Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip
Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma. Therefore, detection and genotyping of HPV are important to cervical-cancer screening. In this study, we have evaluated the efficacy of flow-through hybridization and gene chip...
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| description | Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma. Therefore, detection and genotyping of HPV are important to cervical-cancer screening. In this study, we have evaluated the efficacy of flow-through hybridization and gene chip (HybriMax) on HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH). 591 women were classified into 6 groups according to their histological diagnoses. The overall accordance rate on 13 types of HPV genotypes between HybriMax and HC-II were 92.5% and 100% in the cancer group. The overall accordance was excellent with the Kappa index (KI) of 0.814. The value of KI in each group was 0.750 (normal cytological diagnosis), 0.781 (chronic cervicitis), 0.80 (condyloma acuminatum), 0.755 (cervical intraepithelial neoplasia (CIN) I), 0.723 (CIN II), and 0.547 (CIN III) (0.75>KI>0.4, good; KI≥0.75, excellent). The 10 most common HPV subtype detected by HybriMax were 16, 52/58, 18, 33, 31, 81, 53, 68, and 66 in patients, and 16, 68, 18, 52, 58, 11, 53, 31/39, and 33 in normal controls. In conclusion, HybriMax is an efficient method for HPV genotyping and more suitable for clinical use. |
| doi_str_mv | 10.1155/2012/938780 |
| format | Article |
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C.</contributor><creatorcontrib>Tao, Pingping ; Zheng, Weiping ; Wang, Yungen ; Bian, Mei-lu ; Schmitt, F. C.</creatorcontrib><description>Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma. Therefore, detection and genotyping of HPV are important to cervical-cancer screening. In this study, we have evaluated the efficacy of flow-through hybridization and gene chip (HybriMax) on HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH). 591 women were classified into 6 groups according to their histological diagnoses. The overall accordance rate on 13 types of HPV genotypes between HybriMax and HC-II were 92.5% and 100% in the cancer group. The overall accordance was excellent with the Kappa index (KI) of 0.814. The value of KI in each group was 0.750 (normal cytological diagnosis), 0.781 (chronic cervicitis), 0.80 (condyloma acuminatum), 0.755 (cervical intraepithelial neoplasia (CIN) I), 0.723 (CIN II), and 0.547 (CIN III) (0.75>KI>0.4, good; KI≥0.75, excellent). The 10 most common HPV subtype detected by HybriMax were 16, 52/58, 18, 33, 31, 81, 53, 68, and 66 in patients, and 16, 68, 18, 52, 58, 11, 53, 31/39, and 33 in normal controls. In conclusion, HybriMax is an efficient method for HPV genotyping and more suitable for clinical use.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2012/938780</identifier><identifier>PMID: 23193367</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Adult ; Cancer ; Cervical cancer ; Cervical carcinoma ; Diagnosis ; DNA microarrays ; DNA, Viral - analysis ; DNA, Viral - genetics ; Female ; Genes ; Genotyping Techniques - methods ; Human papillomavirus ; Humans ; Hybridization ; In Situ Hybridization - methods ; Infection ; Middle Aged ; Oligonucleotide Array Sequence Analysis - methods ; Papillomaviridae - genetics ; Papillomavirus infections ; Sensitivity and Specificity ; Womens health ; Young Adult</subject><ispartof>BioMed research international, 2012-01, Vol.2012 (2012), p.1-6</ispartof><rights>Copyright © 2012 Pingping Tao et al.</rights><rights>COPYRIGHT 2012 John Wiley & Sons, Inc.</rights><rights>Copyright © 2012 Pingping Tao et al. Pingping Tao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2012 Pingping Tao et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c637t-188386e12d90fadea4964c3cd99c51a4feb1024b99c3dd40441a75a3e501bbcc3</citedby><cites>FETCH-LOGICAL-c637t-188386e12d90fadea4964c3cd99c51a4feb1024b99c3dd40441a75a3e501bbcc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495277/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495277/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23193367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Schmitt, F. C.</contributor><creatorcontrib>Tao, Pingping</creatorcontrib><creatorcontrib>Zheng, Weiping</creatorcontrib><creatorcontrib>Wang, Yungen</creatorcontrib><creatorcontrib>Bian, Mei-lu</creatorcontrib><title>Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma. Therefore, detection and genotyping of HPV are important to cervical-cancer screening. In this study, we have evaluated the efficacy of flow-through hybridization and gene chip (HybriMax) on HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH). 