Enrichment of Plasma Membrane Proteins Using Nanoparticle Pellicles: Comparison between Silica and Higher Density Nanoparticles
Proteomic and other characterization of plasma membrane proteins is made difficult by their low abundance, hydrophobicity, frequent carboxylation, and dynamic population. We and others have proposed that underrepresentation in LC-MS/MS analysis can be partially compensated by enriching the plasma me...
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| Veröffentlicht in: | Journal of proteome research 2013-03, Vol.12 (3), p.1134-1141 |
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| container_title | Journal of proteome research |
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| creator | Choksawangkarn, Waeowalee Kim, Sung-Kyoung Cannon, Joe R Edwards, Nathan J Lee, Sang Bok Fenselau, Catherine |
| description | Proteomic and other characterization of plasma membrane proteins is made difficult by their low abundance, hydrophobicity, frequent carboxylation, and dynamic population. We and others have proposed that underrepresentation in LC-MS/MS analysis can be partially compensated by enriching the plasma membrane and its proteins using cationic nanoparticle pellicles. The nanoparticles increase the density of plasma membrane sheets and thus enhance separation by centrifugation from other lysed cellular components. Herein, we test the hypothesis that the use of nanoparticles with increased densities can provide enhanced enrichment of plasma membrane proteins for proteomic analysis. Multiple myeloma cells were grown and coated in suspension with three different pellicles of three different densities and both pellicle coated and uncoated suspensions analyzed by high-throughput LC-MS/MS. Enrichment was evaluated by the total number and the spectral counts of identified plasma membrane proteins. |
| doi_str_mv | 10.1021/pr301107x |
| format | Article |
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We and others have proposed that underrepresentation in LC-MS/MS analysis can be partially compensated by enriching the plasma membrane and its proteins using cationic nanoparticle pellicles. The nanoparticles increase the density of plasma membrane sheets and thus enhance separation by centrifugation from other lysed cellular components. Herein, we test the hypothesis that the use of nanoparticles with increased densities can provide enhanced enrichment of plasma membrane proteins for proteomic analysis. Multiple myeloma cells were grown and coated in suspension with three different pellicles of three different densities and both pellicle coated and uncoated suspensions analyzed by high-throughput LC-MS/MS. 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Proteome Res</addtitle><description>Proteomic and other characterization of plasma membrane proteins is made difficult by their low abundance, hydrophobicity, frequent carboxylation, and dynamic population. We and others have proposed that underrepresentation in LC-MS/MS analysis can be partially compensated by enriching the plasma membrane and its proteins using cationic nanoparticle pellicles. The nanoparticles increase the density of plasma membrane sheets and thus enhance separation by centrifugation from other lysed cellular components. Herein, we test the hypothesis that the use of nanoparticles with increased densities can provide enhanced enrichment of plasma membrane proteins for proteomic analysis. Multiple myeloma cells were grown and coated in suspension with three different pellicles of three different densities and both pellicle coated and uncoated suspensions analyzed by high-throughput LC-MS/MS. 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Proteome Res</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>12</volume><issue>3</issue><spage>1134</spage><epage>1141</epage><pages>1134-1141</pages><issn>1535-3893</issn><issn>1535-3907</issn><eissn>1535-3907</eissn><abstract>Proteomic and other characterization of plasma membrane proteins is made difficult by their low abundance, hydrophobicity, frequent carboxylation, and dynamic population. We and others have proposed that underrepresentation in LC-MS/MS analysis can be partially compensated by enriching the plasma membrane and its proteins using cationic nanoparticle pellicles. The nanoparticles increase the density of plasma membrane sheets and thus enhance separation by centrifugation from other lysed cellular components. Herein, we test the hypothesis that the use of nanoparticles with increased densities can provide enhanced enrichment of plasma membrane proteins for proteomic analysis. 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| ispartof | Journal of proteome research, 2013-03, Vol.12 (3), p.1134-1141 |
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| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Blotting, Western carboxylation Cell Line, Tumor Centrifugation Chromatography, Liquid Humans hydrophobicity membrane proteins Membrane Proteins - metabolism Microscopy, Electron, Transmission Multiple Myeloma - metabolism Multiple Myeloma - pathology myeloma Nanoparticles plasma membrane proteome proteomics silica Silicon Dioxide Tandem Mass Spectrometry |
| title | Enrichment of Plasma Membrane Proteins Using Nanoparticle Pellicles: Comparison between Silica and Higher Density Nanoparticles |
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