A Novel Porous Gelatin Composite Containing Naringin for Bone Repair
As Gu-Sui-Bu (GSB) is a commonly used Chinese medical herb for therapeutic treatment of bone-related diseases, naringin is its main active component. This study elucidates how various concentrations of naringin solution affect the activities of bone cells, based on colorimetric, alkaline phosphatase...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-10 |
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| creator | Chen, Kuo-Yu Lin, Kuen-Cherng Chen, Yueh-Sheng Yao, Chun-Hsu |
| description | As Gu-Sui-Bu (GSB) is a commonly used Chinese medical herb for therapeutic treatment of bone-related diseases, naringin is its main active component. This study elucidates how various concentrations of naringin solution affect the activities of bone cells, based on colorimetric, alkaline phosphatase activity, nodule formation, and tartrate-resistant acid phosphatase activity assays to determine the optimal concentration of naringin. GGT composite was obtained by combining genipin cross-linked gelatin and β-tricalcium phosphate. GGTN composite was prepared by mixing GGT composite with the predetermined concentration of naringin. Porous GGT and GGTN composites were then made using a salt-leaching procedure. The potential of the composites in repairing bone defects was evaluated and compared in vivo by using the biological response of rabbit calvarial bone to these composites. Consequently, the most effective concentration of naringin was 10 mg/mL, which significantly enhanced the proliferation of osteoblasts, osteoclast activity, and nodule formation without affecting the alkaline phosphatase activity of osteoblasts and mitochondrial activity of mixed-bone cells. Radiographic analysis revealed greater new bone ingrowth in the GGTN composite than in the GGT composite at the same implantation time. Therefore, the GGTN composite is highly promising for use as a bone graft material. |
| doi_str_mv | 10.1155/2013/283941 |
| format | Article |
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This study elucidates how various concentrations of naringin solution affect the activities of bone cells, based on colorimetric, alkaline phosphatase activity, nodule formation, and tartrate-resistant acid phosphatase activity assays to determine the optimal concentration of naringin. GGT composite was obtained by combining genipin cross-linked gelatin and β-tricalcium phosphate. GGTN composite was prepared by mixing GGT composite with the predetermined concentration of naringin. Porous GGT and GGTN composites were then made using a salt-leaching procedure. The potential of the composites in repairing bone defects was evaluated and compared in vivo by using the biological response of rabbit calvarial bone to these composites. Consequently, the most effective concentration of naringin was 10 mg/mL, which significantly enhanced the proliferation of osteoblasts, osteoclast activity, and nodule formation without affecting the alkaline phosphatase activity of osteoblasts and mitochondrial activity of mixed-bone cells. Radiographic analysis revealed greater new bone ingrowth in the GGTN composite than in the GGT composite at the same implantation time. Therefore, the GGTN composite is highly promising for use as a bone graft material.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2013/283941</identifier><identifier>PMID: 23431335</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Acid phosphatase ; Acid phosphatase (tartrate-resistant) ; Acid resistance ; Alkaline phosphatase ; Biodegradation ; Biomedical materials ; Bone cancer ; Bone grafts ; Bone healing ; Calcium phosphates ; Chinese medicine ; Citrus fruits ; Colorimetry ; Diabetes ; Flavonoids ; Gelatin ; Genipin ; Grafting ; Growth factors ; Implantation ; Laboratory animals ; Leaching ; Medical treatment ; Mitochondria ; Osteoblasts ; Osteoporosis ; Phosphatase ; R&D ; Research & development ; Substitute bone ; Tricalcium phosphate</subject><ispartof>Evidence-based complementary and alternative medicine, 2013-01, Vol.2013 (2013), p.1-10</ispartof><rights>Copyright © 2013 Kuo-Yu Chen et al.