Prevalence of HIV and HCV infections in two populations of Malian women and serological assays performances

AIM:To estimate the prevalence of human immunodeficiency virus(HIV) and hepatitis C virus(HCV) infections in women in Mali and to evaluate the performance of serological assays.METHODS:Two prospective studies were conducted in 2009 and 2010 in Mali.They concerned first,1000 pregnant women attending six reference health centers in Bamako(Malian capital) between May 26 and June 16,2009;and secondly,231 women over 50 years who consulted general practitioners of two hospitals in Bamako between October 25 and December 24,2010.Blood samples were collected and kept frozen in good condition before analysis.All samples depicted as positive using HIV/HCV enzyme immuno-assay screening assays were submitted to confirmation analysis.Molecular markers of HCV were characterized.RESULTS:The seroprevalence of HIV and HCV in the population of pregnant women was 4.1% and 0.2% respectively.Among older women the seroprevalence was higher and similar for HIV and HCV(6.1% vs 6.5%).The anti-HIV prevalence was not different in young and older women(4.1% vs 6.1%).In contrast,the anti-HCV prevalence was higher in older compared to younger women(6.5% vs 0.2%,P < 0.01).Of 2 pregnant women who were HCV seropositive,only one was polymerase chain reaction(PCR) reactive and infected by genotype 2,with a viral load of 1600 IU/mL.Regarding older women who were HCV seropositive,13 out of 15 were PCR reactive,infected by genotype 1 or 2.Globally HCV genotype 2 was predominant.The positive predictive value(PPV) measured with VIKIA HIV test in young women was 100% therefore significantly higher than the 87.5% measured in older women(P < 0.05).Conversely,the PPV measured with Monolisa HCV assay in older women was 88.2% and higher than the 14.3% measured in younger women(P < 0.01).CONCLUSION:Whereas HIV prevalence was similar in both subpopulations HCV was more frequent among older women(P < 0.01).The PPV of screening assays varied with the age of the subjects.