32P labeling of protein phosphorylation and metabolite association in the mitochondria matrix

Protein phosphorylations, as well as phosphate metabolite binding, are well characterized post-translational mechanisms that regulate enzyme activity in the cytosol, but remain poorly defined in mitochondria. Recently extensive matrix protein phosphorylation sites have been discovered but their func...

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Veröffentlicht in:	Methods in enzymology 2009, Vol.457, p.63-80
Hauptverfasser:	Aponte, Angel M, Phillips, Darci, Harris, Robert A, Blinova, Ksenia, French, Stephanie, Johnson, D Thor, Balaban, Robert S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Electrophoresis, Gel, Two-Dimensional Mitochondria, Heart - chemistry Mitochondria, Heart - metabolism Mitochondrial Proteins - analysis Mitochondrial Proteins - isolation & purification Mitochondrial Proteins - metabolism Phosphorus Radioisotopes - analysis Phosphorus Radioisotopes - metabolism Phosphorylation Pyruvate Dehydrogenase (Lipoamide)-Phosphatase - analysis Pyruvate Dehydrogenase (Lipoamide)-Phosphatase - metabolism Swine Time Factors
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