - chemistry
Carcinoma, Hepatocellular - metabolism
Catechin - pharmacology
Cell Line, Tumor
Cyclic AMP-Dependent Protein Kinases - metabolism
Feeding. Feeding behavior
Female
Fundamental and applied biological sciences. Psychology
Gastroenterology and Hepatology
Green tea
Hepatoma cell
Hydroxymethylglutaryl CoA Reductases - metabolism
Hypercholesterolemia - metabolism
Liver
Liver - metabolism
LKB1
Mice
Mice, Inbred C57BL
Mouse
Phosphorylation
Plant Extracts - pharmacology
Protein-Serine-Threonine Kinases - metabolism
Rats
Vertebrates: anatomy and physiology, studies on body, several organs or systems
title Phosphorylation of hepatic AMP-activated protein kinase and liver kinase B1 is increased after a single oral dose of green tea extract to mice
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