Rabies in South Indian Cows: An evidence of Sri Lankan Rabies virus Variant Infection Based on the Analysis of Partial Nucleoprotein Gene
Rabies is a highly fatal non-suppurative encephalomyelitis, caused by the Rabies virus . Dogs are the major reservoir of rabies in India and are the source of infection to other domestic animals. In this report, laboratory investigation and molecular characterization of isolates from two cows with p...
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| Veröffentlicht in: | Indian Journal of Virology 2011-12, Vol.22 (2), p.138-141, Article 138 |
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| creator | AravindhBabu, R. P. Manoharan, S. Ramadass, P. Chandran, N. D. J. |
| description | Rabies is a highly fatal non-suppurative encephalomyelitis, caused by the
Rabies virus
. Dogs are the major reservoir of rabies in India and are the source of infection to other domestic animals. In this report, laboratory investigation and molecular characterization of isolates from two cows with paralytic rabies is described. Necropsy brain samples from the two cows were tested for the presence of rabies antigen using a fluorescent antibody test and the results were confirmed using RT-PCR.
Rabies virus
was successfully isolated from both the brain samples in a murine neuroblastoma cell line. The phylogenetic analysis of partial nucleoprotein gene sequences of these isolates showed them to be of a variant of
Rabies virus
which is closely related to the Sri Lankan
Rabies virus
lineage as previously reported. In addition, partial nucleoprotein genes of 19 more
Rabies virus
isolates from southern India were sequenced and of these 11 isolates were found to be closely related to the Sri Lankan lineage. The deduced amino acid sequences of the partial nucleoprotein of the Indian isolates were 96–99% identical to the Sri Lankan isolates. This investigation re-confirms the previous speculations that the Sri Lankan variant of the virus may still be actively transmitted by animals in India. |
| doi_str_mv | 10.1007/s13337-011-0050-3 |
| format | Article |
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Rabies virus
. Dogs are the major reservoir of rabies in India and are the source of infection to other domestic animals. In this report, laboratory investigation and molecular characterization of isolates from two cows with paralytic rabies is described. Necropsy brain samples from the two cows were tested for the presence of rabies antigen using a fluorescent antibody test and the results were confirmed using RT-PCR.
Rabies virus
was successfully isolated from both the brain samples in a murine neuroblastoma cell line. The phylogenetic analysis of partial nucleoprotein gene sequences of these isolates showed them to be of a variant of
Rabies virus
which is closely related to the Sri Lankan
Rabies virus
lineage as previously reported. In addition, partial nucleoprotein genes of 19 more
Rabies virus
isolates from southern India were sequenced and of these 11 isolates were found to be closely related to the Sri Lankan lineage. The deduced amino acid sequences of the partial nucleoprotein of the Indian isolates were 96–99% identical to the Sri Lankan isolates. This investigation re-confirms the previous speculations that the Sri Lankan variant of the virus may still be actively transmitted by animals in India.</description><identifier>ISSN: 0970-2822</identifier><identifier>ISSN: 2347-3584</identifier><identifier>EISSN: 0974-0120</identifier><identifier>EISSN: 2347-3517</identifier><identifier>DOI: 10.1007/s13337-011-0050-3</identifier><identifier>PMID: 23637516</identifier><language>eng</language><publisher>India: Springer-Verlag</publisher><subject>Analysis ; Antibodies ; Antigens ; Bats ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; Cattle ; Cell Biology ; Disease transmission ; Domestic animals ; Encephalomyelitis ; Epidemiology ; Fluorescent antibody test ; Genes ; Genomes ; Infection ; Life Sciences ; Lyssavirus ; Microbiology ; Necropsy ; Neuroblastoma ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Protein Structure ; Rabies ; Short Communication ; Viruses</subject><ispartof>Indian Journal of Virology, 2011-12, Vol.22 (2), p.138-141, Article 138</ispartof><rights>Indian Virological Society 2011</rights><rights>COPYRIGHT 2011 Springer</rights><rights>Indian Virological Society 2011.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-286e7378ae700dd88efc077365fbbf3d8bc95b5f671657d86f8e6397324735213</citedby><cites>FETCH-LOGICAL-c537t-286e7378ae700dd88efc077365fbbf3d8bc95b5f671657d86f8e6397324735213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3550735/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2920284803?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21388,21389,27924,27925,33530,33744,43659,43805,53791,53793,64385,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23637516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AravindhBabu, R. P.</creatorcontrib><creatorcontrib>Manoharan, S.</creatorcontrib><creatorcontrib>Ramadass, P.</creatorcontrib><creatorcontrib>Chandran, N. D. J.</creatorcontrib><title>Rabies in South Indian Cows: An evidence of Sri Lankan Rabies virus Variant Infection Based on the Analysis of Partial Nucleoprotein Gene</title><title>Indian Journal of Virology</title><addtitle>Indian J. Virol</addtitle><addtitle>Indian J Virol</addtitle><description>Rabies is a highly fatal non-suppurative encephalomyelitis, caused by the
Rabies virus
. Dogs are the major reservoir of rabies in India and are the source of infection to other domestic animals. In this report, laboratory investigation and molecular characterization of isolates from two cows with paralytic rabies is described. Necropsy brain samples from the two cows were tested for the presence of rabies antigen using a fluorescent antibody test and the results were confirmed using RT-PCR.
