A Novel Adipose-Specific Gene Deletion Model Demonstrates Potential Pitfalls of Existing Methods
Adipose-specific gene deletion in mice is crucial in determining gene function in adipocyte homeostasis and the development of obesity. We noted 100% mortality when the Hdac3 gene was conditionally deleted using Fabp4-Cre mice, the most commonly used model of adipose-targeted Cre recombinase. Howeve...
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| Veröffentlicht in: | Molecular endocrinology (Baltimore, Md.) Md.), 2013-01, Vol.27 (1), p.127-134 |
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| container_title | Molecular endocrinology (Baltimore, Md.) |
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| creator | Mullican, Shannon E Tomaru, Takuya Gaddis, Christine A Peed, Lindsey C Sundaram, Anand Lazar, Mitchell A |
| description | Adipose-specific gene deletion in mice is crucial in determining gene function in adipocyte homeostasis and the development of obesity. We noted 100% mortality when the Hdac3 gene was conditionally deleted using Fabp4-Cre mice, the most commonly used model of adipose-targeted Cre recombinase. However, this surprising result was not reproduced using other models of adipose targeting of Cre, including a novel Retn-Cre mouse. These findings underscore the need for caution when interpreting data obtained using Fabp4-Cre mice and should encourage the use of additional or alternative adipose-targeting Cre mouse models before drawing conclusions about in vivo adipocyte-specific functions. |
| doi_str_mv | 10.1210/me.2012-1267 |
| format | Article |
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We noted 100% mortality when the Hdac3 gene was conditionally deleted using Fabp4-Cre mice, the most commonly used model of adipose-targeted Cre recombinase. However, this surprising result was not reproduced using other models of adipose targeting of Cre, including a novel Retn-Cre mouse. These findings underscore the need for caution when interpreting data obtained using Fabp4-Cre mice and should encourage the use of additional or alternative adipose-targeting Cre mouse models before drawing conclusions about in vivo adipocyte-specific functions.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>23192980</pmid><doi>10.1210/me.2012-1267</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular endocrinology (Baltimore, Md.), 2013-01, Vol.27 (1), p.127-134 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Journals@Ovid Complete |
| subjects | Adipose Tissue - enzymology Adipose Tissue - physiopathology Animals Disease Models, Animal Epididymis - enzymology Epididymis - physiopathology Fatty Acid-Binding Proteins - genetics Gene Deletion Genes, Lethal Genetic Engineering Histone Deacetylases - genetics Male Mice Mice, Transgenic Obesity - enzymology Obesity - genetics Organ Specificity Original Research Phenotype |
| title | A Novel Adipose-Specific Gene Deletion Model Demonstrates Potential Pitfalls of Existing Methods |
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