A mass spectrometry-based method to screen for α-amidated peptides

Amidation is a post‐translational modification found at the C‐terminus of ∼50% of all neuropeptide hormones. Cleavage of the Cα–N bond of a C‐terminal glycine yields the α‐amidated peptide in a reaction catalyzed by peptidylglycine α‐amidating monooxygenase (PAM). The mass of an α‐amidated peptide d...

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Veröffentlicht in:	Proteomics (Weinheim) 2012-01, Vol.12 (2), p.173-182
Hauptverfasser:	An, Zhenming, Chen, Yudan, Koomen, John M., Merkler, David J.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-MSH - chemistry Amides - chemistry Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Line, Tumor Chromatography, High Pressure Liquid - methods Databases, Protein Disulfiram - pharmacology Fundamental and applied biological sciences. Psychology Glycine - metabolism Mice Miscellaneous Mixed Function Oxygenases - metabolism Molecular Sequence Data Multienzyme Complexes - metabolism Peptides - chemistry Post-translational modification Protein Processing, Post-Translational Proteins Proteomics - methods Reproducibility of Results Search Engine Spectral pairing Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Technology Time Factors α-Amidated peptide
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