Spectroscopic and Functional Characterization of Iron–Sulfur Cluster-Bound Forms of Azotobacter vinelandii NifIscA

The mechanism of [4Fe-4S] cluster assembly on A-type Fe–S cluster assembly proteins, in general, and the specific role of NifIscA in the maturation of nitrogen fixation proteins are currently unknown. To address these questions, in vitro spectroscopic studies (UV–visible absorption/CD, resonance Ram...

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Veröffentlicht in:Biochemistry (Easton) 2012-10, Vol.51 (41), p.8071-8084
Hauptverfasser: Mapolelo, Daphne T, Zhang, Bo, Naik, Sunil G, Huynh, Boi Hanh, Johnson, Michael K
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container_end_page 8084
container_issue 41
container_start_page 8071
container_title Biochemistry (Easton)
container_volume 51
creator Mapolelo, Daphne T
Zhang, Bo
Naik, Sunil G
Huynh, Boi Hanh
Johnson, Michael K
description The mechanism of [4Fe-4S] cluster assembly on A-type Fe–S cluster assembly proteins, in general, and the specific role of NifIscA in the maturation of nitrogen fixation proteins are currently unknown. To address these questions, in vitro spectroscopic studies (UV–visible absorption/CD, resonance Raman and Mössbauer) have been used to investigate the mechanism of [4Fe-4S] cluster assembly on Azotobacter vinelandii NifIscA, and the ability of NifIscA to accept clusters from NifU and to donate clusters to the apo form of the nitrogenase Fe-protein. The results show that NifIscA can rapidly and reversibly cycle between forms containing one [2Fe-2S]2+ and one [4Fe-4S]2+ cluster per homodimer via DTT-induced two-electron reductive coupling of two [2Fe-2S]2+ clusters and O2-induced [4Fe-4S]2+ oxidative cleavage. This unique type of cluster interconversion in response to cellular redox status and oxygen levels is likely to be important for the specific role of A-type proteins in the maturation of [4Fe-4S] cluster-containing proteins under aerobic growth or oxidative stress conditions. Only the [4Fe-4S]2+-NifIscA was competent for rapid activation of apo-nitrogenase Fe protein under anaerobic conditions. Apo-NifIscA was shown to accept clusters from [4Fe-4S] cluster-bound NifU via rapid intact cluster transfer, indicating a potential role as a cluster carrier for delivery of clusters assembled on NifU. Overall the results support the proposal that A-type proteins can function as carrier proteins for clusters assembled on U-type proteins and suggest that they are likely to supply [2Fe-2S] clusters rather than [4Fe-4S] for the maturation of [4Fe-4S] cluster-containing proteins under aerobic or oxidative stress growth conditions.
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title Spectroscopic and Functional Characterization of Iron–Sulfur Cluster-Bound Forms of Azotobacter vinelandii NifIscA
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