Co-transcriptional Assembly of Chemically Modified RNA Nanoparticles Functionalized with siRNAs

We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2′-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in t...

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Veröffentlicht in:	Nano letters 2012-10, Vol.12 (10), p.5192-5195
Hauptverfasser:	Afonin, Kirill A, Kireeva, Maria, Grabow, Wade W, Kashlev, Mikhail, Jaeger, Luc, Shapiro, Bruce A
Format:	Artikel
Sprache:	eng
Schlagworte:	Assembly Blood Cell Line, Tumor Cross-disciplinary physics: materials science rheology DNA-Directed RNA Polymerases - metabolism Exact sciences and technology Human Humans Manganese ions Materials science Nanocrystalline materials Nanoparticles Nanoparticles - administration & dosage Nanoparticles - chemistry Nanoscale materials and structures: fabrication and characterization Nanotechnology Physics Pilots Polymerase Ribonucleic acids RNA - chemistry RNA - genetics RNA - metabolism RNA, Small Interfering - chemistry RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Serums Transcription, Genetic Viral Proteins - metabolism
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