Alternative lengthening of telomeres in normal mammalian somatic cells
Some cancers use alternative lengthening of telomeres (ALT), a mechanism whereby new telomeric DNA is synthesized from a DNA template. To determine whether normal mammalian tissues have ALT activity, we generated a mouse strain containing a DNA tag in a single telomere. We found that the tagged telo...
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| Veröffentlicht in: | Genes & development 2013-01, Vol.27 (1), p.18-23 |
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| container_title | Genes & development |
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| creator | Neumann, Axel A Watson, Catherine M Noble, Jane R Pickett, Hilda A Tam, Patrick P L Reddel, Roger R |
| description | Some cancers use alternative lengthening of telomeres (ALT), a mechanism whereby new telomeric DNA is synthesized from a DNA template. To determine whether normal mammalian tissues have ALT activity, we generated a mouse strain containing a DNA tag in a single telomere. We found that the tagged telomere was copied by other telomeres in somatic tissues but not the germline. The tagged telomere was also copied by other telomeres when introgressed into CAST/EiJ mice, which have telomeres more similar in length to those of humans. We conclude that ALT activity occurs in normal mouse somatic tissues. |
| doi_str_mv | 10.1101/gad.205062.112 |
| format | Article |
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To determine whether normal mammalian tissues have ALT activity, we generated a mouse strain containing a DNA tag in a single telomere. We found that the tagged telomere was copied by other telomeres in somatic tissues but not the germline. The tagged telomere was also copied by other telomeres when introgressed into CAST/EiJ mice, which have telomeres more similar in length to those of humans. We conclude that ALT activity occurs in normal mouse somatic tissues.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>23307865</pmid><doi>10.1101/gad.205062.112</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals B-Lymphocytes - cytology Breeding Cell Line Chimera - genetics Chromosomes - genetics Chromosomes - metabolism Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Female Genotyping Techniques Keratinocytes - cytology Keratinocytes - metabolism Keratinocytes - physiology Male Mammals Mice Mice, Inbred C57BL Research Communication Spermatocytes - cytology Spermatocytes - physiology Staining and Labeling T-Lymphocytes - cytology Telomere Homeostasis - genetics |
| title | Alternative lengthening of telomeres in normal mammalian somatic cells |
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