Effects of site-directed mutagenesis in the N-terminal domain of thermolysin on its stabilization

The thermolysin variant G8C/N60C/S65P in which the triple mutation in the N-terminal domain, Gly8→Cys/Asn60→Cys/Ser65→Pro, is undertaken increases stability [Yasukawa, K. and Inouye, K. (2007) Improving the activity and stability of thermolysin by site-directed mutagenesis. Biochim. Biophys. Acta 17...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 2013-01, Vol.153 (1), p.85-92
Hauptverfasser:	Kawasaki, Yuichi, Yasukawa, Kiyoshi, Inouye, Kuniyo
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Bacillus - enzymology Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding Sites Calcium Chloride - chemistry Circular Dichroism Cystine - analysis Enzyme Stability Hot Temperature - adverse effects Indicators and Reagents - chemistry Kinetics Models, Molecular Mutagenesis, Site-Directed Mutant Proteins - chemistry Mutant Proteins - metabolism Osmolar Concentration Protein Denaturation Protein Structure, Tertiary Proteolysis Recombinant Proteins - chemistry Recombinant Proteins - metabolism Regular Papers Thermolysin - chemistry Thermolysin - genetics Thermolysin - metabolism
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