Initial Characterization of Monoclonal Antibodies against Human Monocytes
Three monoclonal antibodies against human monocytes have been produced by somatic cell fusion. Extensive specificity analysis suggests that these antibodies react with most if not all human peripheral blood monocytes and not with highly purified T or B cells. Initial chemical characterization of the...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1980-11, Vol.77 (11), p.6764-6768 |
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| container_issue | 11 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 77 |
| creator | Ugolini, Valentina Nunez, Gabriel Smith, R. Graham Stastny, Peter Capra, J. Donald |
| description | Three monoclonal antibodies against human monocytes have been produced by somatic cell fusion. Extensive specificity analysis suggests that these antibodies react with most if not all human peripheral blood monocytes and not with highly purified T or B cells. Initial chemical characterization of the monocyte antigen recognized by two of these antibodies is presented. The molecule is a single polypeptide chain with an apparent molecular weight of 200,000. These reagents should prove useful in the clinical definition of disorders of monocyte differentiation, in studies of monocyte function, and in the elucidation of the genetics and structure of monocyte cell surface antigens. |
| doi_str_mv | 10.1073/pnas.77.11.6764 |
| format | Article |
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These reagents should prove useful in the clinical definition of disorders of monocyte differentiation, in studies of monocyte function, and in the elucidation of the genetics and structure of monocyte cell surface antigens.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.77.11.6764</identifier><identifier>PMID: 6935683</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Antigens, Surface - analysis ; B lymphocytes ; Blood ; Cell Line ; Cell lines ; Clone Cells - immunology ; Cultured cells ; Endothelial cells ; Endothelium - immunology ; Granulocytes ; Humans ; Hybrid Cells - immunology ; Lymphocytes - immunology ; Macrophages - immunology ; Membrane Proteins - immunology ; Mice ; Monoclonal antibodies ; Monocytes ; Monocytes - immunology ; T lymphocytes</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1980-11, Vol.77 (11), p.6764-6768</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-fc6166612fc2bdd4c686e45d541ef44ce298326f6b4768dd9ab3993d7eedd0603</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/77/11.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/9640$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/9640$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6935683$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ugolini, Valentina</creatorcontrib><creatorcontrib>Nunez, Gabriel</creatorcontrib><creatorcontrib>Smith, R. Graham</creatorcontrib><creatorcontrib>Stastny, Peter</creatorcontrib><creatorcontrib>Capra, J. Donald</creatorcontrib><title>Initial Characterization of Monoclonal Antibodies against Human Monocytes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Three monoclonal antibodies against human monocytes have been produced by somatic cell fusion. Extensive specificity analysis suggests that these antibodies react with most if not all human peripheral blood monocytes and not with highly purified T or B cells. Initial chemical characterization of the monocyte antigen recognized by two of these antibodies is presented. The molecule is a single polypeptide chain with an apparent molecular weight of 200,000. These reagents should prove useful in the clinical definition of disorders of monocyte differentiation, in studies of monocyte function, and in the elucidation of the genetics and structure of monocyte cell surface antigens.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens, Surface - analysis</subject><subject>B lymphocytes</subject><subject>Blood</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Clone Cells - immunology</subject><subject>Cultured cells</subject><subject>Endothelial cells</subject><subject>Endothelium - immunology</subject><subject>Granulocytes</subject><subject>Humans</subject><subject>Hybrid Cells - immunology</subject><subject>Lymphocytes - immunology</subject><subject>Macrophages - immunology</subject><subject>Membrane Proteins - immunology</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>Monocytes</subject><subject>Monocytes - immunology</subject><subject>T lymphocytes</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLFvEzEUhy0EKmlgRgygm8p06fPZ93weOlQR0EhFLDBbPtvXurrYwfYhyl_PRQkRXZg8fN_v-b0fIW8orCgIdrkLOq-EWFG6QoH8GVlQkLRGLuE5WQA0ou54w1-S85wfAEC2HZyRM5SsxY4tyGYTfPF6rNb3OmlTXPK_dfExVHGovsQQzRjDjK9D8X203uVK32kfcqlupq0OB-exuPyKvBj0mN3r47sk3z99_La-qW-_ft6sr29rwxFKPRikiEibwTS9tdxgh463tuXUDZwb18iONThgzwV21krdMymZFc5ZCwhsSa4Oc3dTv3XWuFCSHtUu-a1Ojypqr56S4O_VXfypWAtsvntJLo75FH9MLhe19dm4cdTBxSkrMW_SMqCzeHkQTYo5Jzec_qCg9uWrfflKCEWp2pc_J979u9rJP7Y98_dHvg_-pU8GfPivoIZpHIv7VWbz7cF8yCWmkyqRA_sD79OjTg</recordid><startdate>19801101</startdate><enddate>19801101</enddate><creator>Ugolini, Valentina</creator><creator>Nunez, Gabriel</creator><creator>Smith, R. 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Graham</creatorcontrib><creatorcontrib>Stastny, Peter</creatorcontrib><creatorcontrib>Capra, J. Donald</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ugolini, Valentina</au><au>Nunez, Gabriel</au><au>Smith, R. Graham</au><au>Stastny, Peter</au><au>Capra, J. Donald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Initial Characterization of Monoclonal Antibodies against Human Monocytes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1980-11-01</date><risdate>1980</risdate><volume>77</volume><issue>11</issue><spage>6764</spage><epage>6768</epage><pages>6764-6768</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Three monoclonal antibodies against human monocytes have been produced by somatic cell fusion. Extensive specificity analysis suggests that these antibodies react with most if not all human peripheral blood monocytes and not with highly purified T or B cells. Initial chemical characterization of the monocyte antigen recognized by two of these antibodies is presented. The molecule is a single polypeptide chain with an apparent molecular weight of 200,000. These reagents should prove useful in the clinical definition of disorders of monocyte differentiation, in studies of monocyte function, and in the elucidation of the genetics and structure of monocyte cell surface antigens.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6935683</pmid><doi>10.1073/pnas.77.11.6764</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1980-11, Vol.77 (11), p.6764-6768 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Antigens, Surface - analysis B lymphocytes Blood Cell Line Cell lines Clone Cells - immunology Cultured cells Endothelial cells Endothelium - immunology Granulocytes Humans Hybrid Cells - immunology Lymphocytes - immunology Macrophages - immunology Membrane Proteins - immunology Mice Monoclonal antibodies Monocytes Monocytes - immunology T lymphocytes |
| title | Initial Characterization of Monoclonal Antibodies against Human Monocytes |
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