Targeted vertical cross-sectional imaging with handheld near-infrared dual axes confocal fluorescence endomicroscope

Targeted vertical cross-sectional imaging with handheld near-infrared dual axes confocal fluorescence endomicroscope

We demonstrate vertical cross-sectional (XZ-plane) images of near-infrared (NIR) fluorescence with a handheld dual axes confocal endomicroscope that reveals specific binding of a Cy5.5-labeled peptide to pre-malignant colonic mucosa. This view is perpendicular to the tissue surface, and is similar t...

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Veröffentlicht in:Biomedical optics express 2013-02, Vol.4 (2), p.322-330
Hauptverfasser: Qiu, Zhen, Liu, Zhongyao, Duan, Xiyu, Khondee, Supang, Joshi, Bishnu, Mandella, Michael J, Oldham, Kenn, Kurabayashi, Katsuo, Wang, Thomas D
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container_end_page 330
container_issue 2
container_start_page 322
container_title Biomedical optics express
container_volume 4
creator Qiu, Zhen
Liu, Zhongyao
Duan, Xiyu
Khondee, Supang
Joshi, Bishnu
Mandella, Michael J
Oldham, Kenn
Kurabayashi, Katsuo
Wang, Thomas D
description We demonstrate vertical cross-sectional (XZ-plane) images of near-infrared (NIR) fluorescence with a handheld dual axes confocal endomicroscope that reveals specific binding of a Cy5.5-labeled peptide to pre-malignant colonic mucosa. This view is perpendicular to the tissue surface, and is similar to that used by pathologists. The scan head is 10 mm in outer diameter (OD), and integrates a one dimensional (1-D) microelectromechanical systems (MEMS) X-axis scanner and a bulky lead zirconate titanate (PZT) based Z-axis actuator. The microscope images in a raster-scanning pattern with a ±6 degrees (mechanical) scan angle at ~3 kHz in the X-axis (fast) and up to 10 Hz (0-400 μm) in the Z-axis (slow). Vertical cross-sectional fluorescence images are collected with a transverse and axial resolution of 4 and 5 μm, respectively, over a field-of-view of 800 μm (width) × 400 μm (depth). NIR vertical cross-sectional fluorescence images of fresh mouse colonic mucosa demonstrate histology-like imaging performance with this miniature instrument.
doi_str_mv 10.1364/BOE.4.000322
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title Targeted vertical cross-sectional imaging with handheld near-infrared dual axes confocal fluorescence endomicroscope
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