Airway CD8+ T cells induced by pulmonary DNA immunization mediate protective anti-viral immunity
Vaccination strategies for protection against a number of respiratory pathogens must induce T-cell populations in both the pulmonary airways and peripheral lymphoid organs. In this study, we show that pulmonary immunization using plasmid DNA formulated with the polymer polyethyleneimine (PEI-DNA) in...
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| Veröffentlicht in: | Mucosal immunology 2013-01, Vol.6 (1), p.156-166 |
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| creator | Bivas-Benita, M Gillard, G O Bar, L White, K A Webby, R J Hovav, A-H Letvin, N L |
| description | Vaccination strategies for protection against a number of respiratory pathogens must induce T-cell populations in both the pulmonary airways and peripheral lymphoid organs. In this study, we show that pulmonary immunization using plasmid DNA formulated with the polymer polyethyleneimine (PEI-DNA) induced antigen-specific CD8
+
T cells in the airways that persisted long after antigen local clearance. The persistence of the cells was not mediated by local lymphocyte proliferation or persistent antigen presentation within the lung or airways. These vaccine-induced CD8
+
T cells effectively mediated protective immunity against respiratory challenges with vaccinia virus and influenza virus. Moreover, this protection was not dependent upon the recruitment of T cells from peripheral sites. These findings demonstrate that pulmonary immunization with PEI-DNA is an efficient approach for inducing robust pulmonary CD8
+
T-cell populations that are effective at protecting against respiratory pathogens. |
| doi_str_mv | 10.1038/mi.2012.59 |
| format | Article |
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T cells in the airways that persisted long after antigen local clearance. The persistence of the cells was not mediated by local lymphocyte proliferation or persistent antigen presentation within the lung or airways. These vaccine-induced CD8
+
T cells effectively mediated protective immunity against respiratory challenges with vaccinia virus and influenza virus. Moreover, this protection was not dependent upon the recruitment of T cells from peripheral sites. These findings demonstrate that pulmonary immunization with PEI-DNA is an efficient approach for inducing robust pulmonary CD8
+
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immunology</topic><topic>Antigens - genetics</topic><topic>Antigens - immunology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Epitopes, T-Lymphocyte - immunology</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>HIV Infections - immunology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - immunology</topic><topic>Humans</topic><topic>Immunization</topic><topic>Immunology</topic><topic>Lung - immunology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Mice</topic><topic>Orthomyxoviridae - immunology</topic><topic>Plasmids - genetics</topic><topic>Plasmids - immunology</topic><topic>Respiratory Mucosa - immunology</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><topic>Vaccinia virus - genetics</topic><topic>Vaccinia virus - immunology</topic><topic>Virus Diseases - immunology</topic><topic>Viruses - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bivas-Benita, M</creatorcontrib><creatorcontrib>Gillard, G O</creatorcontrib><creatorcontrib>Bar, L</creatorcontrib><creatorcontrib>White, K A</creatorcontrib><creatorcontrib>Webby, R J</creatorcontrib><creatorcontrib>Hovav, A-H</creatorcontrib><creatorcontrib>Letvin, N L</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Mucosal immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bivas-Benita, M</au><au>Gillard, G O</au><au>Bar, L</au><au>White, K A</au><au>Webby, R J</au><au>Hovav, A-H</au><au>Letvin, N L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Airway CD8+ T cells induced by pulmonary DNA immunization mediate protective anti-viral immunity</atitle><jtitle>Mucosal immunology</jtitle><stitle>Mucosal Immunol</stitle><addtitle>Mucosal Immunol</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>6</volume><issue>1</issue><spage>156</spage><epage>166</epage><pages>156-166</pages><issn>1933-0219</issn><eissn>1935-3456</eissn><abstract>Vaccination strategies for protection against a number of respiratory pathogens must induce T-cell populations in both the pulmonary airways and peripheral lymphoid organs. In this study, we show that pulmonary immunization using plasmid DNA formulated with the polymer polyethyleneimine (PEI-DNA) induced antigen-specific CD8
+
T cells in the airways that persisted long after antigen local clearance. The persistence of the cells was not mediated by local lymphocyte proliferation or persistent antigen presentation within the lung or airways. These vaccine-induced CD8
+
T cells effectively mediated protective immunity against respiratory challenges with vaccinia virus and influenza virus. Moreover, this protection was not dependent upon the recruitment of T cells from peripheral sites. These findings demonstrate that pulmonary immunization with PEI-DNA is an efficient approach for inducing robust pulmonary CD8
+
T-cell populations that are effective at protecting against respiratory pathogens.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>22806099</pmid><doi>10.1038/mi.2012.59</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1933-0219 |
| ispartof | Mucosal immunology, 2013-01, Vol.6 (1), p.156-166 |
| issn | 1933-0219 1935-3456 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 631/250/1619/554/1834 631/250/24/590/1991 631/250/251/1567 692/699/255/2514 Allergology Animals Antibodies Antigen Presentation - immunology Antigens - genetics Antigens - immunology Biomedical and Life Sciences Biomedicine CD8-Positive T-Lymphocytes - immunology Epitopes, T-Lymphocyte - immunology Female Gastroenterology HIV Infections - immunology HIV-1 - genetics HIV-1 - immunology Humans Immunization Immunology Lung - immunology Lymphocyte Activation - immunology Mice Orthomyxoviridae - immunology Plasmids - genetics Plasmids - immunology Respiratory Mucosa - immunology Vaccines, DNA - administration & dosage Vaccines, DNA - genetics Vaccines, DNA - immunology Vaccinia virus - genetics Vaccinia virus - immunology Virus Diseases - immunology Viruses - immunology |
| title | Airway CD8+ T cells induced by pulmonary DNA immunization mediate protective anti-viral immunity |
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