591 women were classified into 6 groups according to their histological diagnoses. The overall accordance rate on 13 types of HPV genotypes between HybriMax and HC-II were 92.5% and 100% in the cancer group. The overall accordance was excellent with the Kappa index (KI) of 0.814. The value of KI in each group was 0.750 (normal cytological diagnosis), 0.781 (chronic cervicitis), 0.80 (condyloma acuminatum), 0.755 (cervical intraepithelial neoplasia (CIN) I), 0.723 (CIN II), and 0.547 (CIN III) (0.75>KI>0.4, good; KI≥0.75, excellent). The 10 most common HPV subtype detected by HybriMax were 16, 52/58, 18, 33, 31, 81, 53, 68, and 66 in patients, and 16, 68, 18, 52, 58, 11, 53, 31/39, and 33 in normal controls. In conclusion, HybriMax is an efficient method for HPV genotyping and more suitable for clinical use.</description><subject>Adult</subject><subject>Cancer</subject><subject>Cervical cancer</subject><subject>Cervical carcinoma</subject><subject>Diagnosis</subject><subject>DNA microarrays</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Female</subject><subject>Genes</subject><subject>Genotyping Techniques - methods</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Hybridization</subject><subject>In Situ Hybridization - methods</subject><subject>Infection</subject><subject>Middle Aged</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Papillomaviridae - genetics</subject><subject>Papillomavirus infections</subject><subject>Sensitivity and Specificity</subject><subject>Womens health</subject><subject>Young Adult</subject><issn>1110-7243</issn><issn>2314-6133</issn><issn>1110-7251</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkt9r1TAUx4sobk6ffFYKvojSLSdpm-ZFmBe3KwwVnL6GNDltM3qTa9NuXP96c-m8OrngyEN-fc4Xzvl-k-Q5kGOAojihBOiJYBWvyIPkEABIxmkBD3fnnB0kT0K4IgR4VYrHyQFlIBgr-WHy6Su6YEd7jenyy_f0HJ0fN2vr2vS9CmhS79Kxw_Ss9zfZZTf4qe3S5aYerLE_1Wjjt3JmW4bporPrp8mjRvUBn93uR8m3sw-Xi2V28fn84-L0ItMl42MGVcWqEoEaQRplUOWizDXTRghdgMobrIHQvI5XZkxO8hwULxTDgkBda82Oknez7nqqV2g0unFQvVwPdqWGjfTKyrs_znay9deS5aKgnEeB17cCg_8xYRjlygaNfa8c-ilIoAJKShgt74EWnMcGaHEPlALQqipZRF_9g175aXBxaJGqokGUcvhDtapHaV3jYzd6KypPGROEkOh0pLI9VBtNia17h42Nz3f44z18XAZXVu8teDsX6MGHMGCzmzQQuQ2h3IZQziGM9Mu_zdmxv1MXgTcz0Fln1I39j9qLGcaIYKN2cEEEF4T9AoRQ6Uo</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Tao, Pingping</creator><creator>Zheng, Weiping</creator><creator>Wang, Yungen</creator><creator>Bian, Mei-lu</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip</title><author>Tao, Pingping ; Zheng, Weiping ; Wang, Yungen ; Bian, Mei-lu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c637t-188386e12d90fadea4964c3cd99c51a4feb1024b99c3dd40441a75a3e501bbcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Cancer</topic><topic>Cervical cancer</topic><topic>Cervical carcinoma</topic><topic>Diagnosis</topic><topic>DNA microarrays</topic><topic>DNA, Viral - 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip</atitle><jtitle>BioMed research international</jtitle><addtitle>J Biomed Biotechnol</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>1110-7243</issn><issn>2314-6133</issn><eissn>1110-7251</eissn><eissn>2314-6141</eissn><abstract>Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma. Therefore, detection and genotyping of HPV are important to cervical-cancer screening. In this study, we have evaluated the efficacy of flow-through hybridization and gene chip (HybriMax) on HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH). 591 women were classified into 6 groups according to their histological diagnoses. The overall accordance rate on 13 types of HPV genotypes between HybriMax and HC-II were 92.5% and 100% in the cancer group. The overall accordance was excellent with the Kappa index (KI) of 0.814. The value of KI in each group was 0.750 (normal cytological diagnosis), 0.781 (chronic cervicitis), 0.80 (condyloma acuminatum), 0.755 (cervical intraepithelial neoplasia (CIN) I), 0.723 (CIN II), and 0.547 (CIN III) (0.75>KI>0.4, good; KI≥0.75, excellent). The 10 most common HPV subtype detected by HybriMax were 16, 52/58, 18, 33, 31, 81, 53, 68, and 66 in patients, and 16, 68, 18, 52, 58, 11, 53, 31/39, and 33 in normal controls. In conclusion, HybriMax is an efficient method for HPV genotyping and more suitable for clinical use.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>23193367</pmid><doi>10.1155/2012/938780</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Cancer Cervical cancer Cervical carcinoma Diagnosis DNA microarrays DNA, Viral - analysis DNA, Viral - genetics Female Genes Genotyping Techniques - methods Human papillomavirus Humans Hybridization In Situ Hybridization - methods Infection Middle Aged Oligonucleotide Array Sequence Analysis - methods Papillomaviridae - genetics Papillomavirus infections Sensitivity and Specificity Womens health Young Adult |
| title | Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip |
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