Post-Polyketide Synthase Steps in Iso-migrastatin Biosynthesis, Featuring Tailoring Enzymes with Broad Substrate Specificity

The iso-migrastatin (iso-MGS) biosynthetic gene cluster from Streptomyces platensis NRRL 18993 consists of 11 genes, featuring an acyltransferase (AT)-less type I polyketide synthase (PKS) and three tailoring enzymes MgsIJK. Systematic inactivation of mgsIJK in S. platensis enabled us to (i) ide...

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Veröffentlicht in:	Journal of the American Chemical Society 2013-02, Vol.135 (7), p.2489-2492
Hauptverfasser:	Ma, Ming, Kwong, Thomas, Lim, Si-Kyu, Ju, Jianhua, Lohman, Jeremy R, Shen, Ben
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosynthetic Pathways Macrolides - chemistry Molecular Structure Piperidones - chemistry Polyketide Synthases - chemistry Streptomyces - enzymology Streptomyces - genetics Substrate Specificity
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container_title	Journal of the American Chemical Society
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creator	Ma, Ming Kwong, Thomas Lim, Si-Kyu Ju, Jianhua Lohman, Jeremy R Shen, Ben
description	The iso-migrastatin (iso-MGS) biosynthetic gene cluster from Streptomyces platensis NRRL 18993 consists of 11 genes, featuring an acyltransferase (AT)-less type I polyketide synthase (PKS) and three tailoring enzymes MgsIJK. Systematic inactivation of mgsIJK in S. platensis enabled us to (i) identify two nascent products of the iso-MGS AT-less type I PKS, establishing an unprecedented novel feature for AT-less type I PKSs, and (ii) account for the formation of all known post-PKS biosynthetic intermediates generated by the three tailoring enzymes MgsIJK, which possessed significant substrate promiscuities.
doi_str_mv	10.1021/ja4002635
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Am. Chem. Soc</addtitle><date>2013-02-20</date><risdate>2013</risdate><volume>135</volume><issue>7</issue><spage>2489</spage><epage>2492</epage><pages>2489-2492</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><abstract>The iso-migrastatin (iso-MGS) biosynthetic gene cluster from Streptomyces platensis NRRL 18993 consists of 11 genes, featuring an acyltransferase (AT)-less type I polyketide synthase (PKS) and three tailoring enzymes MgsIJK. 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source	MEDLINE; ACS Publications
subjects	Biosynthetic Pathways Macrolides - chemistry Molecular Structure Piperidones - chemistry Polyketide Synthases - chemistry Streptomyces - enzymology Streptomyces - genetics Substrate Specificity
title	Post-Polyketide Synthase Steps in Iso-migrastatin Biosynthesis, Featuring Tailoring Enzymes with Broad Substrate Specificity
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