Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity, broad dynamic range, and operational simplicity. These capabilities have been realized largely through incremental adaptations of native enzymes and substrates, originating from luminous organisms of divers...

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Veröffentlicht in:	ACS chemical biology 2012-11, Vol.7 (11), p.1848-1857
Hauptverfasser:	Hall, Mary P, Unch, James, Binkowski, Brock F, Valley, Michael P, Butler, Braeden L, Wood, Monika G, Otto, Paul, Zimmerman, Kristopher, Vidugiris, Gediminas, Machleidt, Thomas, Robers, Matthew B, Benink, Hélène A, Eggers, Christopher T, Slater, Michael R, Meisenheimer, Poncho L, Klaubert, Dieter H, Fan, Frank, Encell, Lance P, Wood, Keith V
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Crustacea - chemistry Crustacea - enzymology Crustacea - genetics Crustacea - metabolism Enzyme Stability Fireflies - enzymology Gene Expression Genes, Reporter Humans Luciferases - analysis Luciferases - genetics Luciferases - metabolism Luminescent Agents - analysis Luminescent Agents - metabolism Models, Molecular Oplophorus gracilirostris Photinus pyralis Protein Engineering Pyrazines - metabolism Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Renilla Renilla - enzymology Temperature
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