An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy
The various iterations of the HapMap Project and many genome-wide association studies (GWAS) have identified hundreds of potential genes involved in monogenic and multifactorial traits. We constructed an arrayed 115,000-member human genomic library in the PAC shuttle vector pJCPAC-Mam2 that can be p...
Gespeichert in:
| Veröffentlicht in: | Gene 2012-04, Vol.496 (2), p.103-109 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 109 |
|---|---|
| container_issue | 2 |
| container_start_page | 103 |
| container_title | Gene |
| container_volume | 496 |
| creator | Fuesler, John Nagahama, Yasunori Szulewski, Joseph Mundorff, Joshua Bireley, Stephanie Coren, Jonathon S. |
| description | The various iterations of the HapMap Project and many genome-wide association studies (GWAS) have identified hundreds of potential genes involved in monogenic and multifactorial traits. We constructed an arrayed 115,000-member human genomic library in the PAC shuttle vector pJCPAC-Mam2 that can be propagated in both bacterial and human cells. The library appears to represent a two-fold coverage of the human genome. Transient transfection of a p53-containing PAC clone into p53-null Saos-2 human osteosarcoma cells demonstrated that both p53 mRNA and protein were produced. Additionally, expression of the p53 protein triggered apoptosis in a subset of the Saos-2 cells. This library should serve as a valuable resource to validate potential disease genes identified by GWAS in human cell lines and in animal models. Also, individual library members could potentially be used for gene therapy trials for a variety of recessive disorders.
► Human genomic PAC library for functional genomics. ► p53-containing PAC clone is transcribed in p53 null Saos-2 cells. ► p53-containing PAC clone is translated in p53 null Saos-2 cells. ► p53-containing PAC clone causes apoptosis in p53 null Saos-2 cells. ► Human genomic PAC library for gene therapy. |
| doi_str_mv | 10.1016/j.gene.2012.01.011 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3488463</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378111912000406</els_id><sourcerecordid>922759507</sourcerecordid><originalsourceid>FETCH-LOGICAL-c487t-1ee9475e1ee0d2d5ee15af68a3aa6c44a19bf601540aa0b6dc5c197a1d8fe4533</originalsourceid><addsrcrecordid>eNp9kc9u1DAQxiNERZfCC3BAvtFLtrYTJ46EkFYryh8VwQHO1qw96XqV2IvtLNon4LXrsKWCS62RRmP_5pNnvqJ4xeiSUdZc7Za36HDJKeNLynKwJ8WCybYrKa3k02JBq1aWjLHuvHge447mIwR_VpxzzqXouFgUv1eOQAhwREO20wiOZE0_Wk0GuwkQjkR7F1OYdMqEdSRtkXxbrUncTikNSA6okw9k_3mdb8svMHLS5_qPCpa_rEECMXptIVnvSEyTsRgJODMzOOsF2B9fFGc9DBFf3ueL4sf1--_rj-XN1w-f1qubUteyTSVD7OpWYM7UcCMQmYC-kVABNLqugXWbvqFM1BSAbhqjhWZdC8zIHmtRVRfFu5PuftqMaDS6FGBQ-2DHPKzyYNX_L85u1a0_qKqWsm5mgTf3AsH_nDAmNdqocRjAoZ-i6jhvRSdom8nLR0nWtJzOLsiM8hOqg48xYP_wIUbV7LXaqXlbavZaUZaD5abX_47y0PLX3Ay8PQGYF3qwGFTUFp1GY0N2TRlvH9O_A3BnvYw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1672059258</pqid></control><display><type>article</type><title>An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Fuesler, John ; Nagahama, Yasunori ; Szulewski, Joseph ; Mundorff, Joshua ; Bireley, Stephanie ; Coren, Jonathon S.</creator><creatorcontrib>Fuesler, John ; Nagahama, Yasunori ; Szulewski, Joseph ; Mundorff, Joshua ; Bireley, Stephanie ; Coren, Jonathon S.</creatorcontrib><description>The various iterations of the HapMap Project and many genome-wide association studies (GWAS) have identified hundreds of potential genes involved in monogenic and multifactorial traits. We constructed an arrayed 115,000-member human genomic library in the PAC shuttle vector pJCPAC-Mam2 that can be propagated in both bacterial and human cells. The library appears to represent a two-fold coverage of the human genome. Transient transfection of a p53-containing PAC clone into p53-null Saos-2 human osteosarcoma cells demonstrated that both p53 mRNA and protein were produced. Additionally, expression of the p53 protein triggered apoptosis in a subset of the Saos-2 cells. This library should serve as a valuable resource to validate potential disease genes identified by GWAS in human cell lines and in animal models. Also, individual library members could potentially be used for gene therapy trials for a variety of recessive disorders.