Rabies virus
was successfully isolated from both the brain samples in a murine neuroblastoma cell line. The phylogenetic analysis of partial nucleoprotein gene sequences of these isolates showed them to be of a variant of
Rabies virus
which is closely related to the Sri Lankan
Rabies virus
lineage as previously reported. In addition, partial nucleoprotein genes of 19 more
Rabies virus
isolates from southern India were sequenced and of these 11 isolates were found to be closely related to the Sri Lankan lineage. The deduced amino acid sequences of the partial nucleoprotein of the Indian isolates were 96–99% identical to the Sri Lankan isolates. This investigation re-confirms the previous speculations that the Sri Lankan variant of the virus may still be actively transmitted by animals in India.</description><subject>Analysis</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Bats</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Cattle</subject><subject>Cell Biology</subject><subject>Disease transmission</subject><subject>Domestic animals</subject><subject>Encephalomyelitis</subject><subject>Epidemiology</subject><subject>Fluorescent antibody test</subject><subject>Genes</subject><subject>Genomes</subject><subject>Infection</subject><subject>Life Sciences</subject><subject>Lyssavirus</subject><subject>Microbiology</subject><subject>Necropsy</subject><subject>Neuroblastoma</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Protein Structure</subject><subject>Rabies</subject><subject>Short Communication</subject><subject>Viruses</subject><issn>0970-2822</issn><issn>2347-3584</issn><issn>0974-0120</issn><issn>2347-3517</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1Ustu1DAUjRCIVqUfwAZZYsMmxY84dlggDSMolUaAKLC1HOd6xpCxp3YyqJ_Qv67TDOUhIS9s3Xse91qnKJ4SfEYwFi8TYYyJEhNSYsxxyR4Ux7gRVa5Q_PDujUsqKT0qTlNyLSa1wE2uPi6OKKuZ4KQ-Lm4-69ZBQs6jyzAOG3ThO6c9Woaf6RVaeAR714E3gIJFl9GhlfY_cv9A27s4JvRNx8wZMteCGVzw6I1O0KH8GDaQVXR_nVyaJD7pODjdow-j6SHsYhggW5-DhyfFI6v7BKeH-6T4-u7tl-X7cvXx_GK5WJWGMzHkjWoQTEgNAuOukxKswUKwmtu2tayTrWl4y20tSM1FJ2sroWaNYLQSjFPCTorXs-5ubLfQGfBD1L3aRbfV8VoF7dTfHe82ah32inGOs0QWeHEQiOFqhDSorUsG-l57CGNShFWyamTdTF7P_4F-D2PM35EUbSimspKYZdTZjFrrHpTzNmRfk08HW2eCB-tyfSFI3kiyuwnITDAxpBTB3k9PsJrCoeZwqBwONYVDTSbP_lz7nvErChlAZ0DKLb-G-HvW_6veAl_ZxCI</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>AravindhBabu, R. 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P. ; Manoharan, S. ; Ramadass, P. ; Chandran, N. D. 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P.</au><au>Manoharan, S.</au><au>Ramadass, P.</au><au>Chandran, N. D. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rabies in South Indian Cows: An evidence of Sri Lankan Rabies virus Variant Infection Based on the Analysis of Partial Nucleoprotein Gene</atitle><jtitle>Indian Journal of Virology</jtitle><stitle>Indian J. Virol</stitle><addtitle>Indian J Virol</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>22</volume><issue>2</issue><spage>138</spage><epage>141</epage><pages>138-141</pages><artnum>138</artnum><issn>0970-2822</issn><issn>2347-3584</issn><eissn>0974-0120</eissn><eissn>2347-3517</eissn><abstract>Rabies is a highly fatal non-suppurative encephalomyelitis, caused by the
Rabies virus
. Dogs are the major reservoir of rabies in India and are the source of infection to other domestic animals. In this report, laboratory investigation and molecular characterization of isolates from two cows with paralytic rabies is described. Necropsy brain samples from the two cows were tested for the presence of rabies antigen using a fluorescent antibody test and the results were confirmed using RT-PCR.
Rabies virus
was successfully isolated from both the brain samples in a murine neuroblastoma cell line. The phylogenetic analysis of partial nucleoprotein gene sequences of these isolates showed them to be of a variant of
Rabies virus
which is closely related to the Sri Lankan
Rabies virus
lineage as previously reported. In addition, partial nucleoprotein genes of 19 more
Rabies virus
isolates from southern India were sequenced and of these 11 isolates were found to be closely related to the Sri Lankan lineage. The deduced amino acid sequences of the partial nucleoprotein of the Indian isolates were 96–99% identical to the Sri Lankan isolates. This investigation re-confirms the previous speculations that the Sri Lankan variant of the virus may still be actively transmitted by animals in India.</abstract><cop>India</cop><pub>Springer-Verlag</pub><pmid>23637516</pmid><doi>10.1007/s13337-011-0050-3</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | ProQuest Central (Alumni Edition); SpringerNature Journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Analysis Antibodies Antigens Bats Biochemistry Bioinformatics Biomedical and Life Sciences Cattle Cell Biology Disease transmission Domestic animals Encephalomyelitis Epidemiology Fluorescent antibody test Genes Genomes Infection Life Sciences Lyssavirus Microbiology Necropsy Neuroblastoma Phylogenetics Phylogeny Polymerase chain reaction Protein Structure Rabies Short Communication Viruses |
| title | Rabies in South Indian Cows: An evidence of Sri Lankan Rabies virus Variant Infection Based on the Analysis of Partial Nucleoprotein Gene |
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