Tracking transmitter-gated P2X cation channel activation in vitro and in vivo

We present a noninvasive approach to track activation of ATP-gated P2X receptors and potentially other transmitter-gated cation channels that show calcium fluxes. We genetically engineered rat P2X receptors to carry calcium sensors near the channel pore and tested this as a reporter for P2X 2 recept...

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Veröffentlicht in:	Nature methods 2008-01, Vol.5 (1), p.87-93
Hauptverfasser:	Richler, Esther, Chaumont, Severine, Shigetomi, Eiji, Sagasti, Alvaro, Khakh, Baljit S
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine triphosphatase ATP Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Calcium Calcium - metabolism Cell receptors Energy transfer Energy transformation Fluorescence Fluorescence Resonance Energy Transfer - methods Ion Channel Gating - physiology Ion channels Life Sciences Neurons - physiology Physiology Proteomics Receptors, Purinergic P2 - metabolism Receptors, Purinergic P2X Research methodology Resonance Rodents Sensors Transgenic animals
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