Spectral Flow Cytometry
Interest in measuring the complete fluorescence spectra of individual cells in flow can be traced to the earliest days of flow cytometry. Recent advances in detectors, optics, and computation have made it possible to make full spectral measurements in the sub‐millisecond time frame in which flow cyt...
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| Veröffentlicht in: | Current Protocols in Cytometry 2013-01, Vol.63 (1), p.1.27.1-1.27.13 |
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| container_end_page | 1.27.13 |
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| container_title | Current Protocols in Cytometry |
| container_volume | 63 |
| creator | Nolan, John P. Condello, Danilo |
| description | Interest in measuring the complete fluorescence spectra of individual cells in flow can be traced to the earliest days of flow cytometry. Recent advances in detectors, optics, and computation have made it possible to make full spectral measurements in the sub‐millisecond time frame in which flow cytometry measurements typically occur. This opens up new possibilities for applying spectroscopy to the analysis of individual cells. This unit reviews historical and contemporary approaches to spectral flow cytometry, as well as instrument design, calibration, and data analysis for spectral flow cytometry applications. Curr. Protoc. Cytom. 63:1.27.1‐1.27.13. © 2013 by John Wiley & Sons, Inc. |
| doi_str_mv | 10.1002/0471142956.cy0127s63 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1934-9297 |
| ispartof | Current Protocols in Cytometry, 2013-01, Vol.63 (1), p.1.27.1-1.27.13 |
| issn | 1934-9297 1934-9300 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3556726 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Calibration CCD Flow Cytometry - history Flow Cytometry - instrumentation Flow Cytometry - methods Flow Cytometry - standards fluorescence History, 20th Century History, 21st Century Humans Models, Biological SERS Single-Cell Analysis - instrumentation Single-Cell Analysis - methods spectral flow cytometry spectrograph Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods Statistics as Topic - instrumentation Statistics as Topic - methods surface enhanced Raman scattering |
| title | Spectral Flow Cytometry |
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