Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart
Nanoparticles of colloidal silver (AgNano) can influence gene expression. Concerning trials of AgNano application in poultry nutrition, it is useful to reveal whether they affect the expression of genes crucial for bird development. AgNano were administered to broiler chickens as a water solution in...
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| Veröffentlicht in: | Nanoscale research letters 2012-07, Vol.7 (1), p.418-418, Article 418 |
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| creator | Hotowy, Anna Sawosz, Ewa Pineda, Lane Sawosz, Filip Grodzik, Marta Chwalibog, André |
| description | Nanoparticles of colloidal silver (AgNano) can influence gene expression. Concerning trials of AgNano application in poultry nutrition, it is useful to reveal whether they affect the expression of genes crucial for bird development. AgNano were administered to broiler chickens as a water solution in two concentrations (10 and 20 ppm). After dissection of the birds, breast muscles and hearts were collected. Gene expression of
FGF2
and
VEGFA
on the mRNA and protein levels were evaluated using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay methods. The results for gene expression in the breast muscle revealed changes on the mRNA level (
FGF2
was up-regulated,
P
|
| doi_str_mv | 10.1186/1556-276X-7-418 |
| format | Article |
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FGF2
and
VEGFA
on the mRNA and protein levels were evaluated using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay methods. The results for gene expression in the breast muscle revealed changes on the mRNA level (
FGF2
was up-regulated,
P
< 0.05) but not on the protein level. In the heart, 20 ppm of silver nanoparticles in drinking water increased the expression of
VEGFA
(
P
< 0.05), at the same time decreasing
FGF2
expression both on the transcriptional and translational levels. Changes in the expression of these genes may lead to histological changes, but this needs to be proven using histological and immunohistochemical examination of tissues. In general, we showed that AgNano application in poultry feeding influences the expression of
FGF2
and
VEGFA
genes on the mRNA and protein levels in growing chicken.</description><identifier>ISSN: 1556-276X</identifier><identifier>ISSN: 1931-7573</identifier><identifier>EISSN: 1556-276X</identifier><identifier>DOI: 10.1186/1556-276X-7-418</identifier><identifier>PMID: 22827927</identifier><language>eng</language><publisher>New York: Springer New York</publisher><subject>Chemistry and Materials Science ; Materials Science ; Molecular Medicine ; Nano Express ; Nanochemistry ; Nanoscale Science and Technology ; Nanotechnology ; Nanotechnology and Microengineering</subject><ispartof>Nanoscale research letters, 2012-07, Vol.7 (1), p.418-418, Article 418</ispartof><rights>Hotowy et al.; licensee Springer. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright ©2012 Hotowy et al.; licensee Springer. 2012 Hotowy et al.; licensee Springer.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b493t-c89a4246c4cf60a24a0bc1e94d5f7162ca43a04f0e0ae3f7f1d5661c509109653</citedby><cites>FETCH-LOGICAL-b493t-c89a4246c4cf60a24a0bc1e94d5f7162ca43a04f0e0ae3f7f1d5661c509109653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507702/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507702/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22827927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hotowy, Anna</creatorcontrib><creatorcontrib>Sawosz, Ewa</creatorcontrib><creatorcontrib>Pineda, Lane</creatorcontrib><creatorcontrib>Sawosz, Filip</creatorcontrib><creatorcontrib>Grodzik, Marta</creatorcontrib><creatorcontrib>Chwalibog, André</creatorcontrib><title>Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart</title><title>Nanoscale research letters</title><addtitle>Nanoscale Res Lett</addtitle><addtitle>Nanoscale Res Lett</addtitle><description>Nanoparticles of colloidal silver (AgNano) can influence gene expression. Concerning trials of AgNano application in poultry nutrition, it is useful to reveal whether they affect the expression of genes crucial for bird development. AgNano were administered to broiler chickens as a water solution in two concentrations (10 and 20 ppm). After dissection of the birds, breast muscles and hearts were collected. Gene expression of
FGF2
and
VEGFA
on the mRNA and protein levels were evaluated using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay methods. The results for gene expression in the breast muscle revealed changes on the mRNA level (
FGF2
was up-regulated,
P
< 0.05) but not on the protein level. In the heart, 20 ppm of silver nanoparticles in drinking water increased the expression of
VEGFA
(
P
< 0.05), at the same time decreasing
FGF2
