Dicer-dependent and -independent Argonaute2 Protein Interaction Networks in Mammalian Cells
Argonaute (Ago) proteins interact with small regulatory RNAs such as microRNAs (miRNAs) and facilitate gene-silencing processes. miRNAs guide Ago proteins to specific mRNAs leading to translational silencing or mRNA decay. In order to understand the mechanistic details of miRNA function, it is impor...
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| Veröffentlicht in: | Molecular & cellular proteomics 2012-11, Vol.11 (11), p.1442-1456 |
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| container_title | Molecular & cellular proteomics |
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| creator | Frohn, Anne Eberl, H. Christian Stöhr, Julia Glasmacher, Elke Rüdel, Sabine Heissmeyer, Vigo Mann, Matthias Meister, Gunter |
| description | Argonaute (Ago) proteins interact with small regulatory RNAs such as microRNAs (miRNAs) and facilitate gene-silencing processes. miRNAs guide Ago proteins to specific mRNAs leading to translational silencing or mRNA decay. In order to understand the mechanistic details of miRNA function, it is important to characterize Ago protein interactors. Although several proteomic studies have been performed, it is not clear how the Ago interactome changes on miRNA or mRNA binding. Here, we report the analysis of Ago protein interactions in miRNA-containing and miRNA-depleted cells. Using stable isotope labeling in cell culture in conjunction with Dicer knock out mouse embryonic fibroblasts, we identify proteins that interact with Ago2 in the presence or the absence of Dicer. In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. Our proteomics approach provides a rich resource for further functional studies on the cellular roles of Ago proteins. |
| doi_str_mv | 10.1074/mcp.M112.017756 |
| format | Article |
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Christian ; Stöhr, Julia ; Glasmacher, Elke ; Rüdel, Sabine ; Heissmeyer, Vigo ; Mann, Matthias ; Meister, Gunter</creator><creatorcontrib>Frohn, Anne ; Eberl, H. Christian ; Stöhr, Julia ; Glasmacher, Elke ; Rüdel, Sabine ; Heissmeyer, Vigo ; Mann, Matthias ; Meister, Gunter</creatorcontrib><description>Argonaute (Ago) proteins interact with small regulatory RNAs such as microRNAs (miRNAs) and facilitate gene-silencing processes. miRNAs guide Ago proteins to specific mRNAs leading to translational silencing or mRNA decay. In order to understand the mechanistic details of miRNA function, it is important to characterize Ago protein interactors. Although several proteomic studies have been performed, it is not clear how the Ago interactome changes on miRNA or mRNA binding. Here, we report the analysis of Ago protein interactions in miRNA-containing and miRNA-depleted cells. Using stable isotope labeling in cell culture in conjunction with Dicer knock out mouse embryonic fibroblasts, we identify proteins that interact with Ago2 in the presence or the absence of Dicer. In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. 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Here, we report the analysis of Ago protein interactions in miRNA-containing and miRNA-depleted cells. Using stable isotope labeling in cell culture in conjunction with Dicer knock out mouse embryonic fibroblasts, we identify proteins that interact with Ago2 in the presence or the absence of Dicer. In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. 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Christian</creatorcontrib><creatorcontrib>Stöhr, Julia</creatorcontrib><creatorcontrib>Glasmacher, Elke</creatorcontrib><creatorcontrib>Rüdel, Sabine</creatorcontrib><creatorcontrib>Heissmeyer, Vigo</creatorcontrib><creatorcontrib>Mann, Matthias</creatorcontrib><creatorcontrib>Meister, Gunter</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular & cellular proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frohn, Anne</au><au>Eberl, H. 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| subjects | Animals Argonaute Proteins - metabolism Blotting, Western DEAD-box RNA Helicases - metabolism Embryo, Mammalian - cytology Fibroblasts - metabolism Mammals - metabolism Mice MicroRNAs - metabolism Protein Binding Protein Interaction Maps Reproducibility of Results Ribonuclease III - metabolism Ribonucleoproteins - metabolism |
| title | Dicer-dependent and -independent Argonaute2 Protein Interaction Networks in Mammalian Cells |
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