</rights><rights>Copyright © 2013 Kuo-Yu Chen et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2013 Kuo-Yu Chen et al. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-1eee30c5327eb8bf7a91d9db595dde9147a6df82b7d11bb7f144ebfe6aa373733</citedby><cites>FETCH-LOGICAL-c532t-1eee30c5327eb8bf7a91d9db595dde9147a6df82b7d11bb7f144ebfe6aa373733</cites><orcidid>0000-0002-1654-2715</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575669/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575669/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23431335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wang, Chong-Zhi</contributor><creatorcontrib>Chen, Kuo-Yu</creatorcontrib><creatorcontrib>Lin, Kuen-Cherng</creatorcontrib><creatorcontrib>Chen, Yueh-Sheng</creatorcontrib><creatorcontrib>Yao, Chun-Hsu</creatorcontrib><title>A Novel Porous Gelatin Composite Containing Naringin for Bone Repair</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>As Gu-Sui-Bu (GSB) is a commonly used Chinese medical herb for therapeutic treatment of bone-related diseases, naringin is its main active component. This study elucidates how various concentrations of naringin solution affect the activities of bone cells, based on colorimetric, alkaline phosphatase activity, nodule formation, and tartrate-resistant acid phosphatase activity assays to determine the optimal concentration of naringin. GGT composite was obtained by combining genipin cross-linked gelatin and β-tricalcium phosphate. GGTN composite was prepared by mixing GGT composite with the predetermined concentration of naringin. Porous GGT and GGTN composites were then made using a salt-leaching procedure. The potential of the composites in repairing bone defects was evaluated and compared in vivo by using the biological response of rabbit calvarial bone to these composites. Consequently, the most effective concentration of naringin was 10 mg/mL, which significantly enhanced the proliferation of osteoblasts, osteoclast activity, and nodule formation without affecting the alkaline phosphatase activity of osteoblasts and mitochondrial activity of mixed-bone cells. Radiographic analysis revealed greater new bone ingrowth in the GGTN composite than in the GGT composite at the same implantation time. Therefore, the GGTN composite is highly promising for use as a bone graft material.</description><subject>Acid phosphatase</subject><subject>Acid phosphatase (tartrate-resistant)</subject><subject>Acid resistance</subject><subject>Alkaline phosphatase</subject><subject>Biodegradation</subject><subject>Biomedical materials</subject><subject>Bone cancer</subject><subject>Bone grafts</subject><subject>Bone healing</subject><subject>Calcium phosphates</subject><subject>Chinese medicine</subject><subject>Citrus fruits</subject><subject>Colorimetry</subject><subject>Diabetes</subject><subject>Flavonoids</subject><subject>Gelatin</subject><subject>Genipin</subject><subject>Grafting</subject><subject>Growth factors</subject><subject>Implantation</subject><subject>Laboratory animals</subject><subject>Leaching</subject><subject>Medical treatment</subject><subject>Mitochondria</subject><subject>Osteoblasts</subject><subject>Osteoporosis</subject><subject>Phosphatase</subject><subject>R&D</subject><subject>Research & development</subject><subject>Substitute bone</subject><subject>Tricalcium phosphate</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc1LxDAQxYMofp88KwUvoqx2kqZpLoKunyAqouAtpNupRrrJmnQV_3tTqot6kTnMg_nxmJlHyAak-wCcH9AU2AEtmMxgjiyDyGCQ0aKYn2nxuERWQnhJUyqFEItkibKMAWN8mZwcJdfuDZvk1nk3Dck5Nro1Nhm68cQF02JUttXGGvuUXGsfW5zWzifHzmJyhxNt_BpZqHUTcP2rr5KHs9P74cXg6ub8cnh0NRhxRtsBICJLOy2wLMpaaAmVrEoueVWhhEzovKoLWooKoCxFDVmGZY251kzEYqvksPedTMsxViO0rdeNmngz1v5DOW3U74k1z-rJvSnGBc9zGQ12vgy8e51iaNXYhBE2jbYYr1fAgManUdGh23_QFzf1Np6naColz4GDiNReT428C8FjPVsGUtWlo7p0VJ9OpLd-7j9jv-OIwG4PPBtb6Xfzj9tmD2NEsNYzOMvTzvETSaugjg</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Chen, Kuo-Yu</creator><creator>Lin, Kuen-Cherng</creator><creator>Chen, Yueh-Sheng</creator><creator>Yao, Chun-Hsu</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1654-2715</orcidid></search><sort><creationdate>20130101</creationdate><title>A