Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a major glycoglycerolipid from spinach, selectively inhibits the activities of mammalian replicative DNA polymerases (α, δ and ε). However, the function of MGDG following ingestion is not well-known. In the present study, sp...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2013-01, Vol.5 (1), p.17-22 |
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| creator | MAEDA, NAOKI KOKAI, YASUO HADA, TAKAHIKO YOSHIDA, HIROMI MIZUSHINA, YOSHIYUKI |
| description | Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a major glycoglycerolipid from spinach, selectively inhibits the activities of mammalian replicative DNA polymerases (α, δ and ε). However, the function of MGDG following ingestion is not well-known. In the present study, spinach MGDG suppressed the proliferation of Colon26 mouse colon cancer cells with an LD50 of 24 μg/ml in vitro. γ-cyclodextrin (CD)-MGDG complex was prepared and administered orally following Colon26 mouse tumor adhesion for 26 days. It was observed that 20 mg/kg equivalent (eq.) of the CD-MGDG complex reduced tumor volume by ∼60% compared with that of the vehicle-treated controls. In immunohistochemical analysis, the CD-MGDG complex group showed a decreased number of proliferating cell nuclear antigen (PCNA)-positive cells and reduction of mitosis in the tumor cells compared with the control group. In addition, the CD-MGDG complex increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive apoptotic cells and inhibited CD31-positive tumor blood vessel growth significantly. These results suggest that MGDG has the potential for cancer prevention and health promotion. |
| doi_str_mv | 10.3892/etm.2012.792 |
| format | Article |
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However, the function of MGDG following ingestion is not well-known. In the present study, spinach MGDG suppressed the proliferation of Colon26 mouse colon cancer cells with an LD50 of 24 μg/ml in vitro. γ-cyclodextrin (CD)-MGDG complex was prepared and administered orally following Colon26 mouse tumor adhesion for 26 days. It was observed that 20 mg/kg equivalent (eq.) of the CD-MGDG complex reduced tumor volume by ∼60% compared with that of the vehicle-treated controls. In immunohistochemical analysis, the CD-MGDG complex group showed a decreased number of proliferating cell nuclear antigen (PCNA)-positive cells and reduction of mitosis in the tumor cells compared with the control group. In addition, the CD-MGDG complex increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive apoptotic cells and inhibited CD31-positive tumor blood vessel growth significantly. These results suggest that MGDG has the potential for cancer prevention and health promotion.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2012.792</identifier><identifier>PMID: 23251235</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiogenesis ; anti-proliferation ; antitumor ; Apoptosis ; Cancer ; Cell growth ; Colorectal cancer ; Deoxyribonucleic acid ; DNA ; DNA polymerase ; Fatty acids ; Glycerol ; Laboratory animals ; Mitochondrial DNA ; monogalactosyl diacylglycerol ; Rodents ; Spinach ; Studies ; Tumors ; γ-cyclodextrin</subject><ispartof>Experimental and therapeutic medicine, 2013-01, Vol.5 (1), p.17-22</ispartof><rights>Copyright © 2013, Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2013</rights><rights>Copyright © 2013, Spandidos Publications 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-6b71f4b4978eac1a5c38b698afed3a2ddf16bab7cdd97b86e7bff1de0f544863</citedby><cites>FETCH-LOGICAL-c510t-6b71f4b4978eac1a5c38b698afed3a2ddf16bab7cdd97b86e7bff1de0f544863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524182/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524182/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23251235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MAEDA, NAOKI</creatorcontrib><creatorcontrib>KOKAI, YASUO</creatorcontrib><creatorcontrib>HADA, TAKAHIKO</creatorcontrib><creatorcontrib>YOSHIDA, HIROMI</creatorcontrib><creatorcontrib>MIZUSHINA, YOSHIYUKI</creatorcontrib><title>Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a major glycoglycerolipid from spinach, selectively inhibits the activities of mammalian replicative DNA polymerases (α, δ and ε). 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These results suggest that MGDG has the potential for cancer prevention and health promotion.</description><subject>Angiogenesis</subject><subject>anti-proliferation</subject><subject>antitumor</subject><subject>Apoptosis</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Colorectal cancer</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA polymerase</subject><subject>Fatty acids</subject><subject>Glycerol</subject><subject>Laboratory animals</subject><subject>Mitochondrial DNA</subject><subject>monogalactosyl diacylglycerol</subject><subject>Rodents</subject><subject>Spinach</subject><subject>Studies</subject><subject>Tumors</subject><subject>γ-cyclodextrin</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkUtrGzEURkVpSUKSXdZF0EW76Lh6jGY0m0IJTRsIZJO90NNW0MOVZhr87ytjx7TV5gru0Xd1OQDcYLSifCJf7BxXBGGyGifyBlzgVjqMMHt7vKOJ43NwXeszaocNmHN2Bs4JJQwTyi6AfSwyQGmiT77ORc4-J5gdjDnltQxSz7nuAjRe6l1Yh522JQfoSo6wbn2SegN92njl5wp1Du3xvMRc4Lrkl3nfg9FrewXeORmqvT7WS_B09_3p9mf38Pjj_vbbQ6cZRnM3qBG7XvXTyK3UWDJNuRomLp01VBJjHB6UVKM2ZhoVH-yonMPGIsf6ng_0Enw9xG4XFa3RNrWNgtgWH2XZiSy9-LeT_Eas829BGekxJy3g0zGg5F-LrbOIvmobgkw2L1U0ZmCMs2E_68N_6HNeSmrbCTzR5gDRcWrU5wOlS661WHf6DEZib1A0g2JvUDRdDX__9wIn-NVXAz4egLqVyXiT64lpQR1iHcIdQnikfwBuP6eD</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>MAEDA, NAOKI</creator><creator>KOKAI, YASUO</creator><creator>HADA, TAKAHIKO</creator><creator>YOSHIDA, HIROMI</creator><creator>MIZUSHINA, YOSHIYUKI</creator><general>D.A. 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However, the function of MGDG following ingestion is not well-known. In the present study, spinach MGDG suppressed the proliferation of Colon26 mouse colon cancer cells with an LD50 of 24 μg/ml in vitro. γ-cyclodextrin (CD)-MGDG complex was prepared and administered orally following Colon26 mouse tumor adhesion for 26 days. It was observed that 20 mg/kg equivalent (eq.) of the CD-MGDG complex reduced tumor volume by ∼60% compared with that of the vehicle-treated controls. In immunohistochemical analysis, the CD-MGDG complex group showed a decreased number of proliferating cell nuclear antigen (PCNA)-positive cells and reduction of mitosis in the tumor cells compared with the control group. In addition, the CD-MGDG complex increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive apoptotic cells and inhibited CD31-positive tumor blood vessel growth significantly. These results suggest that MGDG has the potential for cancer prevention and health promotion.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>23251235</pmid><doi>10.3892/etm.2012.792</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central |
| subjects | Angiogenesis anti-proliferation antitumor Apoptosis Cancer Cell growth Colorectal cancer Deoxyribonucleic acid DNA DNA polymerase Fatty acids Glycerol Laboratory animals Mitochondrial DNA monogalactosyl diacylglycerol Rodents Spinach Studies Tumors γ-cyclodextrin |
| title | Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice |
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