Pollination induces autophagy in petunia petals via ethylene
Autophagy is one of the main mechanisms of degradation and remobilization of macromolecules, and it appears to play an important role in petal senescence. However, little is known about the regulatory mechanisms of autophagy in petal senescence. Autophagic processes were observed by electron microsc...
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| Veröffentlicht in: | Journal of experimental botany 2013-02, Vol.64 (4), p.1111-1120 |
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| creator | Shibuya, Kenichi Niki , Tomoko Ichimura, Kazuo |
| description | Autophagy is one of the main mechanisms of degradation and remobilization of macromolecules, and it appears to play an important role in petal senescence. However, little is known about the regulatory mechanisms of autophagy in petal senescence. Autophagic processes were observed by electron microscopy and monodansylcadaverine staining of senescing petals of petunia (Petunia hybrida); autophagy-related gene 8 (ATG8) homologues were isolated from petunia and the regulation of expression was analysed. Nutrient remobilization was also examined during pollination-induced petal senescence. Active autophagic processes were observed in the mesophyll cells of senescing petunia petals. Pollination induced the expression of PhATG8 homologues and was accompanied by an increase in ethylene production. Ethylene inhibitor treatment in pollinated flowers delayed the induction of PhATG8 homologues, and ethylene treatment rapidly upregulated PhATG8 homologues in petunia petals. Dry weight and nitrogen content were decreased in the petals and increased in the ovaries after pollination in detached flowers. These results indicated that pollination induces autophagy and that ethylene is a key regulator of autophagy in petal senescence of petunia. The data also demonstrated the translocation of nutrients from the petals to the ovaries during pollination-induced petal senescence. |
| doi_str_mv | 10.1093/jxb/ers395 |
| format | Article |
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However, little is known about the regulatory mechanisms of autophagy in petal senescence. Autophagic processes were observed by electron microscopy and monodansylcadaverine staining of senescing petals of petunia (Petunia hybrida); autophagy-related gene 8 (ATG8) homologues were isolated from petunia and the regulation of expression was analysed. Nutrient remobilization was also examined during pollination-induced petal senescence. Active autophagic processes were observed in the mesophyll cells of senescing petunia petals. Pollination induced the expression of PhATG8 homologues and was accompanied by an increase in ethylene production. Ethylene inhibitor treatment in pollinated flowers delayed the induction of PhATG8 homologues, and ethylene treatment rapidly upregulated PhATG8 homologues in petunia petals. Dry weight and nitrogen content were decreased in the petals and increased in the ovaries after pollination in detached flowers. These results indicated that pollination induces autophagy and that ethylene is a key regulator of autophagy in petal senescence of petunia. The data also demonstrated the translocation of nutrients from the petals to the ovaries during pollination-induced petal senescence.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/ers395</identifier><identifier>PMID: 23349142</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press [etc.]</publisher><subject><![CDATA[analogs & derivatives ; anatomy & histology ; antagonists & inhibitors ; Autophagy ; Biological and medical sciences ; Biological Transport ; Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation ; Cadaverine ; Cadaverine - analogs & derivatives ; Cadaverine - metabolism ; Cellular senescence ; corolla ; electron microscopy ; ethylene ; ethylene inhibitors ; Ethylene production ; Ethylenes ; Ethylenes - antagonists & inhibitors ; Ethylenes - metabolism ; Flowers ; Flowers - genetics ; Flowers - metabolism ; Flowers - ultrastructure ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; genes ; Genes, Plant ; genetics ; mesophyll ; Mesophyll Cells ; Mesophyll Cells - metabolism ; Messenger RNA ; metabolism ; Microscopy, Electron, Transmission ; Multigene Family ; Nitrogen ; nitrogen content ; nutrients ; Ovaries ; Petals ; Petunia ; Petunia - anatomy & histology ; Petunia - genetics ; Petunia - metabolism ; Petunia hybrida ; Plant Cells ; Plant Cells - metabolism ; plant ovary ; Plant physiology and development ; Plant Proteins ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants ; Pollination ; RESEARCH PAPER ; RNA, Messenger ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Plant ; RNA, Plant - genetics ; RNA, Plant - metabolism ; senescence ; Senescence and abscission ; ultrastructure ; Up-Regulation ; Vegetative and sexual reproduction, floral biology, fructification ; Vegetative apparatus, growth and morphogenesis. Senescence]]></subject><ispartof>Journal of experimental botany, 2013-02, Vol.64 (4), p.1111-1120</ispartof><rights>Society for Experimental Biology 2013</rights><rights>2014 INIST-CNRS</rights><rights>The Authors [2013]. