In Vivo and In Vitro Antinociceptive Effect of Fagopyrum cymosum (Trev.) Meisn Extracts: A Possible Action by Recovering Intestinal Barrier Dysfunction
Fagopyrum cymosum (Trev.) Meisn (Fag) is a herb rhizome which has been widely used to treat diseases. To investigate the effects and mechanisms of the Fag on irritable bowel syndrome (IBS), in vivo neonatal pups maternal separation (NMS) combined with intracolonic infusion of acetic acid (AA) was em...
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| creator | Cao, Peng Lu, Yin Shao, Ming Luo, Yi Yan, Jing Cai, Xueting Liu, Lina Sun, Zhiguang |
| description | Fagopyrum cymosum (Trev.) Meisn (Fag) is a herb rhizome which has been widely used to treat diseases. To investigate the effects and mechanisms of the Fag on irritable bowel syndrome (IBS), in vivo neonatal pups maternal separation (NMS) combined with intracolonic infusion of acetic acid (AA) was employed to establish IBS rat models. Fag reduced their visceral hyperalgesia and the whole gut permeability, ameliorated colonic mucosa inflammation and injury, and upregulated the expression of decreased tight junction proteins (TJs) of claudin-1, occludin, and ZO-1 (except ZO-2) in colonic epithelium. Caco-2 monolayer cells were incubated with TNF-α and IFN-γ in vitro to establish an epithelial barrier dysfunction model whose transepithelial electrical resistance (TER) depended more on dose of Fag than that of the controls, and whose TJs levels were lower than those of the controls. Fag upregulated the NP-40 insoluble and soluble components of the four TJs markedly in a dose-dependent manner. These data suggest that Fag alleviated the hyperalgesia of IBS rats by reducing intestinal inflammation and enhancing mucosal epithelial function after regulating the structure and function of TJs. |
| doi_str_mv | 10.1155/2012/983801 |
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Meisn Extracts: A Possible Action by Recovering Intestinal Barrier Dysfunction</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Cao, Peng ; Lu, Yin ; Shao, Ming ; Luo, Yi ; Yan, Jing ; Cai, Xueting ; Liu, Lina ; Sun, Zhiguang</creator><contributor>Shirwaikar, Annie ; Annie Shirwaikar</contributor><creatorcontrib>Cao, Peng ; Lu, Yin ; Shao, Ming ; Luo, Yi ; Yan, Jing ; Cai, Xueting ; Liu, Lina ; Sun, Zhiguang ; Shirwaikar, Annie ; Annie Shirwaikar</creatorcontrib><description>Fagopyrum cymosum (Trev.) Meisn (Fag) is a herb rhizome which has been widely used to treat diseases. To investigate the effects and mechanisms of the Fag on irritable bowel syndrome (IBS), in vivo neonatal pups maternal separation (NMS) combined with intracolonic infusion of acetic acid (AA) was employed to establish IBS rat models. Fag reduced their visceral hyperalgesia and the whole gut permeability, ameliorated colonic mucosa inflammation and injury, and upregulated the expression of decreased tight junction proteins (TJs) of claudin-1, occludin, and ZO-1 (except ZO-2) in colonic epithelium. Caco-2 monolayer cells were incubated with TNF-α and IFN-γ in vitro to establish an epithelial barrier dysfunction model whose transepithelial electrical resistance (TER) depended more on dose of Fag than that of the controls, and whose TJs levels were lower than those of the controls. Fag upregulated the NP-40 insoluble and soluble components of the four TJs markedly in a dose-dependent manner. These data suggest that Fag alleviated the hyperalgesia of IBS rats by reducing intestinal inflammation and enhancing mucosal epithelial function after regulating the structure and function of TJs.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2012/983801</identifier><identifier>PMID: 23365604</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Abdomen ; Acetic acid ; Animal models ; Colon ; Epithelium ; Fagopyrum ; Hyperalgesia ; Intestine ; Irritable bowel syndrome ; Kinases ; Mucosa ; Neonates ; Pain perception ; Permeability ; Proteins ; Rodents ; Small intestine ; Structure-function relationships ; Tumor necrosis factor-α ; Zonula occludens-1 protein ; γ-Interferon</subject><ispartof>Evidence-based complementary and alternative medicine, 2012-01, Vol.2012 (2012), p.1-13</ispartof><rights>Copyright © 2012 Lina Liu et al.</rights><rights>Copyright © 2012 Lina Liu et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2012 Lina Liu et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-f186b658e98d852e961fb9db500dc330ae90480e26322a673a2141f0fcd438683</citedby><cites>FETCH-LOGICAL-c430t-f186b658e98d852e961fb9db500dc330ae90480e26322a673a2141f0fcd438683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541707/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541707/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23365604$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Shirwaikar, Annie</contributor><contributor>Annie Shirwaikar</contributor><creatorcontrib>Cao, Peng</creatorcontrib><creatorcontrib>Lu, Yin</creatorcontrib><creatorcontrib>Shao, Ming</creatorcontrib><creatorcontrib>Luo, Yi</creatorcontrib><creatorcontrib>Yan, Jing</creatorcontrib><creatorcontrib>Cai, Xueting</creatorcontrib><creatorcontrib>Liu, Lina</creatorcontrib><creatorcontrib>Sun, Zhiguang</creatorcontrib><title>In