expression both on the transcriptional and translational levels. Changes in the expression of these genes may lead to histological changes, but this needs to be proven using histological and immunohistochemical examination of tissues. In general, we showed that AgNano application in poultry feeding influences the expression of
FGF2
and
VEGFA
genes on the mRNA and protein levels in growing chicken.</description><subject>Chemistry and Materials Science</subject><subject>Materials Science</subject><subject>Molecular Medicine</subject><subject>Nano Express</subject><subject>Nanochemistry</subject><subject>Nanoscale Science and Technology</subject><subject>Nanotechnology</subject><subject>Nanotechnology and Microengineering</subject><issn>1556-276X</issn><issn>1931-7573</issn><issn>1556-276X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp1kc1vFCEYh4nR2A89ezMcvUwLDAM7F5PadFeTJh78iDfyDvOyS52BFWaa-t_LunXTJvYE4ffkAd4fIW84O-N8oc5506hKaPWj0pXki2fk-HDy_MH-iJzkfMOY1Eyrl-RIiIXQrdDHZPzih1tMNECIW0iTtwNmCv3og88TJuzpFKndePsTAwXn0E70-9VqeUEh9HS5Wgq6xoAU77YJc_YxUB9olxDyRMc5F99fcoPF_oq8cDBkfH2_npJvy6uvlx-r68-rT5cX11Un23qq7KIFKaSy0jrFQEhgneXYyr5xmithQdbApGPIAGunHe8bpbhtWMtZq5r6lLzfe7dzN2JvMUwJBrNNfoT020Tw5nES_Mas462pG6Y1E0XwYS_ofHxC8DixcTS7cZvduI02pYwieXf_ihR_zZgnM_pscRggYJyz4UKw0gKvd-j5HrUp5pzQHa7izOya_o_87cMvHvh_1RaA7YFcorDGZG7inEIZ-5POPyBItY8</recordid><startdate>20120724</startdate><enddate>20120724</enddate><creator>Hotowy, Anna</creator><creator>Sawosz, Ewa</creator><creator>Pineda, Lane</creator><creator>Sawosz, Filip</creator><creator>Grodzik, Marta</creator><creator>Chwalibog, André</creator><general>Springer New York</general><general>BioMed Central Ltd</general><general>Springer</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120724</creationdate><title>Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart</title><author>Hotowy, Anna ; Sawosz, Ewa ; Pineda, Lane ; Sawosz, Filip ; Grodzik, Marta ; Chwalibog, André</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b493t-c89a4246c4cf60a24a0bc1e94d5f7162ca43a04f0e0ae3f7f1d5661c509109653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Chemistry and Materials Science</topic><topic>Materials Science</topic><topic>Molecular Medicine</topic><topic>Nano Express</topic><topic>Nanochemistry</topic><topic>Nanoscale Science and Technology</topic><topic>Nanotechnology</topic><topic>Nanotechnology and Microengineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hotowy, Anna</creatorcontrib><creatorcontrib>Sawosz, Ewa</creatorcontrib><creatorcontrib>Pineda, Lane</creatorcontrib><creatorcontrib>Sawosz, Filip</creatorcontrib><creatorcontrib>Grodzik, Marta</creatorcontrib><creatorcontrib>Chwalibog, André</creatorcontrib><collection>SpringerOpen</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nanoscale research letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hotowy, Anna</au><au>Sawosz, Ewa</au><au>Pineda, Lane</au><au>Sawosz, Filip</au><au>Grodzik, Marta</au><au>Chwalibog, André</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart</atitle><jtitle>Nanoscale research letters</jtitle><stitle>Nanoscale Res Lett</stitle><addtitle>Nanoscale Res Lett</addtitle><date>2012-07-24</date><risdate>2012</risdate><volume>7</volume><issue>1</issue><spage>418</spage><epage>418</epage><pages>418-418</pages><artnum>418</artnum><issn>1556-276X</issn><issn>1931-7573</issn><eissn>1556-276X</eissn><abstract>Nanoparticles of colloidal silver (AgNano) can influence gene expression. Concerning trials of AgNano application in poultry nutrition, it is useful to reveal whether they affect the expression of genes crucial for bird development. AgNano were administered to broiler chickens as a water solution in two concentrations (10 and 20 ppm). After dissection of the birds, breast muscles and hearts were collected. Gene expression of
FGF2
and
VEGFA
on the mRNA and protein levels were evaluated using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay methods. The results for gene expression in the breast muscle revealed changes on the mRNA level (
FGF2
was up-regulated,
P
< 0.05) but not on the protein level. In the heart, 20 ppm of silver nanoparticles in drinking water increased the expression of
VEGFA
(
P
< 0.05), at the same time decreasing
FGF2
expression both on the transcriptional and translational levels. Changes in the expression of these genes may lead to histological changes, but this needs to be proven using histological and immunohistochemical examination of tissues. In general, we showed that AgNano application in poultry feeding influences the expression of
FGF2
and
VEGFA
genes on the mRNA and protein levels in growing chicken.</abstract><cop>New York</cop><pub>Springer New York</pub><pmid>22827927</pmid><doi>10.1186/1556-276X-7-418</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Chemistry and Materials Science Materials Science Molecular Medicine Nano Express Nanochemistry Nanoscale Science and Technology Nanotechnology Nanotechnology and Microengineering |
| title | Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart |
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