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c553t-f499d422d923442dcaa53f3968a5b37917ec336f2fd4f0dc1d9437aa0ab359fd3</citedby><cites>FETCH-LOGICAL-c553t-f499d422d923442dcaa53f3968a5b37917ec336f2fd4f0dc1d9437aa0ab359fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24040874$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24040874$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27157745$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23349142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shibuya, Kenichi</creatorcontrib><creatorcontrib>Niki , Tomoko</creatorcontrib><creatorcontrib>Ichimura, Kazuo</creatorcontrib><title>Pollination induces autophagy in petunia petals via ethylene</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>Autophagy is one of the main mechanisms of degradation and remobilization of macromolecules, and it appears to play an important role in petal senescence. However, little is known about the regulatory mechanisms of autophagy in petal senescence. Autophagic processes were observed by electron microscopy and monodansylcadaverine staining of senescing petals of petunia (Petunia hybrida); autophagy-related gene 8 (ATG8) homologues were isolated from petunia and the regulation of expression was analysed. Nutrient remobilization was also examined during pollination-induced petal senescence. Active autophagic processes were observed in the mesophyll cells of senescing petunia petals. Pollination induced the expression of PhATG8 homologues and was accompanied by an increase in ethylene production. Ethylene inhibitor treatment in pollinated flowers delayed the induction of PhATG8 homologues, and ethylene treatment rapidly upregulated PhATG8 homologues in petunia petals. Dry weight and nitrogen content were decreased in the petals and increased in the ovaries after pollination in detached flowers. These results indicated that pollination induces autophagy and that ethylene is a key regulator of autophagy in petal senescence of petunia. The data also demonstrated the translocation of nutrients from the petals to the ovaries during pollination-induced petal senescence.</description><subject>analogs & derivatives</subject><subject>anatomy & histology</subject><subject>antagonists & inhibitors</subject><subject>Autophagy</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation</subject><subject>Cadaverine</subject><subject>Cadaverine - analogs & derivatives</subject><subject>Cadaverine - metabolism</subject><subject>Cellular senescence</subject><subject>corolla</subject><subject>electron microscopy</subject><subject>ethylene</subject><subject>ethylene inhibitors</subject><subject>Ethylene production</subject><subject>Ethylenes</subject><subject>Ethylenes - antagonists & inhibitors</subject><subject>Ethylenes - metabolism</subject><subject>Flowers</subject><subject>Flowers - genetics</subject><subject>Flowers - metabolism</subject><subject>Flowers - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>genetics</subject><subject>mesophyll</subject><subject>Mesophyll Cells</subject><subject>Mesophyll Cells - metabolism</subject><subject>Messenger RNA</subject><subject>metabolism</subject><subject>Microscopy, Electron, Transmission</subject><subject>Multigene Family</subject><subject>Nitrogen</subject><subject>nitrogen content</subject><subject>nutrients</subject><subject>Ovaries</subject><subject>Petals</subject><subject>Petunia</subject><subject>Petunia - anatomy & histology</subject><subject>Petunia - genetics</subject><subject>Petunia - metabolism</subject><subject>Petunia hybrida</subject><subject>Plant Cells</subject><subject>Plant Cells - metabolism</subject><subject>plant ovary</subject><subject>Plant physiology and development</subject><subject>Plant Proteins</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>Pollination</subject><subject>RESEARCH PAPER</subject><subject>RNA, Messenger</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Plant</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - metabolism</subject><subject>senescence</subject><subject>Senescence and abscission</subject><subject>ultrastructure</subject><subject>Up-Regulation</subject><subject>Vegetative and sexual reproduction, floral biology, fructification</subject><subject>Vegetative apparatus, growth and morphogenesis. Senescence</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2LFDEQxYMo7rh68a7ORRCh3SSVdHdABFn8ggUF3XOoycdMhp5kTLoX5783Q4-7evNURb0fj6p6hDxl9A2jCi62v1YXLhdQ8h5ZMNHShgtg98mCUs4bqmR3Rh6VsqWUSirlQ3LGAYRigi_I229pGELEMaS4DNFOxpUlTmPab3B9qJPl3o1TDHisOJTlTW3duDkMLrrH5IGvM_fkVM_J9ccPPy4_N1dfP325fH_VGClhbLxQygrOreIgBLcGUYIH1fYoV9Ap1jkD0HrurfDUGmaVgA6R4gqk8hbOybvZdz-tds4aF8eMg97nsMN80AmD_leJYaPX6UaD7GnPWTV4dTLI6efkyqh3oRg3DBhdmopmbVvRnvX_gQLjPRyfV9HXM2pyKiU7f7sRo_qYjK7J6DmZCj__-4Zb9E8UFXh5ArAYHHzGaEK54zomu04cjZ7N3LaMKd_pggrad6LqL2bdY9K4ztXj-junTNb4WVf_DL8BF1KqwA</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Shibuya, Kenichi</creator><creator>Niki , Tomoko</creator><creator>Ichimura, Kazuo</creator><general>Oxford University Press [etc.]