Vivo and In Vitro Antinociceptive Effect of Fagopyrum cymosum (Trev.) Meisn Extracts: A Possible Action by Recovering Intestinal Barrier Dysfunction</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Fagopyrum cymosum (Trev.) Meisn (Fag) is a herb rhizome which has been widely used to treat diseases. To investigate the effects and mechanisms of the Fag on irritable bowel syndrome (IBS), in vivo neonatal pups maternal separation (NMS) combined with intracolonic infusion of acetic acid (AA) was employed to establish IBS rat models. Fag reduced their visceral hyperalgesia and the whole gut permeability, ameliorated colonic mucosa inflammation and injury, and upregulated the expression of decreased tight junction proteins (TJs) of claudin-1, occludin, and ZO-1 (except ZO-2) in colonic epithelium. Caco-2 monolayer cells were incubated with TNF-α and IFN-γ in vitro to establish an epithelial barrier dysfunction model whose transepithelial electrical resistance (TER) depended more on dose of Fag than that of the controls, and whose TJs levels were lower than those of the controls. Fag upregulated the NP-40 insoluble and soluble components of the four TJs markedly in a dose-dependent manner. These data suggest that Fag alleviated the hyperalgesia of IBS rats by reducing intestinal inflammation and enhancing mucosal epithelial function after regulating the structure and function of TJs.</description><subject>Abdomen</subject><subject>Acetic acid</subject><subject>Animal models</subject><subject>Colon</subject><subject>Epithelium</subject><subject>Fagopyrum</subject><subject>Hyperalgesia</subject><subject>Intestine</subject><subject>Irritable bowel syndrome</subject><subject>Kinases</subject><subject>Mucosa</subject><subject>Neonates</subject><subject>Pain perception</subject><subject>Permeability</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Small intestine</subject><subject>Structure-function relationships</subject><subject>Tumor necrosis factor-α</subject><subject>Zonula occludens-1 protein</subject><subject>γ-Interferon</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkU9v1DAQxSMEoqVw4o4scSlU2_pPYjs9IC1lC5WKQKggbpbjjLeuEnuxk8B-Er5u3W5ZFU6cZqT56c2beUXxnOBDQqrqiGJCj2rJJCYPil0iSjIrqZQPt734vlM8SekKY1oLIR4XO5QxXnFc7ha_zzz65qaAtG_RbT_EgOZ-cD4YZ2A1uAnQwlowAwoWneplWK3j2COz7kPKdf8iwnT4Cn0Elzxa_BqiNkM6RnP0OaTkmg7Q3AwueNSs0RcwYYLo_DIvGyDlNbpDb3WMDiJ6t0529Lfw0-KR1V2CZ3d1r_h6urg4-TA7__T-7GR-PjMlw8PMEskbXkmoZSsrCjUntqnbpsK4NYxhDTUuJQbKGaWaC6YpKYnF1rQlk1yyveLNRnc1Nj20Bnz236lVdL2OaxW0U39PvLtUyzApVpVEYJEF9u8EYvgx5otU75KBrtMewpgUoZIJKjDjGX35D3oVxpgfkBTFHEssJa0ydbChTMz_i2C3ZghWN4Grm8DVJvBMv7jvf8v-STgDrzfApfOt_un-Tw0yAlbfg6nk-Yhrz229XA</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Cao, Peng</creator><creator>Lu, Yin</creator><creator>Shao, Ming</creator><creator>Luo, Yi</creator><creator>Yan, Jing</creator><creator>Cai, Xueting</creator><creator>Liu, Lina</creator><creator>Sun, Zhiguang</creator><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>In Vivo and In Vitro Antinociceptive Effect of Fagopyrum cymosum (Trev.) 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Meisn Extracts: A Possible Action by Recovering Intestinal Barrier Dysfunction</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Fagopyrum cymosum (Trev.) Meisn (Fag) is a herb rhizome which has been widely used to treat diseases. To investigate the effects and mechanisms of the Fag on irritable bowel syndrome (IBS), in vivo neonatal pups maternal separation (NMS) combined with intracolonic infusion of acetic acid (AA) was employed to establish IBS rat models. Fag reduced their visceral hyperalgesia and the whole gut permeability, ameliorated colonic mucosa inflammation and injury, and upregulated the expression of decreased tight junction proteins (TJs) of claudin-1, occludin, and ZO-1 (except ZO-2) in colonic epithelium. Caco-2 monolayer cells were incubated with TNF-α and IFN-γ in vitro to establish an epithelial barrier dysfunction model whose transepithelial electrical resistance (TER) depended more on dose of Fag than that of the controls, and whose TJs levels were lower than those of the controls. Fag upregulated the NP-40 insoluble and soluble components of the four TJs markedly in a dose-dependent manner. These data suggest that Fag alleviated the hyperalgesia of IBS rats by reducing intestinal inflammation and enhancing mucosal epithelial function after regulating the structure and function of TJs.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>23365604</pmid><doi>10.1155/2012/983801</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abdomen Acetic acid Animal models Colon Epithelium Fagopyrum Hyperalgesia Intestine Irritable bowel syndrome Kinases Mucosa Neonates Pain perception Permeability Proteins Rodents Small intestine Structure-function relationships Tumor necrosis factor-α Zonula occludens-1 protein γ-Interferon |
| title | In Vivo and In Vitro Antinociceptive Effect of Fagopyrum cymosum (Trev.) Meisn Extracts: A Possible Action by Recovering Intestinal Barrier Dysfunction |
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