Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel
Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence o...
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| Veröffentlicht in: | Enzyme Research 2012, Vol.2012 (2012), p.13-19 |
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| creator | Saravanan, P. Viruthagiri, T. Muthuvelayudham, R. |
| description | Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL. |
| doi_str_mv | 10.1155/2012/157643 |
| format | Article |
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The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.</description><identifier>ISSN: 2090-0414</identifier><identifier>ISSN: 2090-0406</identifier><identifier>EISSN: 2090-0414</identifier><identifier>DOI: 10.1155/2012/157643</identifier><identifier>PMID: 23304453</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Limiteds</publisher><subject>Cakes ; Cell culture ; Cellulase ; culture media ; endo-1,4-beta-glucanase ; enzyme activity ; Enzymes ; experimental design ; Flour ; Hypocrea jecorina ; Mangifera indica ; mango peels ; Nutrients ; response surface methodology ; screening ; soybean cake ; Soybeans ; Statistics ; Trichoderma reesei</subject><ispartof>Enzyme Research, 2012, Vol.2012 (2012), p.13-19</ispartof><rights>Copyright © 2012 P. Saravanan et al.</rights><rights>Copyright © 2012 P. Saravanan et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a5193-bdca22b9553d1297448c21617076229a414b8165ba0653c03f6cfd397623bfab3</citedby><cites>FETCH-LOGICAL-a5193-bdca22b9553d1297448c21617076229a414b8165ba0653c03f6cfd397623bfab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3523134/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3523134/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4009,27902,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23304453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Saboury, Ali-Akbar</contributor><creatorcontrib>Saravanan, P.</creatorcontrib><creatorcontrib>Viruthagiri, T.</creatorcontrib><creatorcontrib>Muthuvelayudham, R.</creatorcontrib><title>Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel</title><title>Enzyme Research</title><addtitle>Enzyme Res</addtitle><description>Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.</description><subject>Cakes</subject><subject>Cell culture</subject><subject>Cellulase</subject><subject>culture media</subject><subject>endo-1,4-beta-glucanase</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>experimental design</subject><subject>Flour</subject><subject>Hypocrea jecorina</subject><subject>Mangifera indica</subject><subject>mango peels</subject><subject>Nutrients</subject><subject>response surface methodology</subject><subject>screening</subject><subject>soybean cake</subject><subject>Soybeans</subject><subject>Statistics</subject><subject>Trichoderma reesei</subject><issn>2090-0414</issn><issn>2090-0406</issn><issn>2090-0414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><recordid>eNqNktFrFDEQxhdRbKl98lnJoyhnZzJJ9vIilFOrUvHA9jlkd7N3KXuba7Kr9L837bZH-3SGQCb5fnwwk68oXiN8RJTyhAPyE5SlEvSsOOSgYQYCxfNH9UFxnNIV5EU6b_WyOOBEIISkw2J9ut12vraDDz0LLfs95DIN-aVjn13yq561IbJh7dgyhmasH8CF67qxs8mx6oZdRF-vQ-PixrLoXHKeXSbfr9hP268CWzrXvSpetLZL7vj-PCouv365WHybnf86-744PZ9ZiZpmVVNbzistJTXIdSnEvOaosIRSca5t7qeao5KVBSWpBmpV3Taks0pVays6Kj5Nvtux2rimdv0QbWe20W9svDHBevNU6f3arMIfQ5ITksgG7-4NYrgeXRrMxqc6d2t7F8ZkuIA8O1Vy2Isiaa1BoZz_B4pSIUiB-1FeEs1Jap3RDxNax5BSdO2uTwRzmw9zmw8z5SPTbx-PZsc-pCED7ydg7fvG_vV73N5MsMuIa-0OFhIEqKz_mHTrox-8uQpj7PPPm2V2kcihBMA7x-yZjxIUVwBcPr0gmZyLf54B3ao</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Saravanan, P.</creator><creator>Viruthagiri, T.</creator><creator>Muthuvelayudham, R.</creator><general>Hindawi Limiteds</general><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><scope>188</scope><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>2012</creationdate><title>Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel</title><author>Saravanan, P. ; Viruthagiri, T. ; Muthuvelayudham, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a5193-bdca22b9553d1297448c21617076229a414b8165ba0653c03f6cfd397623bfab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Cakes</topic><topic>Cell culture</topic><topic>Cellulase</topic><topic>culture media</topic><topic>endo-1,4-beta-glucanase</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>experimental design</topic><topic>Flour</topic><topic>Hypocrea jecorina</topic><topic>Mangifera indica</topic><topic>mango peels</topic><topic>Nutrients</topic><topic>response surface methodology</topic><topic>screening</topic><topic>soybean cake</topic><topic>Soybeans</topic><topic>Statistics</topic><topic>Trichoderma reesei</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saravanan, P.</creatorcontrib><creatorcontrib>Viruthagiri, T.</creatorcontrib><creatorcontrib>Muthuvelayudham, R.</creatorcontrib><collection>Airiti Library</collection><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Enzyme Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saravanan, P.</au><au>Viruthagiri, T.</au><au>Muthuvelayudham, R.</au><au>Saboury, Ali-Akbar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel</atitle><jtitle>Enzyme Research</jtitle><addtitle>Enzyme Res</addtitle><date>2012</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>13</spage><epage>19</epage><pages>13-19</pages><issn>2090-0414</issn><issn>2090-0406</issn><eissn>2090-0414</eissn><abstract>Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Limiteds</pub><pmid>23304453</pmid><doi>10.1155/2012/157643</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cakes Cell culture Cellulase culture media endo-1,4-beta-glucanase enzyme activity Enzymes experimental design Flour Hypocrea jecorina Mangifera indica mango peels Nutrients response surface methodology screening soybean cake Soybeans Statistics Trichoderma reesei |
| title | Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel |
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