Tamoxifen‐dependent, inducible Hoxb6CreERT recombinase function in lateral plate and limb mesoderm, CNS isthmic organizer, posterior trunk neural crest, hindgut, and tailbud
The ability to generate conditional mutant alleles in mice using Cre‐lox technology has facilitated analysis of genes playing critical roles in multiple developmental processes at different times. We used a transgenic Hoxb6 promoter to drive tamoxifen‐dependent Cre recombinase expression in several...
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| Veröffentlicht in: | Developmental dynamics 2009-02, Vol.238 (2), p.467-474 |
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| creator | Nguyen, Minh‐Thanh Zhu, Jianjian Nakamura, Eiichiro Bao, Xiaozhong Mackem, Susan |
| description | The ability to generate conditional mutant alleles in mice using Cre‐lox technology has facilitated analysis of genes playing critical roles in multiple developmental processes at different times. We used a transgenic Hoxb6 promoter to drive tamoxifen‐dependent Cre recombinase expression in several developing systems that serve as major models for elucidating inductive interactions and mechanisms of morphogenesis, including lateral plate mesoderm and descendant limb buds, neural crest progenitors of the neural tube, tailbud, and CNS isthmic organizer. The Hoxb6CreERT line gives very rapid and complete recombination over a short time window after a single tamoxifen dose, allowing precise time requirements for gene function to be assessed accurately. Embryonic cells cultured from the Hoxb6CreERT line also display rapid recombination ex vivo after tamoxifen exposure. Hence, the Hoxb6CreERT line provides a valuable tool for analyzing gene function, as well as lineage tracing studies using genetic cell marking, in several developing systems. Developmental Dynamics 238:467–474, 2009. Published 2009 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/dvdy.21846 |
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We used a transgenic Hoxb6 promoter to drive tamoxifen‐dependent Cre recombinase expression in several developing systems that serve as major models for elucidating inductive interactions and mechanisms of morphogenesis, including lateral plate mesoderm and descendant limb buds, neural crest progenitors of the neural tube, tailbud, and CNS isthmic organizer. The Hoxb6CreERT line gives very rapid and complete recombination over a short time window after a single tamoxifen dose, allowing precise time requirements for gene function to be assessed accurately. Embryonic cells cultured from the Hoxb6CreERT line also display rapid recombination ex vivo after tamoxifen exposure. Hence, the Hoxb6CreERT line provides a valuable tool for analyzing gene function, as well as lineage tracing studies using genetic cell marking, in several developing systems. Developmental Dynamics 238:467–474, 2009. Published 2009 Wiley‐Liss, Inc.</description><identifier>ISSN: 1058-8388</identifier><identifier>EISSN: 1097-0177</identifier><identifier>DOI: 10.1002/dvdy.21846</identifier><identifier>PMID: 19161221</identifier><language>eng</language><publisher>New York: Wiley‐Liss, Inc</publisher><subject>Animals ; Body Patterning ; Cells, Cultured ; CNS isthmic organizer ; Embryo, Mammalian - metabolism ; Extremities - embryology ; Extremities - physiology ; Gastrointestinal Tract - embryology ; Gastrointestinal Tract - metabolism ; Gene Expression Regulation ; gut endoderm ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Hoxb6 transgenic promoter ; Integrases - genetics ; lateral plate ; limb ; Mesoderm - embryology ; Mesoderm - metabolism ; Mice ; Mice, Transgenic ; neural crest ; Neural Crest - embryology ; Neural Crest - metabolism ; Promoter Regions, Genetic ; Recombination, Genetic ; Tail - embryology ; Tail - metabolism ; tailbud ; Tamoxifen - pharmacology ; tamoxifen‐dependent Cre</subject><ispartof>Developmental dynamics, 2009-02, Vol.238 (2), p.467-474</ispartof><rights>Published 2009 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdvdy.21846$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdvdy.21846$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,1427,27903,27904,45553,45554,46387,46811</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19161221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, Minh‐Thanh</creatorcontrib><creatorcontrib>Zhu, Jianjian</creatorcontrib><creatorcontrib>Nakamura, Eiichiro</creatorcontrib><creatorcontrib>Bao, Xiaozhong</creatorcontrib><creatorcontrib>Mackem, Susan</creatorcontrib><title>Tamoxifen‐dependent, inducible Hoxb6CreERT recombinase function in lateral plate and limb mesoderm, CNS isthmic organizer, posterior trunk neural crest, hindgut, and tailbud</title><title>Developmental dynamics</title><addtitle>Dev Dyn</addtitle><description>The ability to generate conditional mutant alleles in mice using Cre‐lox technology has facilitated analysis of genes playing critical roles in multiple developmental processes at different times. 