Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation

Gene downregulation by antisense morpholino oligonucleotides (MOs) is achieved by either hybridization around the translation initiation codon or by targeting the splice donor site. In the present study, an antisense MO method is introduced that uses a 25-mer MO against a region at least 40-nt upstr...

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Veröffentlicht in:	Nucleic acids research 2012-12, Vol.40 (22), p.e173-e173
Hauptverfasser:	Wada, Tadashi, Hara, Masatoshi, Taneda, Takuya, Qingfu, Cao, Takata, Ryouhei, Moro, Kanako, Takeda, Kei, Kishimoto, Takeo, Handa, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	3' Untranslated Regions Animals Asterina - genetics Asterina - metabolism Asteroidea Cyclin B - genetics Cyclin B - metabolism Cyclin-Dependent Kinase 9 - antagonists & inhibitors Cyclin-Dependent Kinase 9 - genetics Danio rerio Down-Regulation Freshwater Gene Expression Regulation Gene Knockdown Techniques Injections Marine Methods Online Morpholinos - administration & dosage Morpholinos - pharmacology Oligonucleotides, Antisense - administration & dosage Oligonucleotides, Antisense - pharmacology Oocytes - drug effects Oocytes - metabolism Poly A - metabolism Polyadenylation - drug effects Protein Biosynthesis - drug effects RNA, Messenger, Stored - chemistry RNA, Messenger, Stored - metabolism Zebrafish - embryology Zebrafish - genetics Zebrafish - metabolism Zebrafish Proteins - antagonists & inhibitors Zebrafish Proteins - genetics
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