Impact of Polymer-Bound Iodine on Fibronectin Adsorption and Osteoblast Cell Morphology in Radiopaque Medical Polymers: Tyrosine-Derived Polycarbonate Blends as a Model System
Imaging of polymer implants during surgical implantations is challenging in that most materials lack sufficient X-ray contrast. Synthetic derivatization with iodine serves to increase the scattering contrast but results in distinct physicochemical properties in the material which influence subsequen...
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| Veröffentlicht in: | Biomacromolecules 2009-09, Vol.10 (9), p.2418-2426 |
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| creator | Aamer, Khaled A Genson, Kirsten L Kohn, Joachim Becker, Matthew L |
| description | Imaging of polymer implants during surgical implantations is challenging in that most materials lack sufficient X-ray contrast. Synthetic derivatization with iodine serves to increase the scattering contrast but results in distinct physicochemical properties in the material which influence subsequent protein adsorption and cell morphology behavior. Herein we report the impact of increasing iodine inclusion on the cell morphology (cell area and shape) of MC3T3-E1 osteoblasts on a series of homopolymers and discrete blend thin films of poly(desaminotyrosyl tyrosine ethyl ester carbonate), poly(DTE carbonate), and an iodinated analogue poly(I2-DTE carbonate). Cell morphology is correlated to film chemical composition via measuring fibronectin (FN) adhesion protein adsorption profile on these films. FN exhibits up to 2-fold greater adsorption affinity for poly(I2-DTE carbonate) than (poly(DTE carbonate)). A correlation was established between cell area, roundness, and the measured FN adsorption profile on the blend films up to 75% by mass poly(I2-DTE carbonate). Data suggest that incorporation of iodine within the polymer backbone has a distinct impact on the way FN proteins adsorb to the surface and within the studied blend systems; the effect is composition dependent. |
| doi_str_mv | 10.1021/bm900327b |
| format | Article |
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Synthetic derivatization with iodine serves to increase the scattering contrast but results in distinct physicochemical properties in the material which influence subsequent protein adsorption and cell morphology behavior. Herein we report the impact of increasing iodine inclusion on the cell morphology (cell area and shape) of MC3T3-E1 osteoblasts on a series of homopolymers and discrete blend thin films of poly(desaminotyrosyl tyrosine ethyl ester carbonate), poly(DTE carbonate), and an iodinated analogue poly(I2-DTE carbonate). Cell morphology is correlated to film chemical composition via measuring fibronectin (FN) adhesion protein adsorption profile on these films. FN exhibits up to 2-fold greater adsorption affinity for poly(I2-DTE carbonate) than (poly(DTE carbonate)). A correlation was established between cell area, roundness, and the measured FN adsorption profile on the blend films up to 75% by mass poly(I2-DTE carbonate). Data suggest that incorporation of iodine within the polymer backbone has a distinct impact on the way FN proteins adsorb to the surface and within the studied blend systems; the effect is composition dependent.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm900327b</identifier><identifier>PMID: 19645443</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>3T3 Cells ; Adsorption ; Animals ; Applied sciences ; Biological and medical sciences ; Biological properties ; Cell Adhesion ; Cell Shape ; Exact sciences and technology ; Fibronectins - analysis ; Fibronectins - metabolism ; Iodine - chemistry ; Medical sciences ; Mice ; Organic polymers ; Osteoblasts - cytology ; Physicochemistry of polymers ; Polycarboxylate Cement ; Polymers - chemistry ; Properties and characterization ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; Tyrosine</subject><ispartof>Biomacromolecules, 2009-09, Vol.10 (9), p.2418-2426</ispartof><rights>Copyright © 2009 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a434t-44a20682891e074046c6ebe8d01868feedf02b4ca37ee64c7d5d97032d17b9203</citedby><cites>FETCH-LOGICAL-a434t-44a20682891e074046c6ebe8d01868feedf02b4ca37ee64c7d5d97032d17b9203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bm900327b$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bm900327b$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22077189$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19645443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aamer, Khaled A</creatorcontrib><creatorcontrib>Genson, Kirsten L</creatorcontrib><creatorcontrib>Kohn, Joachim</creatorcontrib><creatorcontrib>Becker, Matthew L</creatorcontrib><title>Impact of Polymer-Bound Iodine on Fibronectin Adsorption and Osteoblast Cell Morphology in Radiopaque Medical Polymers: Tyrosine-Derived Polycarbonate Blends as a Model System</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>Imaging of polymer implants during surgical implantations is challenging in that most materials lack sufficient X-ray contrast. Synthetic derivatization with iodine serves to increase the scattering contrast but results in distinct physicochemical properties in the material which influence subsequent protein adsorption and cell morphology behavior. Herein we report the impact of increasing iodine inclusion on the cell morphology (cell area and shape) of MC3T3-E1 osteoblasts on a series of homopolymers and discrete blend thin films of poly(desaminotyrosyl tyrosine ethyl ester carbonate), poly(DTE carbonate), and an iodinated analogue poly(I2-DTE carbonate). Cell morphology is correlated to film chemical composition via measuring fibronectin (FN) adhesion protein adsorption profile on these films. FN exhibits up to 2-fold greater adsorption affinity for poly(I2-DTE carbonate) than (poly(DTE carbonate)). A correlation was established between cell area, roundness, and the measured FN adsorption profile on the blend films up to 75% by mass poly(I2-DTE carbonate). Data suggest that incorporation of iodine within the polymer backbone has a distinct impact on the way FN proteins adsorb to the surface and within the studied blend systems; the effect is composition dependent.