Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Through the ectopic expression of four transcription factors, Oct4, Klf4, Sox2 and cMyc, human somatic cells can be converted to a pluripotent state, generating so-called induced pluripotent stem cells (iPSCs)1-4. Patient-specific iPSCs lack the ethical concerns that surround embryonic stem cells (E...

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Veröffentlicht in:	Journal of Visualized Experiments 2012-10 (68)
Hauptverfasser:	Sommer, Andreia Gianotti, Rozelle, Sarah S., Sullivan, Spencer, Mills, Jason A., Park, Seon-Mi, Smith, Brenden W., Iyer, Amulya M., French, Deborah L., Kotton, Darrell N., Gadue, Paul, Murphy, George J., Mostoslavsky, Gustavo
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Surface - biosynthesis Genetic Vectors - genetics Humans Lentivirus - genetics Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - metabolism Leukocytes, Mononuclear - physiology Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Pluripotent Stem Cells - physiology Proteoglycans - biosynthesis Stage-Specific Embryonic Antigens - biosynthesis Stem Cell Biology Transduction, Genetic
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