</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20130201</creationdate><title>Pollination induces autophagy in petunia petals via ethylene</title><author>Shibuya, Kenichi ; Niki , Tomoko ; Ichimura, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c553t-f499d422d923442dcaa53f3968a5b37917ec336f2fd4f0dc1d9437aa0ab359fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>analogs & derivatives</topic><topic>anatomy & histology</topic><topic>antagonists & inhibitors</topic><topic>Autophagy</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation</topic><topic>Cadaverine</topic><topic>Cadaverine - analogs & derivatives</topic><topic>Cadaverine - metabolism</topic><topic>Cellular senescence</topic><topic>corolla</topic><topic>electron microscopy</topic><topic>ethylene</topic><topic>ethylene inhibitors</topic><topic>Ethylene production</topic><topic>Ethylenes</topic><topic>Ethylenes - antagonists & inhibitors</topic><topic>Ethylenes - metabolism</topic><topic>Flowers</topic><topic>Flowers - genetics</topic><topic>Flowers - metabolism</topic><topic>Flowers - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>genetics</topic><topic>mesophyll</topic><topic>Mesophyll Cells</topic><topic>Mesophyll Cells - metabolism</topic><topic>Messenger RNA</topic><topic>metabolism</topic><topic>Microscopy, Electron, Transmission</topic><topic>Multigene Family</topic><topic>Nitrogen</topic><topic>nitrogen content</topic><topic>nutrients</topic><topic>Ovaries</topic><topic>Petals</topic><topic>Petunia</topic><topic>Petunia - anatomy & histology</topic><topic>Petunia - genetics</topic><topic>Petunia - metabolism</topic><topic>Petunia hybrida</topic><topic>Plant Cells</topic><topic>Plant Cells - metabolism</topic><topic>plant ovary</topic><topic>Plant physiology and development</topic><topic>Plant Proteins</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants</topic><topic>Pollination</topic><topic>RESEARCH PAPER</topic><topic>RNA, Messenger</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Plant</topic><topic>RNA, Plant - genetics</topic><topic>RNA, Plant - metabolism</topic><topic>senescence</topic><topic>Senescence and abscission</topic><topic>ultrastructure</topic><topic>Up-Regulation</topic><topic>Vegetative and sexual reproduction, floral biology, fructification</topic><topic>Vegetative apparatus, growth and morphogenesis. Senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shibuya, Kenichi</creatorcontrib><creatorcontrib>Niki , Tomoko</creatorcontrib><creatorcontrib>Ichimura, Kazuo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shibuya, Kenichi</au><au>Niki , Tomoko</au><au>Ichimura, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pollination induces autophagy in petunia petals via ethylene</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J Exp Bot</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>64</volume><issue>4</issue><spage>1111</spage><epage>1120</epage><pages>1111-1120</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>Autophagy is one of the main mechanisms of degradation and remobilization of macromolecules, and it appears to play an important role in petal senescence. However, little is known about the regulatory mechanisms of autophagy in petal senescence. Autophagic processes were observed by electron microscopy and monodansylcadaverine staining of senescing petals of petunia (Petunia hybrida); autophagy-related gene 8 (ATG8) homologues were isolated from petunia and the regulation of expression was analysed. Nutrient remobilization was also examined during pollination-induced petal senescence. Active autophagic processes were observed in the mesophyll cells of senescing petunia petals. Pollination induced the expression of PhATG8 homologues and was accompanied by an increase in ethylene production. Ethylene inhibitor treatment in pollinated flowers delayed the induction of PhATG8 homologues, and ethylene treatment rapidly upregulated PhATG8 homologues in petunia petals. Dry weight and nitrogen content were decreased in the petals and increased in the ovaries after pollination in detached flowers. These results indicated that pollination induces autophagy and that ethylene is a key regulator of autophagy in petal senescence of petunia. The data also demonstrated the translocation of nutrients from the petals to the ovaries during pollination-induced petal senescence.</abstract><cop>Oxford</cop><pub>Oxford University Press [etc.]</pub><pmid>23349142</pmid><doi>10.1093/jxb/ers395</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | analogs & derivatives anatomy & histology antagonists & inhibitors Autophagy Biological and medical sciences Biological Transport Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation Cadaverine Cadaverine - analogs & derivatives Cadaverine - metabolism Cellular senescence corolla electron microscopy ethylene ethylene inhibitors Ethylene production Ethylenes Ethylenes - antagonists & inhibitors Ethylenes - metabolism Flowers Flowers - genetics Flowers - metabolism Flowers - ultrastructure Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant genes Genes, Plant genetics mesophyll Mesophyll Cells Mesophyll Cells - metabolism Messenger RNA metabolism Microscopy, Electron, Transmission Multigene Family Nitrogen nitrogen content nutrients Ovaries Petals Petunia Petunia - anatomy & histology Petunia - genetics Petunia - metabolism Petunia hybrida Plant Cells Plant Cells - metabolism plant ovary Plant physiology and development Plant Proteins Plant Proteins - genetics Plant Proteins - metabolism Plants Pollination RESEARCH PAPER RNA, Messenger RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Plant RNA, Plant - genetics RNA, Plant - metabolism senescence Senescence and abscission ultrastructure Up-Regulation Vegetative and sexual reproduction, floral biology, fructification Vegetative apparatus, growth and morphogenesis. Senescence |
| title | Pollination induces autophagy in petunia petals via ethylene |
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