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Published 2009 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Body Patterning</subject><subject>Cells, Cultured</subject><subject>CNS isthmic organizer</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Extremities - embryology</subject><subject>Extremities - physiology</subject><subject>Gastrointestinal Tract - embryology</subject><subject>Gastrointestinal Tract - metabolism</subject><subject>Gene Expression Regulation</subject><subject>gut endoderm</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Hoxb6 transgenic promoter</subject><subject>Integrases - genetics</subject><subject>lateral plate</subject><subject>limb</subject><subject>Mesoderm - embryology</subject><subject>Mesoderm - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>neural crest</subject><subject>Neural Crest - embryology</subject><subject>Neural Crest - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Recombination, Genetic</subject><subject>Tail - embryology</subject><subject>Tail - metabolism</subject><subject>tailbud</subject><subject>Tamoxifen - pharmacology</subject><subject>tamoxifen‐dependent Cre</subject><issn>1058-8388</issn><issn>1097-0177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAUhSMEoqWw4QGQV6wmxTeZOPEGCU1Li1SBBAMSK8s_NzMuiR3spHS64hF4E96JJ8Fpy9-KlY90j48-X58sewz0ECgtnpkLszssoFmyO9k-UF7nFOr67qyrJm_KptnLHsR4Tilt2BLuZ3vAgUFRwH72fS17f2lbdD--fjM4oDPoxgWxzkzaqg7Jqb9UbBXw-O2aBNS-V9bJiKSdnB6td8lKOjlikB0ZZkGkM6SzvSI9Rm8w9Auyev2O2Dhue6uJDxvp7BWGBRl8TBetD2QMk_tEHE5zjA4YE8M2QWymJObAUdpOTeZhdq-VXcRHt-dB9v7l8Xp1mp-9OXm1enGWn5eMs3xJNVcKoKo1mJZLUMjAFFRhXdWNZIxJ4KWpNIBuObRSMk4ZM5IzXStKy4Ps-U3uMKkejU5LSWRiCLaXYSe8tOLfibNbsfEXolymhddzwNPbgOA_T-k9ordRY9dJh36KgrGmKaua_9dY0IoWvKiS8cnfSL9Zfn1mMsCN4YvtcPdnTsVcEzHXRFzXRBx9OPp4rcqfy9u14A</recordid><startdate>200902</startdate><enddate>200902</enddate><creator>Nguyen, Minh‐Thanh</creator><creator>Zhu, Jianjian</creator><creator>Nakamura, Eiichiro</creator><creator>Bao, Xiaozhong</creator><creator>Mackem, Susan</creator><general>Wiley‐Liss, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200902</creationdate><title>Tamoxifen‐dependent, inducible Hoxb6CreERT recombinase function in lateral plate and limb mesoderm, CNS isthmic organizer, posterior trunk neural crest, hindgut, and tailbud</title><author>Nguyen, Minh‐Thanh ; Zhu, Jianjian ; Nakamura, Eiichiro ; Bao, Xiaozhong ; Mackem, Susan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3696-40c9bb1157c1df9a1be61d20be7578a666a193d5c11cf91faa69066da96c7b003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Body Patterning</topic><topic>Cells, Cultured</topic><topic>CNS isthmic organizer</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Extremities - embryology</topic><topic>Extremities - physiology</topic><topic>Gastrointestinal Tract - embryology</topic><topic>Gastrointestinal Tract - metabolism</topic><topic>Gene Expression Regulation</topic><topic>gut endoderm</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Hoxb6 transgenic promoter</topic><topic>Integrases - genetics</topic><topic>lateral plate</topic><topic>limb</topic><topic>Mesoderm - embryology</topic><topic>Mesoderm - metabolism</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>neural crest</topic><topic>Neural Crest - embryology</topic><topic>Neural Crest - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Recombination, Genetic</topic><topic>Tail - embryology</topic><topic>Tail - metabolism</topic><topic>tailbud</topic><topic>Tamoxifen - pharmacology</topic><topic>tamoxifen‐dependent Cre</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, Minh‐Thanh</creatorcontrib><creatorcontrib>Zhu, Jianjian</creatorcontrib><creatorcontrib>Nakamura, Eiichiro</creatorcontrib><creatorcontrib>Bao, Xiaozhong</creatorcontrib><creatorcontrib>Mackem, Susan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Developmental dynamics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, Minh‐Thanh</au><au>Zhu, Jianjian</au><au>Nakamura, Eiichiro</au><au>Bao, Xiaozhong</au><au>Mackem, Susan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tamoxifen‐dependent, inducible Hoxb6CreERT recombinase function in lateral plate and limb mesoderm, CNS isthmic organizer, posterior trunk neural crest, hindgut, and tailbud</atitle><jtitle>Developmental dynamics</jtitle><addtitle>Dev Dyn</addtitle><date>2009-02</date><risdate>2009</risdate><volume>238</volume><issue>2</issue><spage>467</spage><epage>474</epage><pages>467-474</pages><issn>1058-8388</issn><eissn>1097-0177</eissn><abstract>The ability to generate conditional mutant alleles in mice using Cre‐lox technology has facilitated analysis of genes playing critical roles in multiple developmental processes at different times. We used a transgenic Hoxb6 promoter to drive tamoxifen‐dependent Cre recombinase expression in several developing systems that serve as major models for elucidating inductive interactions and mechanisms of morphogenesis, including lateral plate mesoderm and descendant limb buds, neural crest progenitors of the neural tube, tailbud, and CNS isthmic organizer. The Hoxb6CreERT line gives very rapid and complete recombination over a short time window after a single tamoxifen dose, allowing precise time requirements for gene function to be assessed accurately. Embryonic cells cultured from the Hoxb6CreERT line also display rapid recombination ex vivo after tamoxifen exposure. Hence, the Hoxb6CreERT line provides a valuable tool for analyzing gene function, as well as lineage tracing studies using genetic cell marking, in several developing systems. Developmental Dynamics 238:467–474, 2009. 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| subjects | Animals Body Patterning Cells, Cultured CNS isthmic organizer Embryo, Mammalian - metabolism Extremities - embryology Extremities - physiology Gastrointestinal Tract - embryology Gastrointestinal Tract - metabolism Gene Expression Regulation gut endoderm Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Hoxb6 transgenic promoter Integrases - genetics lateral plate limb Mesoderm - embryology Mesoderm - metabolism Mice Mice, Transgenic neural crest Neural Crest - embryology Neural Crest - metabolism Promoter Regions, Genetic Recombination, Genetic Tail - embryology Tail - metabolism tailbud Tamoxifen - pharmacology tamoxifen‐dependent Cre |
| title | Tamoxifen‐dependent, inducible Hoxb6CreERT recombinase function in lateral plate and limb mesoderm, CNS isthmic organizer, posterior trunk neural crest, hindgut, and tailbud |
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