Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions
We report constitutive production of glucose isomerase (GI) under submerged growth of Aspergillus sp. in glucose phosphate broth (GPB). The fungus produced significant quantities of extracellular GI in GPB without supplementing the inducer (xylose). The maximum biomass (872 mg) and highest level of...
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| Veröffentlicht in: | Journal of food science and technology 2010-10, Vol.47 (5), p.496-500 |
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| creator | Sayyed, Riyaz Zafar Shimpi, G. B. Chincholkar, S. B. |
| description | We report constitutive production of glucose isomerase (GI) under submerged growth of
Aspergillus
sp. in glucose phosphate broth (GPB). The fungus produced significant quantities of extracellular GI in GPB without supplementing the inducer (xylose). The maximum biomass (872 mg) and highest level of GI (1126 U) were obtained in 42 h at 30 °C and 120 rpm. Equal level of biomass and enzyme were produced in GPB with glucose and xylose, but the amount of biomass and enzyme was drastically reduced when the fungus was grown on other carbon sources. Optimum biomass, enzyme units and enzyme activity were obtained with 40 and 1 g/l of glucose, respectively. Growth of
Aspergillus
sp. and enzyme synthesis even at high glucose concentration (60 g/l) indicated the osmophillic nature of the fungus. Increasing the glucose concentration above 1 and 40 g/l did not support the growth and enzyme activity. Among various organic and inorganic nitrogen sources used, yeast extract, peptone and NH
4
SO
4
gave the best biomass and enzyme yields. Addition of Mg
2+
and Mn
2+
in GPB significantly enhanced the enzyme production. Under optimized conditions in modified GPB, the yield of biomass and synthesis and activity of GI were significantly enhanced. |
| doi_str_mv | 10.1007/s13197-010-0084-3 |
| format | Article |
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Aspergillus
sp. in glucose phosphate broth (GPB). The fungus produced significant quantities of extracellular GI in GPB without supplementing the inducer (xylose). The maximum biomass (872 mg) and highest level of GI (1126 U) were obtained in 42 h at 30 °C and 120 rpm. Equal level of biomass and enzyme were produced in GPB with glucose and xylose, but the amount of biomass and enzyme was drastically reduced when the fungus was grown on other carbon sources. Optimum biomass, enzyme units and enzyme activity were obtained with 40 and 1 g/l of glucose, respectively. Growth of
Aspergillus
sp. and enzyme synthesis even at high glucose concentration (60 g/l) indicated the osmophillic nature of the fungus. Increasing the glucose concentration above 1 and 40 g/l did not support the growth and enzyme activity. Among various organic and inorganic nitrogen sources used, yeast extract, peptone and NH
4
SO
4
gave the best biomass and enzyme yields. Addition of Mg
2+
and Mn
2+
in GPB significantly enhanced the enzyme production. Under optimized conditions in modified GPB, the yield of biomass and synthesis and activity of GI were significantly enhanced.</description><identifier>ISSN: 0022-1155</identifier><identifier>EISSN: 0975-8402</identifier><identifier>DOI: 10.1007/s13197-010-0084-3</identifier><identifier>PMID: 23572677</identifier><language>eng</language><publisher>India: Springer-Verlag</publisher><subject>Aspergillus ; Biomass ; Carbon ; Carbon sources ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Corn syrup ; Enzymatic activity ; Enzymes ; Food ; Food Science ; Fungi ; Glucose ; Nitrogen ; Nitrogen sources ; Nutrition ; Original ; Original Article ; Peptones ; Reagents ; Yeast ; Yeasts</subject><ispartof>Journal of food science and technology, 2010-10, Vol.47 (5), p.496-500</ispartof><rights>Association of Food Scientists & Technologists (India) 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-e247009e313887ba8b43a1dfbfc79145612bba6253ed22ac0f401f2e346677e73</citedby><cites>FETCH-LOGICAL-c501t-e247009e313887ba8b43a1dfbfc79145612bba6253ed22ac0f401f2e346677e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551096/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551096/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23572677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sayyed, Riyaz Zafar</creatorcontrib><creatorcontrib>Shimpi, G. B.</creatorcontrib><creatorcontrib>Chincholkar, S. B.</creatorcontrib><title>Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions</title><title>Journal of food science and technology</title><addtitle>J Food Sci Technol</addtitle><addtitle>J Food Sci Technol</addtitle><description>We report constitutive production of glucose isomerase (GI) under submerged growth of
Aspergillus
sp. in glucose phosphate broth (GPB). The fungus produced significant quantities of extracellular GI in GPB without supplementing the inducer (xylose). The maximum biomass (872 mg) and highest level of GI (1126 U) were obtained in 42 h at 30 °C and 120 rpm. Equal level of biomass and enzyme were produced in GPB with glucose and xylose, but the amount of biomass and enzyme was drastically reduced when the fungus was grown on other carbon sources. Optimum biomass, enzyme units and enzyme activity were obtained with 40 and 1 g/l of glucose, respectively. Growth of
Aspergillus
sp. and enzyme synthesis even at high glucose concentration (60 g/l) indicated the osmophillic nature of the fungus. Increasing the glucose concentration above 1 and 40 g/l did not support the growth and enzyme activity. Among various organic and inorganic nitrogen sources used, yeast extract, peptone and NH
