Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells

Mouse embryonic stem cell (ESC) lines, and more recently human ESC lines, have become valuable tools for studying early mammalian development. Increasing interest in ESCs and their differentiated progeny in drug discovery and as potential therapeutic agents has highlighted the fact that current two-...

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Veröffentlicht in:	Biotechnology and bioengineering 2010-11, Vol.107 (4), p.683-695
Hauptverfasser:	Storm, Michael P, Orchard, Craig B, Bone, Heather K, Chaudhuri, Julian B, Welham, Melanie J
Format:	Artikel
Sprache:	eng
Schlagworte:	3D agitated suspension culture Animal cells Animals Biological and medical sciences Bioreactors Biotechnology Biotechnology - methods Cell culture Cell Culture Techniques - methods Embryology Embryonic Stem Cells Embryos Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Humans Mammals Methods. Procedures. Technologies Mice microcarriers Microspheres pluripotency Stem cells
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