Expression of β-adrenergic receptors in pediatric malignant brain tumors
β-adrenergic receptors (β-ARs) are G protein-coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by m...
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| Veröffentlicht in: | Oncology letters 2013-01, Vol.5 (1), p.221-225 |
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| creator | SARDI, IACOPO GIUNTI, LAURA BRESCI, CECILIA BUCCOLIERO, ANNA MARIA DEGL'INNOCENTI, DUCCIO CARDELLICCHIO, STEFANIA BARONI, GIANNA CASTIGLIONE, FRANCESCA ROS, MARTINA DA FIORINI, PATRIZIO GIGLIO, SABRINA GENITORI, LORENZO ARICÒ, MAURIZIO FILIPPI, LUCA |
| description | β-adrenergic receptors (β-ARs) are G protein-coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, −9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines. |
| doi_str_mv | 10.3892/ol.2012.989 |
| format | Article |
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In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, −9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2012.989</identifier><identifier>PMID: 23255924</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>anaplastic ependymoma ; Angiogenesis ; Binding sites ; Brain cancer ; brain tumor cell lines ; Chemotherapy ; Gene expression ; glioblastoma multiforme ; Kinases ; Ligands ; medulloblastoma ; Metastasis ; Oncology ; Patients ; Pediatrics ; Polyclonal antibodies ; Prostate ; Proteins ; Radiation therapy ; Signal transduction ; Studies ; Tumors ; β-adrenergic receptors</subject><ispartof>Oncology letters, 2013-01, Vol.5 (1), p.221-225</ispartof><rights>Copyright © 2013, Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2013</rights><rights>Copyright © 2013, Spandidos Publications 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-bef824708d8e374b5edd63ae64f13225231676fd7e27e83e7a7923902a74d3723</citedby><cites>FETCH-LOGICAL-c440t-bef824708d8e374b5edd63ae64f13225231676fd7e27e83e7a7923902a74d3723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525361/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525361/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,5556,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23255924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SARDI, IACOPO</creatorcontrib><creatorcontrib>GIUNTI, LAURA</creatorcontrib><creatorcontrib>BRESCI, CECILIA</creatorcontrib><creatorcontrib>BUCCOLIERO, ANNA MARIA</creatorcontrib><creatorcontrib>DEGL'INNOCENTI, DUCCIO</creatorcontrib><creatorcontrib>CARDELLICCHIO, STEFANIA</creatorcontrib><creatorcontrib>BARONI, GIANNA</creatorcontrib><creatorcontrib>CASTIGLIONE, FRANCESCA</creatorcontrib><creatorcontrib>ROS, MARTINA DA</creatorcontrib><creatorcontrib>FIORINI, PATRIZIO</creatorcontrib><creatorcontrib>GIGLIO, SABRINA</creatorcontrib><creatorcontrib>GENITORI, LORENZO</creatorcontrib><creatorcontrib>ARICÒ, MAURIZIO</creatorcontrib><creatorcontrib>FILIPPI, LUCA</creatorcontrib><title>Expression of β-adrenergic receptors in pediatric malignant brain tumors</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>β-adrenergic receptors (β-ARs) are G protein-coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, −9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines.</description><subject>anaplastic ependymoma</subject><subject>Angiogenesis</subject><subject>Binding sites</subject><subject>Brain cancer</subject><subject>brain tumor cell lines</subject><subject>Chemotherapy</subject><subject>Gene expression</subject><subject>glioblastoma multiforme</subject><subject>Kinases</subject><subject>Ligands</subject><subject>medulloblastoma</subject><subject>Metastasis</subject><subject>Oncology</subject><subject>Patients</subject><subject>Pediatrics</subject><subject>Polyclonal antibodies</subject><subject>Prostate</subject><subject>Proteins</subject><subject>Radiation therapy</subject><subject>Signal transduction</subject><subject>Studies</subject><subject>Tumors</subject><subject>β-adrenergic receptors</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkd1qFTEUhYMottReeS8DghRkjsnO79wIUqoWCt7odciZ7DmmzCRjMlP0tXyQPpM5nHr82TfZZH8s1mIR8pzRDTcdvEnjBiiDTWe6R-SU6Q5aRg08Pu5anJDzUm5pHamYMeopOQEOUnYgTsn11fc5YykhxSYNzf3P1vmMEfMu9E3GHucl5dKE2Mzog1ty_Z7cGHbRxaXZZlcvyzpV5hl5Mrix4PnDe0a-vL_6fPmxvfn04fry3U3bC0GXdouDAaGp8Qa5FluJ3ivuUImBcQAJnCmtBq8RNBqO2tUcvKPgtPBcAz8jbw-687qd0PcYl-xGO-cwufzDJhfsv5cYvtpdurNcguSKVYGLB4Gcvq1YFjuF0uM4uohpLZYZUFKJTqmKvvwPvU1rjjWeZR0HxRWwvaPXB6rPqZSMw9EMo3bfkk2j3bdka0uVfvG3_yP7u5MKvDoAZXbRB5_Kn3BjS2VLWUsBGP8FWsyaIg</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>SARDI, IACOPO</creator><creator>GIUNTI, LAURA</creator><creator>BRESCI, CECILIA</creator><creator>BUCCOLIERO, ANNA MARIA</creator><creator>DEGL'INNOCENTI, DUCCIO</creator><creator>CARDELLICCHIO, STEFANIA</creator><creator>BARONI, GIANNA</creator><creator>CASTIGLIONE, FRANCESCA</creator><creator>ROS, MARTINA DA</creator><creator>FIORINI, PATRIZIO</creator><creator>GIGLIO, SABRINA</creator><creator>GENITORI, LORENZO</creator><creator>ARICÒ, MAURIZIO</creator><creator>FILIPPI, LUCA</creator><general>D.A. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SARDI, IACOPO</au><au>GIUNTI, LAURA</au><au>BRESCI, CECILIA</au><au>BUCCOLIERO, ANNA MARIA</au><au>DEGL'INNOCENTI, DUCCIO</au><au>CARDELLICCHIO, STEFANIA</au><au>BARONI, GIANNA</au><au>CASTIGLIONE, FRANCESCA</au><au>ROS, MARTINA DA</au><au>FIORINI, PATRIZIO</au><au>GIGLIO, SABRINA</au><au>GENITORI, LORENZO</au><au>ARICÒ, MAURIZIO</au><au>FILIPPI, LUCA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of β-adrenergic receptors in pediatric malignant brain tumors</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>5</volume><issue>1</issue><spage>221</spage><epage>225</epage><pages>221-225</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>β-adrenergic receptors (β-ARs) are G protein-coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, −9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>23255924</pmid><doi>10.3892/ol.2012.989</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-1074 |
| ispartof | Oncology letters, 2013-01, Vol.5 (1), p.221-225 |
| issn | 1792-1074 1792-1082 |
| language | eng |
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| source | Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | anaplastic ependymoma Angiogenesis Binding sites Brain cancer brain tumor cell lines Chemotherapy Gene expression glioblastoma multiforme Kinases Ligands medulloblastoma Metastasis Oncology Patients Pediatrics Polyclonal antibodies Prostate Proteins Radiation therapy Signal transduction Studies Tumors β-adrenergic receptors |
| title | Expression of β-adrenergic receptors in pediatric malignant brain tumors |
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