4
SO
4
gave the best biomass and enzyme yields. Addition of Mg
2+
and Mn
2+
in GPB significantly enhanced the enzyme production. Under optimized conditions in modified GPB, the yield of biomass and synthesis and activity of GI were significantly enhanced.</description><subject>Aspergillus</subject><subject>Biomass</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Corn syrup</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Food</subject><subject>Food Science</subject><subject>Fungi</subject><subject>Glucose</subject><subject>Nitrogen</subject><subject>Nitrogen sources</subject><subject>Nutrition</subject><subject>Original</subject><subject>Original Article</subject><subject>Peptones</subject><subject>Reagents</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0022-1155</issn><issn>0975-8402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kstu1TAQhiMEolXpA7BBFhvYpIxvcbJBqo5oQarEBtaW4zipqxw7eOKWvj2OTikXCbzxSPPN75nxX1UvKZxRAPUOKaedqoFCDdCKmj-pjqFTsm4FsKclBsZqSqU8qk4Rb6AczlTL4Hl1xLhUrFHquLrbxYCrX_Pqbx1ZUhyyXX0MJI7EfV-TsW6e82wSmeZsIzriMe5dMiXq74kpIO7jcu3n2VtyjotLU4kzElzOSA6DSwRzXyomNxAbw-A3eXxRPRvNjO704T6pvl58-LL7WF99vvy0O7-qrQS61o4JBdA5Tnnbqt60veCGDmM_WtVRIRvK-t40THI3MGYsjALoyBwXTRnPKX5SvT_oLlsTg3WhzDTrJfm9Sfc6Gq__zAR_rad4q7mUFLqmCLx5EEjxW3a46r3HbSkmuJhRd6CoEo3khXz7X5Jy1lDBOd9EX_-F3sScQlmE7grUQidYgegBsikiJjc-dk1BbxbQBwvoYgG9WUBvPbz6fdzHip8fXgB2ALCkwuTSr5f_rfoDSam-Zg</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Sayyed, Riyaz Zafar</creator><creator>Shimpi, G. B.</creator><creator>Chincholkar, S. B.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>04Q</scope><scope>04S</scope><scope>04W</scope><scope>0U~</scope><scope>1-H</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7RQ</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X2</scope><scope>7XB</scope><scope>87Z</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>F~G</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>L.-</scope><scope>L.0</scope><scope>L6V</scope><scope>M0C</scope><scope>M0K</scope><scope>M7S</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>SOI</scope><scope>7X8</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20101001</creationdate><title>Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions</title><author>Sayyed, Riyaz Zafar ; Shimpi, G. B. ; Chincholkar, S. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-e247009e313887ba8b43a1dfbfc79145612bba6253ed22ac0f401f2e346677e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aspergillus</topic><topic>Biomass</topic><topic>Carbon</topic><topic>Carbon sources</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Corn syrup</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Food</topic><topic>Food Science</topic><topic>Fungi</topic><topic>Glucose</topic><topic>Nitrogen</topic><topic>Nitrogen sources</topic><topic>Nutrition</topic><topic>Original</topic><topic>Original Article</topic><topic>Peptones</topic><topic>Reagents</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sayyed, Riyaz Zafar</creatorcontrib><creatorcontrib>Shimpi, G. 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B.</au><au>Chincholkar, S. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions</atitle><jtitle>Journal of food science and technology</jtitle><stitle>J Food Sci Technol</stitle><addtitle>J Food Sci Technol</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>47</volume><issue>5</issue><spage>496</spage><epage>500</epage><pages>496-500</pages><issn>0022-1155</issn><eissn>0975-8402</eissn><abstract>We report constitutive production of glucose isomerase (GI) under submerged growth of
Aspergillus
sp. in glucose phosphate broth (GPB). The fungus produced significant quantities of extracellular GI in GPB without supplementing the inducer (xylose). The maximum biomass (872 mg) and highest level of GI (1126 U) were obtained in 42 h at 30 °C and 120 rpm. Equal level of biomass and enzyme were produced in GPB with glucose and xylose, but the amount of biomass and enzyme was drastically reduced when the fungus was grown on other carbon sources. Optimum biomass, enzyme units and enzyme activity were obtained with 40 and 1 g/l of glucose, respectively. Growth of
Aspergillus
sp. and enzyme synthesis even at high glucose concentration (60 g/l) indicated the osmophillic nature of the fungus. Increasing the glucose concentration above 1 and 40 g/l did not support the growth and enzyme activity. Among various organic and inorganic nitrogen sources used, yeast extract, peptone and NH
4
SO
4
gave the best biomass and enzyme yields. Addition of Mg
2+
and Mn
2+
in GPB significantly enhanced the enzyme production. Under optimized conditions in modified GPB, the yield of biomass and synthesis and activity of GI were significantly enhanced.</abstract><cop>India</cop><pub>Springer-Verlag</pub><pmid>23572677</pmid><doi>10.1007/s13197-010-0084-3</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of food science and technology, 2010-10, Vol.47 (5), p.496-500 |
| issn | 0022-1155 0975-8402 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3551096 |
| source | SpringerNature Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Aspergillus Biomass Carbon Carbon sources Chemistry Chemistry and Materials Science Chemistry/Food Science Corn syrup Enzymatic activity Enzymes Food Food Science Fungi Glucose Nitrogen Nitrogen sources Nutrition Original Original Article Peptones Reagents Yeast Yeasts |
| title | Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions |
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