A malachite green-based assay to assess glucan phosphatase activity

With the recent discovery of a unique class of dual-specificity phosphatases that dephosphorylate glucans, we report an in vitro assay tailored for the detection of phosphatase activity against phosphorylated glucans. We demonstrate that, in contrast to a general phosphatase assay using a synthetic...

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Veröffentlicht in:	Analytical biochemistry 2013-04, Vol.435 (1), p.54-56
Hauptverfasser:	Sherwood, Amanda R., Paasch, Bradley C., Worby, Carolyn A., Gentry, Matthew S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amylopectin Amylopectin - metabolism Animals Arabidopsis - enzymology cost effectiveness Dual-Specificity Phosphatases - metabolism Enzyme Assays - economics Enzyme Assays - methods Glucan Glycogen Glycogen - metabolism Humans in vitro studies malachite Malachite green Phosphatase phosphates Phosphorylation Rabbits Rosaniline Dyes - analysis Rosaniline Dyes - metabolism Starch Starch - metabolism Substrate Specificity
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