</description><subject>3T3 Cells</subject><subject>Adsorption</subject><subject>Animals</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biological properties</subject><subject>Cell Adhesion</subject><subject>Cell Shape</subject><subject>Exact sciences and technology</subject><subject>Fibronectins - analysis</subject><subject>Fibronectins - metabolism</subject><subject>Iodine - chemistry</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Organic polymers</subject><subject>Osteoblasts - cytology</subject><subject>Physicochemistry of polymers</subject><subject>Polycarboxylate Cement</subject><subject>Polymers - chemistry</subject><subject>Properties and characterization</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tyrosine</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkd1qFTEUhQdR7I9e-AKSGxEvRpNMzmTGC6E9Wj3QUtF6HfYke9qUTDImM4V5qr6isT2eKgiBhKyPtTZrF8ULRt8yytm7bmgprbjsHhX7bMXrUtSUP757r0opW7lXHKR0TSltK7F6WuyxthYrIar94nYzjKAnEnryNbhlwFgeh9kbsgnGeiTBkxPbxeBRT9aTI5NCHCebvyFD52nC0DlIE1mjc-Qsi1fBhcuFZPgbGBtG-DkjOUNjNbg_Gek9uVhiSDmh_IjR3qC5kzTELniYkBw79CYRyCe7GnTk-5LDhmfFkx5cwufb-7D4cfLpYv2lPD3_vFkfnZYgKjGVQgCndcObliGVgopa19hhYyhr6qZHND3lndBQScRaaGlWppW5Q8Nk13JaHRYf7n3HuRvQaPRTBKfGaAeIiwpg1b-Kt1fqMtyoSrSCyzYbvN4axJAbSJMabNK5JPAY5qRklaeqaiYy-eae1LmRFLHfpTCqfu9X7fab2Zd_j_VAbheagVdbAFIuvI_gtU07jnMqJWvaBw50Utdhjj63-Z_AX-9EvSg</recordid><startdate>20090914</startdate><enddate>20090914</enddate><creator>Aamer, Khaled A</creator><creator>Genson, Kirsten L</creator><creator>Kohn, Joachim</creator><creator>Becker, Matthew L</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090914</creationdate><title>Impact of Polymer-Bound Iodine on Fibronectin Adsorption and Osteoblast Cell Morphology in Radiopaque Medical Polymers: Tyrosine-Derived Polycarbonate Blends as a Model System</title><author>Aamer, Khaled A ; Genson, Kirsten L ; Kohn, Joachim ; Becker, Matthew L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a434t-44a20682891e074046c6ebe8d01868feedf02b4ca37ee64c7d5d97032d17b9203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>3T3 Cells</topic><topic>Adsorption</topic><topic>Animals</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biological properties</topic><topic>Cell Adhesion</topic><topic>Cell Shape</topic><topic>Exact sciences and technology</topic><topic>Fibronectins - analysis</topic><topic>Fibronectins - metabolism</topic><topic>Iodine - chemistry</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Organic polymers</topic><topic>Osteoblasts - cytology</topic><topic>Physicochemistry of polymers</topic><topic>Polycarboxylate Cement</topic><topic>Polymers - chemistry</topic><topic>Properties and characterization</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tyrosine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aamer, Khaled A</creatorcontrib><creatorcontrib>Genson, Kirsten L</creatorcontrib><creatorcontrib>Kohn, Joachim</creatorcontrib><creatorcontrib>Becker, Matthew L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomacromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aamer, Khaled A</au><au>Genson, Kirsten L</au><au>Kohn, Joachim</au><au>Becker, Matthew L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of Polymer-Bound Iodine on Fibronectin Adsorption and Osteoblast Cell Morphology in Radiopaque Medical Polymers: Tyrosine-Derived Polycarbonate Blends as a Model System</atitle><jtitle>Biomacromolecules</jtitle><addtitle>Biomacromolecules</addtitle><date>2009-09-14</date><risdate>2009</risdate><volume>10</volume><issue>9</issue><spage>2418</spage><epage>2426</epage><pages>2418-2426</pages><issn>1525-7797</issn><eissn>1526-4602</eissn><abstract>Imaging of polymer implants during surgical implantations is challenging in that most materials lack sufficient X-ray contrast. Synthetic derivatization with iodine serves to increase the scattering contrast but results in distinct physicochemical properties in the material which influence subsequent protein adsorption and cell morphology behavior. Herein we report the impact of increasing iodine inclusion on the cell morphology (cell area and shape) of MC3T3-E1 osteoblasts on a series of homopolymers and discrete blend thin films of poly(desaminotyrosyl tyrosine ethyl ester carbonate), poly(DTE carbonate), and an iodinated analogue poly(I2-DTE carbonate). Cell morphology is correlated to film chemical composition via measuring fibronectin (FN) adhesion protein adsorption profile on these films. FN exhibits up to 2-fold greater adsorption affinity for poly(I2-DTE carbonate) than (poly(DTE carbonate)). A correlation was established between cell area, roundness, and the measured FN adsorption profile on the blend films up to 75% by mass poly(I2-DTE carbonate). Data suggest that incorporation of iodine within the polymer backbone has a distinct impact on the way FN proteins adsorb to the surface and within the studied blend systems; the effect is composition dependent.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>19645443</pmid><doi>10.1021/bm900327b</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3T3 Cells Adsorption Animals Applied sciences Biological and medical sciences Biological properties Cell Adhesion Cell Shape Exact sciences and technology Fibronectins - analysis Fibronectins - metabolism Iodine - chemistry Medical sciences Mice Organic polymers Osteoblasts - cytology Physicochemistry of polymers Polycarboxylate Cement Polymers - chemistry Properties and characterization Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Tyrosine |
| title | Impact of Polymer-Bound Iodine on Fibronectin Adsorption and Osteoblast Cell Morphology in Radiopaque Medical Polymers: Tyrosine-Derived Polycarbonate Blends as a Model System |
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