Novel Porous Gelatin Composite Containing Naringin for Bone Repair</title><author>Chen, Kuo-Yu ; Lin, Kuen-Cherng ; Chen, Yueh-Sheng ; Yao, Chun-Hsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-1eee30c5327eb8bf7a91d9db595dde9147a6df82b7d11bb7f144ebfe6aa373733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acid phosphatase</topic><topic>Acid phosphatase (tartrate-resistant)</topic><topic>Acid resistance</topic><topic>Alkaline phosphatase</topic><topic>Biodegradation</topic><topic>Biomedical materials</topic><topic>Bone cancer</topic><topic>Bone grafts</topic><topic>Bone healing</topic><topic>Calcium phosphates</topic><topic>Chinese medicine</topic><topic>Citrus fruits</topic><topic>Colorimetry</topic><topic>Diabetes</topic><topic>Flavonoids</topic><topic>Gelatin</topic><topic>Genipin</topic><topic>Grafting</topic><topic>Growth factors</topic><topic>Implantation</topic><topic>Laboratory animals</topic><topic>Leaching</topic><topic>Medical treatment</topic><topic>Mitochondria</topic><topic>Osteoblasts</topic><topic>Osteoporosis</topic><topic>Phosphatase</topic><topic>R&D</topic><topic>Research & development</topic><topic>Substitute bone</topic><topic>Tricalcium phosphate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Kuo-Yu</creatorcontrib><creatorcontrib>Lin, Kuen-Cherng</creatorcontrib><creatorcontrib>Chen, Yueh-Sheng</creatorcontrib><creatorcontrib>Yao, Chun-Hsu</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Kuo-Yu</au><au>Lin, Kuen-Cherng</au><au>Chen, Yueh-Sheng</au><au>Yao, Chun-Hsu</au><au>Wang, Chong-Zhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Novel Porous Gelatin Composite Containing Naringin for Bone Repair</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>2013</volume><issue>2013</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>As Gu-Sui-Bu (GSB) is a commonly used Chinese medical herb for therapeutic treatment of bone-related diseases, naringin is its main active component. This study elucidates how various concentrations of naringin solution affect the activities of bone cells, based on colorimetric, alkaline phosphatase activity, nodule formation, and tartrate-resistant acid phosphatase activity assays to determine the optimal concentration of naringin. GGT composite was obtained by combining genipin cross-linked gelatin and β-tricalcium phosphate. GGTN composite was prepared by mixing GGT composite with the predetermined concentration of naringin. Porous GGT and GGTN composites were then made using a salt-leaching procedure. The potential of the composites in repairing bone defects was evaluated and compared in vivo by using the biological response of rabbit calvarial bone to these composites. Consequently, the most effective concentration of naringin was 10 mg/mL, which significantly enhanced the proliferation of osteoblasts, osteoclast activity, and nodule formation without affecting the alkaline phosphatase activity of osteoblasts and mitochondrial activity of mixed-bone cells. Radiographic analysis revealed greater new bone ingrowth in the GGTN composite than in the GGT composite at the same implantation time. Therefore, the GGTN composite is highly promising for use as a bone graft material.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>23431335</pmid><doi>10.1155/2013/283941</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-1654-2715</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acid phosphatase Acid phosphatase (tartrate-resistant) Acid resistance Alkaline phosphatase Biodegradation Biomedical materials Bone cancer Bone grafts Bone healing Calcium phosphates Chinese medicine Citrus fruits Colorimetry Diabetes Flavonoids Gelatin Genipin Grafting Growth factors Implantation Laboratory animals Leaching Medical treatment Mitochondria Osteoblasts Osteoporosis Phosphatase R&D Research & development Substitute bone Tricalcium phosphate |
| title | A Novel Porous Gelatin Composite Containing Naringin for Bone Repair |
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