► Human genomic PAC library for functional genomics. ► p53-containing PAC clone is transcribed in p53 null Saos-2 cells. ► p53-containing PAC clone is translated in p53 null Saos-2 cells. ► p53-containing PAC clone causes apoptosis in p53 null Saos-2 cells. ► Human genomic PAC library for gene therapy.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2012.01.011</identifier><identifier>PMID: 22285925</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>animal models ; Apoptosis ; Cell Line, Tumor ; chemistry ; Cloning, Molecular ; Functional genomics ; Gene therapy ; genes ; Genes, p53 ; Genetic Therapy ; Genetic Therapy - methods ; Genetic Vectors ; genetics ; Genome-wide association studies ; Genome-Wide Association Study ; Genome-Wide Association Study - methods ; genomic libraries ; Genomic Library ; Genomics ; Genomics - methods ; Green Fluorescent Proteins ; Green Fluorescent Proteins - chemistry ; HapMap Project ; human cell lines ; Humans ; messenger RNA ; metabolism ; methods ; osteosarcoma ; Osteosarcoma - metabolism ; Protein Biosynthesis ; Transcription, Genetic ; transfection ; Tumor Suppressor Protein p53 ; Tumor Suppressor Protein p53 - genetics</subject><ispartof>Gene, 2012-04, Vol.496 (2), p.103-109</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><rights>2012 Elsevier B.V. All rights reserved. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-1ee9475e1ee0d2d5ee15af68a3aa6c44a19bf601540aa0b6dc5c197a1d8fe4533</citedby><cites>FETCH-LOGICAL-c487t-1ee9475e1ee0d2d5ee15af68a3aa6c44a19bf601540aa0b6dc5c197a1d8fe4533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111912000406$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22285925$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fuesler, John</creatorcontrib><creatorcontrib>Nagahama, Yasunori</creatorcontrib><creatorcontrib>Szulewski, Joseph</creatorcontrib><creatorcontrib>Mundorff, Joshua</creatorcontrib><creatorcontrib>Bireley, Stephanie</creatorcontrib><creatorcontrib>Coren, Jonathon S.</creatorcontrib><title>An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy</title><title>Gene</title><addtitle>Gene</addtitle><description>The various iterations of the HapMap Project and many genome-wide association studies (GWAS) have identified hundreds of potential genes involved in monogenic and multifactorial traits. We constructed an arrayed 115,000-member human genomic library in the PAC shuttle vector pJCPAC-Mam2 that can be propagated in both bacterial and human cells. The library appears to represent a two-fold coverage of the human genome. Transient transfection of a p53-containing PAC clone into p53-null Saos-2 human osteosarcoma cells demonstrated that both p53 mRNA and protein were produced. Additionally, expression of the p53 protein triggered apoptosis in a subset of the Saos-2 cells. This library should serve as a valuable resource to validate potential disease genes identified by GWAS in human cell lines and in animal models. Also, individual library members could potentially be used for gene therapy trials for a variety of recessive disorders.
► Human genomic PAC library for functional genomics. ► p53-containing PAC clone is transcribed in p53 null Saos-2 cells. ► p53-containing PAC clone is translated in p53 null Saos-2 cells. ► p53-containing PAC clone causes apoptosis in p53 null Saos-2 cells. ► Human genomic PAC library for gene therapy.</description><subject>animal models</subject><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>chemistry</subject><subject>Cloning, Molecular</subject><subject>Functional genomics</subject><subject>Gene therapy</subject><subject>genes</subject><subject>Genes, p53</subject><subject>Genetic Therapy</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors</subject><subject>genetics</subject><subject>Genome-wide association studies</subject><subject>Genome-Wide Association Study</subject><subject>Genome-Wide Association Study - methods</subject><subject>genomic libraries</subject><subject>Genomic Library</subject><subject>Genomics</subject><subject>Genomics - methods</subject><subject>Green Fluorescent Proteins</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>HapMap Project</subject><subject>human cell lines</subject><subject>Humans</subject><subject>messenger RNA</subject><subject>metabolism</subject><subject>methods</subject><subject>osteosarcoma</subject><subject>Osteosarcoma - metabolism</subject><subject>Protein Biosynthesis</subject><subject>Transcription, Genetic</subject><subject>transfection</subject><subject>Tumor Suppressor Protein p53</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxiNERZfCC3BAvtFLtrYTJ46EkFYryh8VwQHO1qw96XqV2IvtLNon4LXrsKWCS62RRmP_5pNnvqJ4xeiSUdZc7Za36HDJKeNLynKwJ8WCybYrKa3k02JBq1aWjLHuvHge447mIwR_VpxzzqXouFgUv1eOQAhwREO20wiOZE0_Wk0GuwkQjkR7F1OYdMqEdSRtkXxbrUncTikNSA6okw9k_3mdb8svMHLS5_qPCpa_rEECMXptIVnvSEyTsRgJODMzOOsF2B9fFGc9DBFf3ueL4sf1--_rj-XN1w-f1qubUteyTSVD7OpWYM7UcCMQmYC-kVABNLqugXWbvqFM1BSAbhqjhWZdC8zIHmtRVRfFu5PuftqMaDS6FGBQ-2DHPKzyYNX_L85u1a0_qKqWsm5mgTf3AsH_nDAmNdqocRjAoZ-i6jhvRSdom8nLR0nWtJzOLsiM8hOqg48xYP_wIUbV7LXaqXlbavZaUZaD5abX_47y0PLX3Ay8PQGYF3qwGFTUFp1GY0N2TRlvH9O_A3BnvYw</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Fuesler, John</creator><creator>Nagahama, Yasunori</creator><creator>Szulewski, Joseph</creator><creator>Mundorff, Joshua</creator><creator>Bireley, Stephanie</creator><creator>Coren, Jonathon