Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cell

Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cell

DNA barcoding is an attractive technology as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here, we descri...

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Veröffentlicht in:Journal of the American Chemical Society 2012-11, Vol.134 (45), p.18499-18502
Hauptverfasser: Agasti, Sarit S., Liong, Monty, Peterson, Vanessa M., Lee, Hakho, Weissleder, Ralph
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container_end_page 18502
container_issue 45
container_start_page 18499
container_title Journal of the American Chemical Society
container_volume 134
creator Agasti, Sarit S.
Liong, Monty
Peterson, Vanessa M.
Lee, Hakho
Weissleder, Ralph
description DNA barcoding is an attractive technology as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here, we describe the development and validation of a photocleavable DNA barcode-antibody conjugate method for rapid, quantitative and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification and readout. As a proof of principle, we demonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.
doi_str_mv 10.1021/ja307689w
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title Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cell
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