S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120401</creationdate><title>An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy</title><author>Fuesler, John ; Nagahama, Yasunori ; Szulewski, Joseph ; Mundorff, Joshua ; Bireley, Stephanie ; Coren, Jonathon S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-1ee9475e1ee0d2d5ee15af68a3aa6c44a19bf601540aa0b6dc5c197a1d8fe4533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>animal models</topic><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>chemistry</topic><topic>Cloning, Molecular</topic><topic>Functional genomics</topic><topic>Gene therapy</topic><topic>genes</topic><topic>Genes, p53</topic><topic>Genetic Therapy</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors</topic><topic>genetics</topic><topic>Genome-wide association studies</topic><topic>Genome-Wide Association Study</topic><topic>Genome-Wide Association Study - methods</topic><topic>genomic libraries</topic><topic>Genomic Library</topic><topic>Genomics</topic><topic>Genomics - methods</topic><topic>Green Fluorescent Proteins</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>HapMap Project</topic><topic>human cell lines</topic><topic>Humans</topic><topic>messenger RNA</topic><topic>metabolism</topic><topic>methods</topic><topic>osteosarcoma</topic><topic>Osteosarcoma - metabolism</topic><topic>Protein Biosynthesis</topic><topic>Transcription, Genetic</topic><topic>transfection</topic><topic>Tumor Suppressor Protein p53</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fuesler, John</creatorcontrib><creatorcontrib>Nagahama, Yasunori</creatorcontrib><creatorcontrib>Szulewski, Joseph</creatorcontrib><creatorcontrib>Mundorff, Joshua</creatorcontrib><creatorcontrib>Bireley, Stephanie</creatorcontrib><creatorcontrib>Coren, Jonathon S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fuesler, John</au><au>Nagahama, Yasunori</au><au>Szulewski, Joseph</au><au>Mundorff, Joshua</au><au>Bireley, Stephanie</au><au>Coren, Jonathon S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>496</volume><issue>2</issue><spage>103</spage><epage>109</epage><pages>103-109</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The various iterations of the HapMap Project and many genome-wide association studies (GWAS) have identified hundreds of potential genes involved in monogenic and multifactorial traits. We constructed an arrayed 115,000-member human genomic library in the PAC shuttle vector pJCPAC-Mam2 that can be propagated in both bacterial and human cells. The library appears to represent a two-fold coverage of the human genome. Transient transfection of a p53-containing PAC clone into p53-null Saos-2 human osteosarcoma cells demonstrated that both p53 mRNA and protein were produced. Additionally, expression of the p53 protein triggered apoptosis in a subset of the Saos-2 cells. This library should serve as a valuable resource to validate potential disease genes identified by GWAS in human cell lines and in animal models. Also, individual library members could potentially be used for gene therapy trials for a variety of recessive disorders.
► Human genomic PAC library for functional genomics. ► p53-containing PAC clone is transcribed in p53 null Saos-2 cells. ► p53-containing PAC clone is translated in p53 null Saos-2 cells. ► p53-containing PAC clone causes apoptosis in p53 null Saos-2 cells. ► Human genomic PAC library for gene therapy.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22285925</pmid><doi>10.1016/j.gene.2012.01.011</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 2012-04, Vol.496 (2), p.103-109 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3488463 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | animal models Apoptosis Cell Line, Tumor chemistry Cloning, Molecular Functional genomics Gene therapy genes Genes, p53 Genetic Therapy Genetic Therapy - methods Genetic Vectors genetics Genome-wide association studies Genome-Wide Association Study Genome-Wide Association Study - methods genomic libraries Genomic Library Genomics Genomics - methods Green Fluorescent Proteins Green Fluorescent Proteins - chemistry HapMap Project human cell lines Humans messenger RNA metabolism methods osteosarcoma Osteosarcoma - metabolism Protein Biosynthesis Transcription, Genetic transfection Tumor Suppressor Protein p53 Tumor Suppressor Protein p53 - genetics |
| title | An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T06%3A04%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20arrayed%20human%20genomic%20library%20constructed%20in%20the%20PAC%20shuttle%20vector%20pJCPAC-Mam2%20for%20genome-wide%20association%20studies%20and%20gene%20therapy&rft.jtitle=Gene&rft.au=Fuesler,%20John&rft.date=2012-04-01&rft.volume=496&rft.issue=2&rft.spage=103&rft.epage=109&rft.pages=103-109&rft.issn=0378-1119&rft.eissn=1879-0038&rft_id=info:doi/10.1016/j.gene.2012.01.011&rft_dat=%3Cproquest_pubme%3E922759507%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1672059258&rft_id=info:pmid/22285925&rft_els_id=S0378111912000406&rfr_iscdi=true |